An-Najah National University

Faculty of Science
Microtechnique 25221
Course outline
Instructor: Dr. Majdi Dwikat
Aim of the course
Students, in this course, will learn about the basic principles and
techniques for the preparation of microscopic slides (for light and
electron microscopy) of animals, plants, and bacteria. In addition, they
will also learn about routine and special staining techniques using
normal and abnormal tissue samples. The also learn the cytological
preparation and their importance for the diagnosis of disease
especially cancers. Finally they study and learn several types of
Microscopy focusing on light and electron microscopy.
Reference Books:
Animal tissue technique, by Humason, Gretchen L 4th edi 1979
http://whitewolf.newcastle.edu.au/techinfo/
http://www-medlib.med.utah.edu/WebPath/HISTHTML/HISTO.html

First Hour exam 15 %

Second hour exam 15 %
Final exam 30 %
Practical exam 40 %

1- Introduction
2- Methods of sample preparation.
3- Fixation and fixative
Methods of fixation
Maceration
Postfixation treatment
Decalcification
Washing

4- Tissue processing
Dehydration
Clearing
Infiltration
Embedding
First hour Exam 15 %

5- Microtomy
6- Stains and staining action
Methods of staining
Hematoxylin eosin staining technique
Specific staining methods using special stains

7- Mounting of sections
8- Frozen section and free dryer
Second hour Exam 15 %

9- Cytology
10-Microscopy
Light Microscope
Electron microscope
Scanning Electron Microscope (SEM)
Transmission Electron Microscope (TEM)
Sample preparation for SEM
Sample preparation for TEM
Final Exam 30 %
Practical exam 40% This percent was put depending on the slides the each student prepare