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JABATAN PELAJARAN KELANTAN

JALAN DOKTOR, 15000 KOTA BHARU.


TRIAL EXAMINATION
2012
BIOLOGY (BIOLOGI)
SIJIL TINGGI PERSEKOLAHAN MALAYSIA
(MALAYSIA HIGHER SCHOOL EDUCATION)
PAPER 1 (KERTAS 1)
MULTIPLE-CHOICE (ANEKA PILIHAN)
One hour and forty-five minutes (Satu jam empat puluh lima minit)
Instructions to candidates:
DO NOT OPEN THIS BOOKLET UNTIL YOU TOLD TO DO SO
There are fifty questions in this paper. For each question, four suggested answers are given.
Choose one correct answer and indicate it on the multiple-choice answer sheet provided.
Read the instructions on the multiple-choice answer sheet very carefully.
Answer all questions. Marks will not be deducted for wrong answers.
Arahan kepada calon :
JANGAN BUKA BUKU SOALAN INI SEHINGGA ANDA DIBENARKAN BERBUAT
DEMIKIAN
Ada lima puluh soalan dalam kertas ini. Bagi setiap soalan, empat cadangan jawapan
diberikan. Pilih satu jawapan yang betul dan tandakan jawapan itu pada helaian jawapan
aneka pilihan yang dibekalkan.
Baca arahan pada helaian jawapan aneka pilihan itu dengan teliti.
Jawab semua soalan. Markah tidak akan ditolak bagi jawapan yang salah.
___________________________________________________________________________
This question paper consists of 16 printed pages
(Kertas soalan ini terdiri daripada 16 halaman bercetak)

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Section A
Four suggested answers labeled A, B, C, and D are given for each question. Choose one
correct answer.
1. Which of the following properties of water are important for the temperature regulation in
mammals?
I
High latent heat of vaporisation
II Highest density at 4C
III High heat capacity
IV High surface tension
A I and II
B I and III
C I and IV
D II and IV

2. Proteins can function as a buffer because


A proteins contains peptide bonds
B proteins are non-polar molecules
C proteins are soluble in water
D proteins are amphoteric molecules

3. Saturated fatty acids differ from unsaturated fatty acids in


A the presence of double covalent bond in the fatty acids
B the physical condition of the fatty acid at room temperature
C the number of oxygen atoms in the fatty acids
D the branching or linear shape of the fatty acids

4. Which of the following is correct for the components and their functions?
I
Lecithin acts as a receptor to molecules such as hormone
II Cholesterol maintain the fluid characteristic of the membrane
III Smooth endoplasmic reticulum detoxification in liver cells
IV Phloem translocation of organic products of photosynthesis
A I and II
B II and IV
C I, II and IV
D II, III and IV
5. Which of the following structure is usually found in animal cells?
I
Lysosome
II Plastids
III Microtubules
IV Centrioles
A I and II
B I and III
C I and IV
D I, III and IV
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6. Which of the following statements are true about non-competitive inhibitors?


I
Bind temporarily on the active site of the enzyme
II Reduce the Km value only if the substrate concentration is low
III The Km value of the enzyme remains the same
IV Cyanide is the example of a non-competitive inhibitor
A I and II
B I and IV
C III and IV
D II, III and IV

7. Which of the following is not a stop codon?


A UAA
B UGA
C UAG
D AUG

8. What are the advantages of light independent process in C4 compared to C3 plants?


I
In the bundle sheath cells, the effectiveness of RuBP carboxylase is increased
II PEP carboxylase has higher affinity towards carbon dioxide compared to RuBP
carboxylase
III The first product of the light independent process in C4 is a four carbon molecules
IV Increases photorespiration
A I and II
B I and IV
C II and III
D I, II and IV

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9.

