DC LIU CHA

ANTHRANOID

PHAN T
Tn khoa hc:
PTD la hep
Senna angustifolia Mill. = Cassia angustifolia Vahl.,
Phn h Ceasalpinioidae, H Fabaceae
PTD la nhn
Senna acutifolia (Del.) = Cassia acutifolia Del.
Phn h Ceasalpinioidae, H Fabaceae
Nhiu tac gia xem hai loi nay la mt: Senna angustifolia Mill.

C. angustifolia

C. acutifolia
4

PHAN T
- B phn dng: l cht (Folium Sennae).
- Cn dng ht

THNH PHN HA HC
Anthranoid chim 2 - 3 %.
-Dng t do: rhein, aloe emodin, chrysophanol
R2
-Dng glycosid:
O

OH

R1 = R2 = H cc sennidin

- R3 = COOH,

trans

: Sennidin A

- R3 = COOH,

cis

: Sennidin B

- R3 = CH2OH,

trans

: Sennidin C

- R3 = CH2OH,

cis

: Sennidin D

COOH

R3

R1 = R2 = Glu cc Sennosid tng ng

R1

OH

THNH PHN HA HC
-Cc flavonoid: kaempferol, isorhamnetin.
-Cht nha !!!!
Glu OH O
CH3
HO

CH3
Tinnevellin glucosid

Kaempferol

INH TINH (SKLM)

AG: n-propanol EA nc (4:40:30)

AQ: benzen acid acetic (80:20)

NH LNG
- Dch chit (nc) loi tp chuyn sang dng
oxy ha thy phn thu dch
anthraquinon/KOH UV 500 nm
- Cht chun: 1,8-dihydroxyanthraquinon
- Hm lng tnh theo sennosid
1 mg 1,8-dihydroxyanthraquinon = 1.797 mg sennosid

11

TC DNG - CNG DNG

- Ngi Rp dng t TK IX, sau nhp sang
Chu u, hin nay rt ph bin
- Liu dng:
- 2 g: tr tiu ha
- 3 - 4 g: nhun trng
- 5 - 7 g: ty x

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MUNG TRU
Tn khc: Mung lc, cy lc,

Tn khoa hc: Senna alata (L.) Roxb., Fabaceae

= Cassia alata L.,
BPD: L (Folium Cassiae), ht (Semen Cassiae)

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14

THNH PHN HA HC
Trong l, qua, r u c anthranoid:

AQ

chrysophanol, aloe emodin, rhein,

emodin.

AG

Rhein-8-glucosid, physcion-8-glucosid
Aloe-emodin-8-glucosid

Flavo

Kaempferol
Glu

OH

COOH
O

Rhein-8-glucosid
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Qua

AQ

chrysophanol, aloe emodin, rhein,

emodin.

AG

Rhein-8-glucosid, Aloe-emodin-8glucosid

Flavo

Kaempferol

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TC DNG - CNG DNG

- Dng cha lc: gi nt p ln ch nhim
- Cha to bn: dng 4 - 5 g l dng thuc sc
- Cha giun a: phi hp vi ht Trm bu

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I HONG
Dc in Vit Nam IV qui nh:
Rheum palmatum L., Polygonaceae
Rheum officinale Baillon, Polygonaceae
Hoc ging lai ca 2 loi trn.
palmatum: hnh bn tay
officinale: dc dng

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I HONG

M T THC VT

Cy thao ln (2m).
L hnh tim to (30-40cm), c b cha (Polygonaceae)
R. palmatum c thy su hn R. officinale.
Sau 3-4 nm mc 1 ngn thn hnh chy mang nhiu hoa.
Cy a m, mt. Mc cao >1000m. Tt nht 22004000m.
C ngun gc t Trung Quc. Vit Nam cn phai nhp.

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Rheum sp

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Rheum palmatum

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Rheum palmatum

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Rheum palmatum

23

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I HONG

THU HI CH BIN

B phn dng: thn r Rhizoma Rhei

Thn r nhng cy t 3-4 nm tui.
Ma thu, khi cy tn li
Ct b r, thn r gt b v ngoi.
Phi hay sy kh
Ct gi 1 nm mi dng.

