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1. Principle
The method is based on lactose reducing a cupric salt complex to Cu2O under specified conditions. The
amount of Cu2O precipitated is proportional to the lactose content.
2. Scope
The method is used on all milk and whey powders.
3. Apparatus
4. Chemicals
1. Fehling I.
Copper(II)sulphate solution.
Dissolve 34.639 g CuSO4, 5H2O in deionized water.
Dilute to 500.0 ml in a volumetric flask, and filter through a paper filter.
Store in a 500 ml brown glass bottle.
2. Fehling II.
Alkaline tartrate solution.
Dissolve 173.0 ± 0.1 g C4H4Na2O6, H2O and 50.0 ± 0.1 g NaOH pellets in deionized water and
dilute to 500 ml in a volumetric flask.
Allow to stand for 2 days, before filtering through a paper filter.
Store in a 500 ml brown glass bottle.
3. Potassium hydroxide solution.
Dissolve 15.567 g KOH in deionized water. Dilute to 500.0 ml in a volumetric flask.
6. Procedure
7. Result
Use the Hammond Table to express the weight of lactose equivalent to the weight of Cu2O.
20 13,6 76 51,7
30 20,2 77 52,4
40 27,2 78 53,0
50 34,0 79 53,7
51 34,7 80 54,4
52 35,4 81 55,1
53 36,0 82 55,8
54 36,7 83 56,4
55 37,4 84 57,1
56 38,1 85 57,8
57 38,8 86 58,5
58 39,4 87 59,2
59 40,1 88 59,8
60 40,8 89 60,5
61 41,5 90 61,2
62 42,2 91 61,9
63 42,9 92 62,6
64 43,5 93 63,2
65 44,2 94 63,9
66 44,9 95 64,6
67 45,6 96 65,3
68 46,2 97 66,0
69 46,9 98 66,6
70 47,6 99 67,3