The following diagram shows a scheme for the light reaction of photosynthesis.
Z
primaryacceptor

H+
Y
Pq

2e

ferredoxin

Cytochromecomplex
2e
Pc
PhotosystemI

PhotosystemII
sunlight

X
2e
sunlight
W
Which of the following substances are represented by W, X, Y and Z in the scheme?
W
X
Y
Z
ADP
ATP
O2
A NADP
ADP
ATP
O2
NADPH
B
O2
ADP NADPH
H2 O
C
H2O
O2
ATP NADPH
D

10.

What will become of all carbons from a glucose molecule after it is completely oxidised?
A Pyruvate
B Carbohydrate
C Carbon dioxide
D Acetyl coenzyme A

11. Which of the following statements are true about anaerobic respiration in plants?
I 2 ATP are produced
II 4 ATP are produced
III The end product are ethanol and carbon dioxide
IV NADH is used in the reduction of pyruvate
A I and IV
B II and III
C II, III, and IV
D I, III and IV

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12.

Which of the following is a hydrogen donor in the biosynthesis of purple sulphur bacteria and
green sulphur bacteria?
A Water
B Hydrogen sulphide
C NADH
D Hydrocarbon compound

13.

Changes of hydrogen ion concentration in cerebrospinal fluid in the brain is detected by the
A peripheral chemoreceptor
B central chemoreceptor
C aortic body
D carotid body

14.

In Bohrs effect, the affinity of haemoglobin towards oxygen is reduced because


A haemoglobin acts as pH buffer in erythrocytes
B high concentration of CO2 altered the shape of haemoglobin
C ferum ion in haemoglobin is attracted to chloride ions that diffuses into erythrocytes
D the concentration of carbamino-haemoglobin increases in erythtrocytes

15.

The pressure changes in the aorta, left ventricle and left atrium are shown in the graph below.
In which of the following segments A, B, C or D, is the semilunar valve of aorta open?

16.

Which of the following statements about transpiration pull is true?


I It is an energy dependent process.
II It is caused by evaporation of water molecules through the stoma.
III It is affected by relative density of atmosphere.
IV It involves the movement of water molecules from vessels into the atmosphere.
A II and III
B III and IV
C II, III and IV
D I, II, III and IV

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17. The main function of a stoma to


A respond to the environmental stress
B allow the diffusion of gas into the mesophyll cell
C allow the diffusion of gas while regulating water loss
D regulate the movement of water during transpiration

18. What the is the name given to the route taken by lactate produced in the skeleton muscle
to liver where it is converted into glucose ?
Carried by
bloodstream
to liver
Lactate
pyruvate
A
B
C
D

glucose

glycogen

Calvin Cycle
Cori Cycle
Krebs Cycle
Hatch-Slack Cycle

19.

Which of the following is the effect of ADH on nephrons?


A Increase the permeability of the proximal convoluted tubule to water
B Increase the permeability of the proximal convoluted tubule to Na+ ions
C Increase the permeability of the distal convoluted tubule to water
D Increase the permeability of the distal convoluted tubule to Na+ ions

20.

The following table shows the concentration of the main ions inside and outside of the
nerve cell during resting potential.

Ion
Na+
ClK+

Concentration in the cell mol dm3


15
10
150

Concentration outside cell mol dm3


150
110
5

What would happen when the nerve receives the correct stimulus ?
A
B
C
D

Na+ ions will enter the cell


K+ ion will enter the cell
Na+ ion will leave the cell
Na+ and Cl- ions will enter the cell

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21. Which of the following are the functions of the synapse


I To connect two neurons
II To act as the impulse inhibition site
III To increase the rate of propagation of impulse
IV To prevent excessive stimulation through the fatigue process
I and II
A
I and IV
B
C II and III
D III and IV

22. Cocaine increases the amount of dopamine in synaptic cleft by


A binding to receptor sites on postsynaptic membrane
B binding to transporter protein that returns dopamine into presynaptic neurone
C preventing action of hydrolytic enzymes on dopamine in synaptic cleft
D binding to dopamine to increase its activity