Mt s ni cn dng cung l lm bnh.

24

24

I HONG

M T DC LIU

DVN III
Ming hnh da, tr hay ovan
10cm
Mt ngoi mu vng nu, i
khi c nhng am en nht

25

DVN IV
Hnh tr, hnh non, dng cu
hay meo mo khng u hay
nhng phin mng
= 3-10cm
Mt ngoi mu nu vng hay
nu , i khi c nhng m
en nht. Phin c th c nhng
sc en

Vt b c mu cam
Mi c trng
V ng cht

25

Rhizoma Rhei

26

26

2. manh mo mem

1. manh ban

3. si

5. hat tinh bot

4. calci oxalat
hnh cau gai

27

Soi bt

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I HONG

THNH PHN HA HC
Anthranoid 3-5%
Tannin 5-12%, PC (khng thy phn) > PG
V c: Calci oxalat
Tinh bt, pectin, nha

Tannin coi nh tp cht khi nghin cu anthranoid, d tan trong

nc, cn (dung mi thng dng chit xut Anthranoid)

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THNH PHN HA HC

I HONG

AQ t do 1-2 gm: chrysophanol, emodin, physcion, aloe

emodin v rhein.
AG chim 60-70% anthranoid ton phn.
AG ca cc dn cht kh (lu kho 1 nm)

Cc dianthron: cac rheidin, cac palmidin,

OH

OH

OH

COOH

OH

CH3

HO

COOH

OH

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Dirhein

OH

COOH

OH

OH

Rheidin A

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I HONG

NH TNH
Phan ng Borntrager:
0.10g bt + 5ml H2SO4 (BM).
Lc
Lc vi Benzen, Ether (DVN III), cloroform (DVN
IV), dicloromethan (PTN DLiu)
c mu vng.

Ly lp dung mi hu c, lc vi KOH 0.1N, amoniac

10% (DVN). lp kim c mu .
30

30

I HONG

NH TNH

Sc k lp mng:
Pha tnh: silica gel G hot ha 105oC (DVN III), silica
gel F 254 (DVN IV, PTN Dliu).
Pha ng: EP-EA-acid formic (75:25:1) (DVN),
Bz-EA-a. formic (75:24:1) (PTN Dliu).
Dung dch th:
Chit bng nc acid. Lc
Lc vi ether ethylic, c n cn
Ha li trong ether
Chm sc k
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I HONG

NH TNH
Sc k lp mng:
Dung dch i chiu: emodin/ether ethylic nng
1mg/ml hoc dch chit bt H chun.
Chm trn cng ban mng.
Quan st di UV 365nm.
H ban mng trong hi amoniac.

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32

I HONG

TP CHT (DVN IV)

Pht hin bng SKLM.
Pha tnh: Silica gel F254
Pha ng: EA-MeOH-H2O (100:3.5:10)
Dung dch th: chit bng ethnol, BM. Lc, chm
ln ban mng 10L.
Quan st UV 365nm.
Khng c c vt pht hunh quang tm xanh
khoang Rf 0.4-0.6 (vt ca rhaponticosid)

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TP CHT

I HONG

Rhaponticosid xut hin khi co ln tp cac loai: R. rhaponticum

hay R. undulatum.
Rhaponticosid phat hunh quang UV 365.
Co th kim tra bng SKLM vi h
n-butanol-a.acetic-nc (4:1:5).

C
T
hRf = 50 55

70

80

95

mau th T a b lan rhaponticosid

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34

NH LNG
6.1. Phng phap Auterhoff
- thuy phan trc tiep vi AcOH bang, un soi 15 phut
- e nguoi, tach aglycon bang ether soi.
- aglycon cho phan ng vi (NaOH + NH3)
- tach lp kiem (mau o), em o quang (515 nm)
- So sanh vi dung dch CoCl2
- (A cua d.dch CoCl2 1% # A cua d.dch istizin 3,6 ppm)

ai hoang phai cha 2,5% anthranoid, tnh theo Istizin

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35

NH LNG
6.2. Phng phap DVN IV

- chiet m (g) mau bang nc nong cach thuy.

