B.Sc.

Biotechnology
Semester IV
BBT 401: Practicals in Molecular Biology
1
2

4
5
6
7

Total

Requirements of molecular biology laboratory

Criteria for reliability of qualitative experiments, calibration of micropipettes
and handling practice, Importance of clean handling,
sterility and
cleanliness.
Isolation and analysis of bacterial genomic DNA
Isolation and analysis of bacterial genomic DNA by agarose gel
electrophoresis.
Isolation and analysis of eukaryotic genomic DNA
Isolation and analysis of eukaryotic genomic DNA by agarose gel
electrophoresis.
Isolation and analysis of plant genomic DNA
Isolation and analysis of plant genomic DNA by agarose gel electrophoresis.
Isolation and analysis of RNA form celllines
Isolation of RNA and determination of purity.
Analysis of nucleic acid
Determination of purity and tm.
References
1. Principles of Gene Manipulation 6th Edn., Primrose, S. B., Twyman, R.
M. and Old, R. W., Blackwell Publishing, USA (2004).
2. Principles and Techniques of Biochemistry and Molecular Biology
Wilson, K. and Walker, J., Cambridge University Press, New York
(2005).

15 P
1P
1P

2P

4P
3P
3P
1P