23. Which statement is true about the role of progesterone in the menstrual cycle?
A Stimulates the development of Graafian follicle
B Causes ovulation
C Stimulates the remaining part of the Graafian follicle to develop into the corpus
luteum
D Maintain the thickness of the endometrium

24.
A
C

Green bananas change colour when expose to the hormone


Absisic acid
B Auxin
ethylene
D cytokinine

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25. The table below shows several plant hormones and their roles

Hormone
Gibberellic acid

Role of hormone
(a) To stimulate the transportation of nutrients from the leaves
to other parts of the plant

II Cytokinin

(b) To stimulate the closing of stomata under physiological


stress

III Auxin

(c) To stop the growth of the adventitious root

IV Abscisic acid

(d) As a fruit ripening agent

V Ethene

(e) To stimulate cell division of the lateral cambium during


secondary growth

Which of the following is correct for the hormones and their roles?
I
II
III
IV
V
(b)
(d)
A (c) (a) (e)
(b)
(d)
B (a) (c) (e)
(e)
(d)
C (b) (c) (a)
(e)
(d)
D (b) (a) (c)

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26. The diagram below shows the immune response in human body

Which statement about the diagram is true?


A Cell X excretes lymphokine, then divides to form cells Y and Z in the presence of
Substance T.
B Cell Z responds quickly towards the repeated infection of a particular disease.
C Cell Y prepares other lymphocytes to produce antibodies.
D Substance S regulates lymphocytes involved in the immune response.

27. Which of the following are true of B cell?


I It forms immunity through the humoral response.
II It forms immunity through the cell mediated mechanism.
III It is produced and it achieves maturity in the bone marrow.
IV It is produced in the bone marrow and it achieves maturity in the thymus gland.
Which of the following is correct for the hormones and their roles?
A I and III
B

I and II

II and IV

III and IV

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28 The table below shows three animals and its way of reproduction.

Animal
Rat

Way of reproducing
(a) External fertilisation

II

Frog

(b) Partenogenesis

III Bee

(c) Viviparity

Which of the following is correct match for the ways of reproduction?


I
II
III
A (c) (b) (a)

29

(a) (b)

(c)

(c)

(a)

(b)

(b) (a)

(c)

The table below shows three organs and the germinal layers that formed them.

Organ
Spinal cord

Germinal layer
(a) Endoderm

II Kidney

(b) Mesoderm

III Liver

(c) Ectoderm

Which of the following is correct match for the above three organs and germinal layers that
formed them?
I
II
III
(b)
A (a) (c)
B

(b) (a)

(c)

(b) (c)

(a)

(c) (b)

(a)

30. If a diploid state is represented by D and a haploid state is represented by H,which of the
following is the correct match for the number of chromosome to the parts of plant structures.

A
B
C
D

Polar nucleus
D
D
H
H

Tube nucleus
D
H
D
H

Anther
H
H
H
H

Cell of pericarp
D
D
H
D

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Cell of testa
D
H
H
D
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31

Which class of organism displays an intermittent growth pattern?


A Insecta
B Mammalia
C Reptilia
D Amphibia

32 Which of the following pairs of parents can produce children with O blood group?
I

AxB

II

AxO

III AB x O
A
B
C
D

I and II
I and III
II and III
I,II and III

33 What is allele frequency?


A It is all the alleles at all gene loci in all individuals of the population.
B It is the frequency of each genotype in the population.
C

It is the proportion of the total of a certain allele to the total number of all the
alleles in the gene pool.

It is the proportion of an allele to the total number of phenotypes in the gene


pool.

