B.Sc.

Biotechnology Semester II BBT 202: Practicals in Biochemistry I

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Significance of good laboratory practices (GLP) Safe handling of equipments/instruments. Introduction to volume and weight measurements Personal safety, accuracy and reliability. Preparation of biochemical reagents Safety in the use of acids, alkalies and organic solvents. Distillation of water and ethanol. Preparation of buffers. Isolation of commercially important biomolecules Isolation of starch from corn (separation on the basis of density) Isolation of casein from milk (separation on the basis of isoelectric pH) Extraction of triglycerides from oilseeds (separation on the basis of differential solubility) To determine max and extinction coefficient of biomolecules Tyrosine, purine and pyrimidine using spectrophotometer. Identification of purines from max. Color reactions for identification Amino acids and proteins. Color reactions for identification Sugars and starch. Paper chromatography Separation of amino acids. Thin layer chromatography (TLC) Separation of sugars. Estimation of reducing sugars Determination of glucose by DNSA method. Estimation of proteins Determination of bovine serum albumin by Biuret method. Acid value and saponification value Determination with reference to fatty acids. Adsorption chromatography Separation of leaf pigments. References 1 Introductory Practical Biochemistry, Sawhney, S. K. and Singh, R. Narosa Publishing House, New Delhi (2000). 2 An Introduction to Practical Biochemistry, Plummer, D.T. TataMcGraw Hill Publishing Company, New Delhi (2005). 3 Principles and Techniques of Biochemistry and Molecular Biology, Wilson, K. and Walker, J. Cambridge University Press, New York (2005). 4 Laboratory Manual in Biochemistry, Jayraman, J., New Age International (P) Ltd., New Delhi (2007).

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