Viral

origins
Virology I: How viruses work
Week 7, session 4

(antigenic)

Viral Origins

Canyon

Depression
(host protein binding)

T = 1 symmetry
Diameter ~ 25 nm

rep ORF
cap ORF
TR

TR
p5

p19

p40

mRNAs
5' c

An 4.2 kb

Rep 78
5' c

An 3.9 kb

Rep 68

TRL

L
5' c

UL

An 3.6 kb

Rep 52
5' c

OriL

An 3.3 kb

Rep 40

IRL IRS
OriS

S
US

TRS
OriS

An 2.3 kb

5' c

VP2
VP3

AT-rich DNA segments recognized by viral origin

recogniEon proteins, seed assembly of mulE-protein
complexes

Some viral genomes have one ori, others up to three

VP1

2009 ASM Press

Viral origins of DNA replica3on

2009 ASM Press

Origin Recogni3on Proteins

Polyomavirus T binds specically to DNA

Adenovirus pTP binds at terminus and recruits DNA

polymerase

Herpesvirus UL9 protein recruits viral proteins to AT-

rich ori and then unwinds DNA

Papillomavirus E1 binds to ori in presence of E2

AAV Rep68/78 binds at ends and unwinds DNA, also
involved in terminal resoluEon

SV40 large T

T is a species-specic DBP/OBP

-preiniEaEon complexes do not form in the wrong species

-failure to interact with DNA pol-primase

Binds and sequesters cell cycle regulators

-causes cells to enter S phase
2009 ASM Press

Minimal origin

LT binding
A/T
EP
site II

SV40

BPV

5'
3'

ATP

E1
AT rich

LT

E2

3'
5'

Ori
EI

LT

LT

ATP

E2

ADP + Pi
2

Conformational
change of EP sequence

5'
3'

AT rich

3'
5'

EI

ATP
3
ADP + Pi

LT

Topo I

5'
3'

Rp-A
Topo I

LT

3'
5'