34 In a population of cows at genetic equilibrium, 750 cows have dominant grey coat,
while the remaining 250 cows have recessive white coat. What is the number of
cows with heterozygous genotype?
A 250
B 500
C 750
D 1000

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35

The cystic fibrosis ailment on the pancreas is a condition of recessive


homozygous. If one in every 2000 individuals in a population suffers from this
ailment, what is the frequency of the gene in that population?
A 0.005
B 0.001
C 0.022
D 0.044

36

The following are the enzymes produced in Escherichia coli


I

transacetylase

II

B-galactosidase

III

lactose permease

Which of the following is the correct match between the structural genes and the
enzymes that they code?
Lac Z

Lac Y

Lac A

II

III

II

III

III

II

III

II

37 What are inducible enzymes?


A Enzymes that are constantly synthesized and are not dependent on the presence
of inducers.
B Enzymes that are regularly produced but can be turned off in the presence of
repressors.
C Enzymes synthesized in the presence of specific molecules called inducers.
D Enzymes that are regularly produced but can be turned off in the presence of
corepressors.

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38 Which of the following sequences can form a palindrome?


A GGCATC
B GGCGCC
C GGCCGG
D GGGCCC

39 The following shows the steps taken during a DNA fingerprinting process.
I DNA treated with endonuclease.
II Fragments separated by gel electrophoresis.
III Radioactive probes with complementary base sequence are added.
IV Exposed to X-ray film.
The correct sequence of the steps above is
A III, I, II, IV
B I, II, III, IV
C II, I, IV, III
D I, II, IV, III

40

Which of the following are the examples of transgenic organism?


I

Pseudomonas bacteria strain that can break down hydrocarbons

II

Sheep that produces milk with human growth hormone

III

Rhizobium bacteria in root nodules that have the ability to fix nitrogen

IV

Plants that are resistant to glyphosate

I, II and III

II, III and IV

I, II and IV

I, II, III and IV

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41

Which of these is the importance of limitation enzyme action in the technology of DNA
recombinant?
A Limiting the cDNA cloning
B Uniting the chromosome and the plasmid
C Ensuring the randomization of the cutting of DNA sequence
D Ensuring the inter complimentaries of the ends of source DNA fragment and
DNA plasmid

42

Taxonomist took out Mucor from the kingdom plantae and put it under the kingdom
Fungi because
A Mucor is mycelial
B Mucor is heterotropic
C Mucor reproduces by means of spores production
D Mucor does not produce flowers, seeds, or fruits

43

The table below shows four organisms and their specific structures
organisms

Specific structures

I Pheretima

(a) Setae

II Euglena

(b) Radula

III Penaeus

(c) Chloroplast

IV Helix

(d) Telson

Which of the following is correct for the organisms and their specific structures?
I
II
III
IV
(c)
(d)
(b)
A (a)
(a)
(c)
(d)
B (b)
(d)
(c)
(a)
C (b)
(a)
(b)
(c)
D (d)
44

Two plants which belong to different families must also belong to different
A Phyla
B Classes
C Order
D Genera

45

Which of the following characteristics is common to both echinoderms and chordates?


A The embryo undergoes radial cleavage
B The gametes are produced by meiosis
C They are triploblastic
D They reproduce sexually only

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46

The diagram below shows the distribution of human blood groups in a population

Human blood group is an example of


A continuous variation
B discontinuous variation
C extrinsic isolation
D intrinsic isolation
47

The diagram represents the gain and loss of energy (arbitral unit) between one leaf and
the air atmosphere. Based on the data given, the Net Primary Production is
A 30 units
B 60 units
C 90 units
D 270 units
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48

The strongest competition between the different species occurs if they reside in the
same
A Biome
B Habitat
C niche
D community

49

The total product or biomass of an organism is determined by the nutrients or resources


that exist in minimum concentration as compared to other resources that needed. Which
of the following correctly explains the above statement?
A Hardy-Weinberg Law
B Gause Law
C Liebigs Law
D Shelfords Law

50

The table below shows the data that is obtained from the capture-recapture technique
for a population of grasshoppers in field
sample

Number

Number of grasshoppers captured,


marked and released in the first capture

120

Number of grasshoppers captured in the


second capture

60

Number of marked grasshoppers


recaptured

40

What is the estimated grasshopper in that field?


A 20
B 80
C 140
D 180

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Identity card number:.

