S thnh lp Hemoglobin

Kreb
A.acetic
-Keto
s

Glycin

glutaric

Protoporphyrin III + Fe2+

4.66%
4 Heme
5%

Pyrole

Protoporphyrin

Heme

0.34%
Globin
94%

Hemoglobin

Globin chain synthesis

Week 7 Month 3 : Hb Gower
( 2 2);
Hb Pozland ( 2 2)
Month 3 Month 6 : HbF ( 2 2)
Month 6 Infant : HbF ; HbA
Adult : HbA ( 2 2) >95%
( 2 2) <3%

HbA2

 ngha xt nghim
Mt trong 3 xt nghim cn bn nhm nh gi
tnh trng thiu mu ( Hct, RBC, Hb )
Hct
MCV =
RBC

(fl)

Hb
MCH =
(pg)
RBC
Hb
MCHC =
(g/L)
Hct

PHNG PHP O HEMOGLOBIN

Sahli-Hellige

PP So mu

Cyanmethemoglobin

PP o quang

nh lng hemoglobin
bng phng php so mu
(Sahli-Hellige)

1- Nguyn tc
Hb (mu ton phn)
HCl 0.1N 10
Acid Hematine
ED Pha long
So mu a.Hematine / T sc k
c kt qu trc tip trn ng nghim (g/dl)

2- Dng c
ng nghim Sahli (g%)

B
so
mu
Sahli
Hellige

a thy tinh

T sc k

Pipette Sahli

3- Ho cht

Chlohydric
acid 0.1N

Nc
Ct

4- Bnh phm

Ma mao qun

hoc mu tnh mch

Nh HCl 0.1N vo ng
nghim Sahli n vch
2g%

Bcc 1: Chun b

Kim tra tn BN
& lc u ng mu

Bc 2: Tin hnh phn ng

Dng pipette Sahli ht
20L mu
Lau sch mu dnh
ngoi pipette.

Phn phi mu vo
ng nghim c sn
HCl 0.1N

Dng a thy tinh

trn u mu & HCl
0.1N

Lu :
Ht dung dch trong ng nghim trng
sch mu trong pipette 2-3 ln
Trong qu trnh phn phi mu & trn
u, trnh khng cho ni bt trong ng
nghim.

 yn trong 10pht

Sau 10, 95% Hb bin

thnh a.Hematine mu
nu sm

Tip tc pha

1.

long nu
mu phn
ng cha

2.

trng vi mu
thy tinh mu

1.Pha long bng

nc ct
2.Trn u
3.So mu vi thy
tinh mu

3.

Bc 3: c kt qu
c kt qu trc tip
trn thn ng nghim
(con s nm ngang vi
mt y cong ca dung
dch trong ng nghim)
n v tnh: g% g/100ml)

6- Bin lun
Tr s bnh thung:
Tr em:
+ S sinh thng: 13.5 2 g/dl
+ Tr 3 thng tui : 9.5 2 g/dl
+ Tr em 1 tui

: 12.0 2 g/dl

Ngi ln:
+ Nam : 14 - 18 g/dl (16 2)
+ N : 12 - 16 g/dl (14 2)

6- Bien luan
Hemoglobin gim: thiu mu
Hemoglonin tng:
+ a hng cu.
+ Hb tng gi: khi thc hin bng pp Sahli vi mu
mu c WBC >100.000/mm3 KQ Hb tng 5-10% so
vi gi tr thc.
Lu : HbF cu bo thai, Met-Hb v mt s dn xut
ca Hb khng bin thnh acid Hematine.

7- Nguyn nhn sai lm

Do dng c: thy tinh mu khng chun.
Do ha cht: HCl qu thi hn, khng ng nng .
Do k thut:
ng nghim c bt.
c kt qu vi v.
So mu khng iu kin nh sng.