MAHARASHTRA AGRICUL rURAL UNIVERSITIES EXAMINATION BOARD, PUNE ¢MFSTER END EXAMINATION B.Tech.(Food Science) Semester : I(New) Academic Year ; 2007-08 Course No. : FIM-111 Title : Fundamentals of Microbiology Credits 2 3(2+1) Day & Date: Saturday, 22.12.2007 ___ Time _: 9.00 to 12.00 Total Marks : 80 Note: 1. Solve ANY FIVE questions from SECTION “A”. 2. All questions from SECTION “B” are compulsory. 3. All questions carry equal marks. 4, Draw neat diagrams wherever necessary. "SECTION “A” 1 What is mutation? Explain the different types of mutation. Q2 Discuss in detail the general characteristics of: a) Actinomycetes and protozoa b) Algae and Rickettsiae 3 Write the contributions of the following scientists to Microbiology. a) Antony van Leeuwenhoek b) Louis Pasteur 4 Describe in brief how control of microorganisms can be achieved by Physical and Chemical methods. Q.5 Write in brief about />) Scope of Microbiology in food industry b) Methods for Microbial culture preservation _G.6 Whats genetic recombination? Deseribe in brief Transduction in bacteria Q7 Write the difference between: a) Capsule and Slime layer. b) Prokaryotic cell and Eukaryotic cell. SECTION “B” Q8 — Define the following terms. 1. Sterilization 2. Lyophilization 3. Symbiosis 4. Incubation period 5. Lichen 6. Chemotherapeutic agents 7. Mesosome 8. D- Value 9. Toxin 10. Active transport (P.T.0.)Q.10 , Fill in the blanks. 1. Cocei are ____ in shape. 2. The teichoic acids are present only in___ bacteria. 3. In_____ flagellar arrangement, flagella are present all over the cell surface. 4, ____ is the movement of an organism in response to chemical stimulus. 5. ____ isthe living matter of cell between the cell membrane and nucleus. 6. ____ isa unit of length used for measurement of size of viruses. 7. ____ are groups of micro-organisms that show both characters of fungi and bacteria. 8. ____ transport involves the pumping of ions or solutes across the cell membrane from lower to higher concentration. 9. ___ yeasts are to grow in high concentration of sugar. 10._____ organisms occur in more than two forms. Match the pairs. “ar «Br 1. Fastidious microbe a) Union of protoplast 2. Gram positive bacteria b) Mass of hyphae 3. Gram negative bacteria c) Acid fast nature 4. Mycobacterium d) Union of haploid nucleus 5. Plasmogamy e) Derive energy from sunlight 6. Oogamy f) Retain crystal violet 7. DNA g) Retain Safaraning 8. Phototrophs h) Elaborate growth requirement 9. Karyogamy i) Carrier of genetic information 10. Mycelium j) Union of egg and sperm SHHHS HSS HS