Centre number :

964/2

TRIAL 2012
BIOLOGY
PAPER2
STRUCTURE AND ESSAY
Two and a half hours

JABATAN PELAJARAN NEGERI KELANTAN


SIJIL TINGGI PERSEKOLAHAN MALAYSIA
For examiners use

Instructions to candidates:
DO NOT OPEN THIS QUESTION PAPER UNTIL YOU
ARE TOLD TO DO SO.
Answer all questions in Section A. Write your answers in the
spaces provided.
Answer any four questions in Section B. Write your answers on
the answer sheets provided. Begin each answer on a fresh sheet
of paper and arrange your answers in numerical order. Tie your
answer sheets to this question paper.

Section A
1
2
3
4
Section B

Answers should be illustrated by large and clearly labeled


diagrams wherever suitable.
Answers may be written in either English or Bahasa Malaysia.

Total

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2
Section A [40 marks]
Answer all questions in this section

1. Figure below is a diagrammatic model of a section of cell surface membrane.

(a) (i) State the name usually given to this model.

[1 mark]

(ii) Give two reasons why it is so called.

[2 marks]

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(b) Name the structures labeled A, B and C.

[3 marks]

A :
B :
C :
(c) What is the function of the channel labeled in the diagram?

[1 mark]

(d) Many substances are said to be actively transported across membranes. Explain what
is meant by active transport.

[2 marks]

(e) Suggest one possible function of the carbohydrate chains attached to the surface of
the membrane.

[1 mark]

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2
The diagram below shows a section through a mitochondrion as seen using an electron
microscope.

(a)

Name the parts labelled A, B and C.

[3 marks]

A :..................................................
B :...............
C: ...
(b)

(i)

Use the letters on the diagram to locate the part of the mitochondrion,

where oxidative phosphorylation occur.

[1 mark]

(ii)

Explain briefly what is meant by oxidative phosphorylation.

[3 marks]

....
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(c) Calculate the actual length of the mitochondrion , if the magnification is


x70000. Show your working and give your answer in suitable units.
marks]

[3

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3
The graph below shows the change in membrane potential during the passage of a nerve
impulse.

(a)

(i)

What is meant by the resting membrane potential of an axon?

[1 mark]

(ii)

What is the value of the resting potential?

[1 mark]

(b) Which labelled part of the graph corresponds to:


(i) depolarization of the axon membrane?

[1 mark]

(ii) repolarisation of the axon membrane?

[1 mark]

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(c) Describe briefly what happens to the axon membrane:


(i) At P

[2 marks]

........
(ii) At R

[2 marks]

................
(d) Explain why the membrane is more negative at S.

[2 marks]

..

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4.
The pedigree below shows the inheritance of red-green colour blindness .Red-green
colour blindness is a sex-linked recessive condition. The gene for colour blindness is carried on
the X-chromosome.

(a) Why is X linkage more common than Y linkage?

[2 marks]

(b) Assuming that the alleles C and c control the expression of the trait, state the
genotype of the following individuals labelled A, B, C, D and E.

[5 marks]

A:.
B:.
C:.
D:..
E:..

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(c) By means of a genetic diagram , what is the probability of a red green colur blindness
father and a mother carrying the allele for red green colour blindness having a red
green colour blindness daughter?

[3 marks]

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10

Section B [60 marks]


Answer any four questions in this section
5

(a) Draw and label the general structure of deoxyribonucleic acid (DNA).

[2 marks]

(b) DNA replicates during interphase. Describe this process.

[13 marks]

Describe the process that occurs at the granum of chloroplast.

[15 marks]

7
(a) Describe the process of ultrafiltration and selective reabsorption that occurs in the
kidney.
[11 marks]
(b) Explain how the kidney produces urine that is more concentrated than blood.
[4 marks]

8
Some organisms overcome the harsh conditions of the environment by hibernation,
aestivation or diapauses. Explain the importance of each of the phenomenom for the survival in
animals.
[15 marks]

(a) What is meant by vector in DNA cloning? State the characteristics of this vector.
[5 marks]
(b) Describe how DNA cloning is carried out by bacteria.
[10 marks]

10

(a) Describe the genetic and environmental factors that cause variation in an organism.
[12 marks]
[3 marks]
(b) Explain the importance of variation in a population.

