Self test 01

Vr
V0

Hitunglah waktu yang

diperlukan oleh analit untuk
muncul pada kromatogram, jika
diketahui:
Harga K Volume
void

Laju alir
eluen
ml/mnt

10

Self test 02
SOP menyatakan bahwa kolom harus mempunyai
efisiensi > 30.000 lempeng teoritis/m. Manakah kolom
15 cm yang memenuhi spesifikasi di atas?

Waktu tambat (mnt)

W1/2 mnt

6,4

0,2

5,6

0,2

10,5

0,6

Self test 0.4 FI IV stated that betametason 17-valerate must be

resolved from betametason 21-valerate so that Rs value > 1,0.
Which of the following ODS columns meet the spesification?
Column
ODS

Retention time of
betamethasone
21-valerate (min)

Retention time of
betamethasone
17-valerate (min)

Width at base
of bet 21valerate (min)

Width at base
of bet 17valerate (min)

9.5

8.5

0,4

0,5

9.3

8.6

0,4

0,4

TLC plate (labeled)

Aspirinspot
with samples loaded

Ac As C I
Caffeinespot

Aceaminophenspot

Predict the order

of elution for these
compounds.

Ibuprofenspot

pencilmark1cm
frombottom

depthofmobilephase

OH
O

CO2H
O

H3C

CH3
O

Aspirin

HN

O
CH3

Acetaminophen

O
CO2H
Ibuprofen

CH3
N

N
N
CH3
Caffeine