Blood Groups

Ex 26

Blood Groups
Antigen agglutinogen
Antibody agglutinin
Reaction: agglutination
ABO
Blood
Type

Ag

Ab

Anti - A

Anti - B

Anti - B

Anti - A

AB

AB

Anti-A, anti-B

Rh
Blood
Type

Anti - D

Rh (+)

Agglutination

Rh (-)

No agglutination

 Material:
Lancet
70% alc
2 slides/member (3members)
Typing sera (anti-A, anti-B, anti-D)
** sharps container
yellow bag

 Procedure:
1. Label slides A, B, D
2. Sterilize finger 70% alc
3. Puncture finger
4. Wipe 1st drop of blood
5. Place 1gtt of blood on
slide A
slide B
slide D
6. Press punctured finger on cotton
7. Add 1gtt of anti A on slide A
add 1gtt of anti B on slide B
add 1gtt anti D on slide D
8. Mix blood and typing sera using applicator stick (1 per slide)
9. Gently tilt slide back and forth for 1min
10.Observe reaction (clumping) under fluorescent microscope
11.Record result