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11

JABATANPELAJARANNEGERIKELANTAN

PEPERIKSAANKENDALIANBERASASKANSEKOLAH(TRIAL)

TINGKATAN6TAHUN2012

MARKINGSCHEME
BIOLOGY(PAPER2)
STRUCTUREANDESSAY

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12

CONFIDENTIAL*

Marking Scheme
1(a)(i)

fluid mosaic model

1
[1 mark]

(ii)

scattered mosaic arrangement of proteins that float in the phospholipid layer

The lipid and protein molecules can move laterally (and rotate on their axis)

1
[2marks]

(b)

A : phopholipid bilayer

B : extrinsic/peripheral protein

C : intrinsic protein

1
[3 marks]

(c)

To allow transport of polar molecules and ions across the membrane by 1


facilitated diffusion
[1 mark]

(d)

Movement of polar molecules/ions across the membrane

Against the concentration gradient

Requires ATP

1
Any 2 pt
[2 marks]

(e)

For cell recognition

To act as receptors/binding sites for chemical signals

For cell adhesion /to stick the correct cells together

1
Any 1 pt
[1 mark]
Total:
[10
marks]

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2
(a)

A : matrix

B : cristae/inner membrane

C : intermembrane space

1
[3 marks]

(b)
(i)

1
[1mark]

(ii) electrons flow through a series of electron carriers/ ETS

electrons are transferred to the final electron acceptor oxygen producing water

ATP is generated from ADP and phosphate inorganic

1
[3 marks]

Length on photograph : 79mm


(c)
Actual length = observed size

Magnification
= 79 x 103 m

70000
= 1.13 m

1
[3 marks]
Total :10

3
(a)(i)

Is the potential difference between the inside and outside of an axon membrane 1
when the axon is in a resting state
[1mark]

(ii) - 70mV

1
[1mark]

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(b)(i)
[1mark]
(ii) R

1
[1mark]

(c) (i)

More Na+ ions channels open //influx/rapid diffusion of Na+ ions into the axon

Membrane is polarized/ potential inside the membrane is positive

1
[2 marks]

(ii) K+ions channels open//K+ ions diffuse out of the axon

Membrane is repolarised/potential inside the membrane becomes negative 1


again
[2 marks]
(d)

Sodium channels are closed and potassium channels remain opened

K+ ions continue to flow out

1
[2 marks]
Total:10

4 (a)

The X chromosome is much larger than Y chromosome

Carries genes for non-sexual characteristics as well as those determining sex

1
[2 marks]

(b)

A : XCY

B : XCXC

C : XCXC

D : XcXc

E : XcY

1
[5 marks]

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(c)

Male

Female
XCXc

(normal but carrier)

Gamet

XC

progeny

XCXc

female carrier

Xc

XcY

red green colour blindness

Xc

XCY
male normal

XcXc

XcY

female RGB

probability of having a red green colour blindness daughter =

maleRGB

1
4

[3 marks]
Total: 10

(a)

Structure of DNA
Label

Diagram

1
[2 marks]

(b)

the method of DNA replication is proposed by Watson and Crick

is known as semi-conservative replication.

the hydrogen bonds between the bases break, the DNA double helix unwind

forming a replication fork

process is catalysed by enzyme helicase

each strand acts as a template for the formation of a new strand

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DNA polymerase catalyses the synthesis of new daughter strand on the template 1
strand continuously

in the 5 to 3 direction

free DNA nucleotides join up to the exposed bases by specific base-pairing

adenine pairs with thymine and cytosine with guanine

the leading strand is synthesized continuously in a direction towards the 1


replication fork

the lagging strand is synthesized as a series of segments called Okazaki fragments

in a direction away from the replication fork

DNA ligase join the sugar-phosphate backbones of Okazaki fragments to form a 1


single strand

specific base pairing has ensured that two identical copies of the original DNA 1
have been formed

each new DNA molecule contains one old and one new strand

16 max 13

process that occurs at the granum of chloroplast is light reaction

reactions requiring light

i.

