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MolecularInfo
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Molecular Techniques and Methods

1 M Sodium Phosphate Buffer (pH 5.8-8.0)

Copy Right 2001/ Institute of Molecular Development LLC

1 M NaH2PO4

8.0

1 M Na2HPO4
93.2 ml

7.8
7.6

89.6 ml
84.5 ml

10.4 ml
15.5 ml

7.4

77.4 ml

22.6 ml

7.2
7.0

68.4 ml
57.7 ml

31.6 ml
42.3 ml

6.8
6.6

46.3 ml
35.2 ml

53.7 ml
64.8 ml

6.4

25.5 ml

74.5 ml

6.2
6.0

17.8 ml
12.0 ml

82.2 ml
88.0 ml

5.8

7.9nml

92.1 ml

pH

6.8 ml

Adjust pH with NaH2PO4 and Na2HPO4.

Autoclave and store at room temperature.

1 M Na2HPO4 (100 ml)

Na2HPO4

14.2 g

Add distilled H2O to make a final volume of

100 ml

1 M NaH2PO4 (100 ml)

NaH2PO4

12 g

Add distilled H2O to make a final volume of

100 ml

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