- e nguoi, them Na bicarbonat, lac, loc lay dch
- Oxy hoa bang FeCl3 2% nong, thuy phan (HCl nong)
- chiet aglycon bang ether, can ether + Mg acetat / MeOH

- o o hap thu A 515 nm (khong dung chuan)

- ket qua c tnh trc tiep

X% = 64A / 100m

ai hoang phai cha 2,2% anthranoid (tnh theo rhein)

Thc chat : nh lng cac anthranoid acid

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I HONG

TC DNG
Tng tit dch: tng thai nc, Na v K
Tc dng ln h c trn
Lm tng nhu ng rut nhun, ty.
Tng nhu ng bng quang, t cung PNCT,
b vim bng quang khng nn dng.
Gy sung huyt CC ngi b tr.
Cha nhiu dng kh khng dng DL ti.
Nhiu Calci oxalat thn trng/si thn.
Ung c tc dng chm: 8-12 gi.
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37

I HONG

TC DNG
Tc dng mnh dn theo liu lng:

38

0.05-0.1g

: b, kch thch tiu ha.

0.1-0.15g

: nhun trng.

0.5-2g

: x.

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NHU
Tn khac: nhau nui, cy ngao, cy mt quy, Giu

Tn khoa hoc: Morinda citrifolia L., Rubiaceae

BPD: Toan cy (r, vo thn, la va qua), nhng chu yu la
r.
Qua cng ang c lu y nhiu.

Cng dung:
Cha cao huyt ap, au lng nhc mi.

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NHAU

Ten khac: Cay ngao, Nhau nui, Mat quy

Ten khoa hoc: Morinda citrifolia L.
Ho ca phe (Rubiaceae)
Mo ta thc vat
Cay cao khoang 6-8 met.
La moc oi, phien la to, hnh bau duc, tren bong lang.

Qua phc. Qua co v chua hi khai, an c.

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Nhau nc: Morinda persicaefolia Ham., Rubiaceae

42

Damnacanthal

Nor-damnacanthal

Cac cht co tac dng khang khi u trong r Nhau nui

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H TH
Tn khoa hc: Fallopia multiflora Haraldson
(Polygonum multiforum Thunb.), Polygonaceae
M ta:
Dy leo, la n hinh tim,
R c mau nu o to va nc
B phn dung: r c (Radix Poligoni multiflori)
Thu hai ch bin: ao c vao mua ng, cu chng cu sai
Cng dng:
Lam thuc b mau, cha ru toc bc sm
Cha suy nhc thn kinh, mt ngu hay qun.
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45

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L HI
- Tn khac: Tng am, h thit, Nha am

- Tn khoa hc: Aloe sp., Asphodelaceae (Liliaceae, Aloeaceae)

Hai loai thng dung: Aloe vera L. va Aloe ferox Mill.
- Aloe ferox Mill. cao t 2 - 5m, l mc thnh hoa th dy, di
15 -50cm, rng 10cm gc, c gai mt di l v mp l.
Hoa mu . Ch yu c nam Phi, cho l hi x Cape
- Aloe vera L. (= vulgaris Lam.) thn ngn 30 - 50cm. L ch c
gai 2 mp. Hoa mu vng. Cy ngun gc bc Phi, cho l
hi Barbade.

- BPD: La dung ti hay ly nha th

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Aloe ferox Mill.

Aloe vera L.

47

Aloe ferox Mill.

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50

Cac anthranoid co trong mt s loai Aloe

51

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53

Cac C-Glycosid

54

TC DNG - CNG DNG

- Kich thich tiu hoa ty x tuy liu lng
- Cht nhy dung tr bng, dung trong my phm

55

HT

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