Light reaction takes place in two pathways i.e cyclic photophosphorylation and

non-cyclic photophosphorylation

Non-cyclic photophosphorylation

PSII/P680 absorbed light energy and the electrons become excited

electrons are accepted by the primary electron acceptors

electrons are then transferred to PS1/P700

through electrons transport system

in chemiosmosis process

PS1/P700 absorbed light energy and the electrons are excited

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electrons are transferred to the primary electrons acceptors

which is then transferred to NADP+ to produce NADPH

water molecules split up/photolysis of water take place

releasing electrons and H+

to replace electrons in PSII/P680

Cyclic photophosphorylation

PSI/P700 absorbed light energy which excite the electrons to a high energy
level

electrons are accepted by the primary electron acceptors

through electrons transport system

electrons are recycled back to PS1/P700

ATP is produced from ADP

1
19 max 15

7 (a)

Ultrafiltration

blood in the glomerulus undergoes ultra-filtration

in the glomerulus hydrostatic pressure is higher compared to the osmotic pressure

caused by the diameter of efferent arteriole which is smaller than the diameter of 1
afferent arteriole

the numerous tiny pores or fenestrations (0.1m in diameter) between the 1


endothelium cells of capillary walls permits many smaller molecules to pass
through

bigger molecules such as plasma protein and red blood cells are retained

in the blood

glomerular filtrate has a chemical composition similar to that of the blood plasma /
contains glucose, amino acids, vitamins, some hormones, urea, uric acid
,creatinine, ions and water but without white and red blood cells, platelets and

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18

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plasma protein molecules such as albumins and globulins


6 Max 4

Reabsorption

all glucose and amino acids are actively reabsorbed from the filtrate into the

proximal convoluted tubule cells

some sodium ions are actively reabsorbed here

a large volume of water are reabsorbed by osmosis from the tubule to the blood

(the descending limb is permeable to water)

water is drawn out by osmosis to the interstitial fluid in the medulla

the loss of water concentrates Na+ and Cl- in the descending limb

(the ascending limb is impermeable to water)

sodium and chloride ions diffuse out from the thin segment

the thick ascending limb actively transport sodium and chloride ions from the 1
filtrate into the medulla

this raises the osmotic concentration of the tissue fluid in the medulla

as a result water is reabsorbed from the collecting duct and descending limb by 1
osmosis

the loop of Henle functions as a countercurrent multiplier.

active reabsorption of sodium ,chloride and calcium ions ions occur in the distal 1
convoluted tubule

11 max 7

(b)

Production of urine which is more concentrated than blood is

(b)

due to uptake of water by osmosis from the glomerular filtrate into the blood 1
cappilaries

occurs as a result of increased permeabilities of the walls of convoluted distal 1


tubule and collecting ducts to water

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19

caused by the presence of ADH/anti-diuretic hormone

diffusion of urea out of the collecting ducts into the interstitial region of the 1
medulla
1
increases the osmotic concentration resulting in the removal of water.

5 max 4
Total 15

Hibernation

isaperiodofrelativelylowmetabolicactivity

associatedwithperiodsoflowtemperature/inwinter

enableshibernatingspeciesofamphibian,reptiles,birdsandmammals

tosurviveattimeswhenfoodisinshortsupply/tosaveitsstoredfood

byreducingtheirenergydemandstoalowlevel

involvesafallinbodytemperaturetothatofenvironment

and a reduction in heart rate, ventilation rate, metabolic rate, growth and 1
development
1
Storedfatinthemammalstissuesareusedtoprovideenergy

for endotherm, hibernation is a way to maintain body temperature during cold 1


season
9 max 5

Aestivation

as response to drought season/hot/dry


eg: lungfish/amphibian/snail/worm/insect

can live in dry swamp/drought/summer by digging/move in mud

undergoaperiodofmetabolicinactivity

secrete a layer of slime which open on its lips to breathe atmosphericair

whichenablesthemtosurviveconditionsofextremedehydration

during aestivation period, the stored fat and part of muscle tissue/protein is used

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20

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7 max5

Diapause

Isaformofarresteddevelopmentshownbyinsects

Canoccuratanystageinthelifecycle:egg,larva,pupaoradult.

Main factors inducing diapause: daylength(photoperiod), food


availability,temperatureandmoisture.

Example:theSilkwormBombyxmorihasanembryonicdiapause(eggstage),
enterdiapausewhenexposetoperiodsoflongdaylength.

diapause occur to overcome unfavourable condition

organised its life cycle with environmental condition/food/light period/ organised


season/chances/mating period

1
1

6 max 5
Total :15

9 (a)

Vectors are:
- DNA molecules that is used to carry foreign DNA into a host cell.

- Examples : plasmids and the bacteriophage .

[2 marks]

Characteristics

- must be able to replicate its own DNA.

- must at least has one specific restriction sites that can be recognized by

restriction enzymes

(b)

- must carry at least a selectable marker gene; eg ampR (antibiotic resistance)

- can be easily extracted from its host/easier to insert into bacteria

- very small in size

5 max 3

Total: 5

- target DNA from the donor cells are isolated /extracted

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- target DNA cleaved/cut into fragments

- by specific restriction / endonuclease enzymes

- DNA fragments require vectors/plasmids

- plasmids are cut/cleaved by the same restriction enzyme

- DNA fragments are inserted into the plasmid

- forming recombinant DNA

- using DNA ligase

- recombinant DNA are inserted into bacterial host cells

- by transformation /transduction

- amplification of recombinant plasmids/ copies of recombinant plasmids are

produced by replication
- screening of recombinant DNA

- the recombinant DNA /required plasmids are extracted from the bacterial

cell

13 max 10

Total :15

10 (a)

Geneticfactors
1

Sexualreproduction

sexualreproductioninvolvestheprocessesofmeiosisduringgameteformation

meiosisallowsgenestobereshuffled

occursduringprophaseIandmetaphaseI

during prophase I, crossing over occurs resulting in the exchange of genetic


materialsbetweentwohomologouschromosomes
duringmetaphaseI,segregationofchromosomesoccurs

combination of these two processes produces a wide variety of different


gametes.
fertilizationbetweenthevariedmaleandfemalegameteisrandom

1
1
1
1
1

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22

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zygoteproducedcontainsnumerouscombinationofgenotypes

Mutation

Geneandchromosomalmutation

Producesachangeinthegenotypes

whichcausestheappearanceofanewcharacteristics

1
1

Hybridisation

Acrossbetweenindividualbelongingtotwodifferentvarieties

producesindividualswithdifferenttraitsfromtheparents

generatingmorevariation

17 max 9

Environmentalfactors

Contributestovariablegeneexpressionamongindividuals

canonlycausechangesinthephenotype

Thecharacteristicsarenotinherited

Variationduetoenvironmentalsourcesisreversible

4 max3

Toatal: 12

(b)

Importanceofvariation:
Causessome individualsin a population to be better adapted for survival than
others

Enablesapopulationtoinhabitalargerrangeofhabitatandniche

A population with little variability has reduced capacity to adapt to


environmentalchanges
whichmayleadtotheextinctionofthepopulationorthewholespecies

1
1
1

isconsideredastherawmaterialfortheprocessofnaturalselection.

5 max 3

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CONFIDENTIAL*

23
Total:15

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Scheme : Biology Paper 1


1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25

B
D
A
B
C
D
D
A
D
C
D
B
B
A
B
C
D
B
C
B
A
B
D
C
A

26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50

B
A
C
D
D
A
A
C
B
C
A
C
D
B
B
D
B
A
D
A
B
B
C
D
D

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