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08.13 - PH 121 - Trans #1 - The Cell
08.13 - PH 121 - Trans #1 - The Cell
13 August 2014
The Cell
LECTURE
I. How are specimens prepared for light
microscopy?
II. The Cell
a. Definition
b. Two Basic Compartments
c. Components of the Cell
d. Structure-Function Correlations of the Cell
LABORATORY
I. Staining of Histologic Structures
II. Slides
LECTURELECTURE
I. SPECIMEN PREPARATION
1) Fixation
To preserve tissue morphology and
prevent cell digestion
Use of fixative
A snapshot of what it is
2) Dehydration
To remove cell water (to prevent decay)
Use of ethyl alcohol
3) Clearing
Use of lipid solvent like benzene or
xylene
Tissue becomes transparent
White spots = where fats were once
located
4) Embedding
Baked in paraffin (a white colorless soft
solid) at 58-60 C
Makes tissue more firm for sectioning
5) Sectioning
Use of microtome (cutting tool used to
cut extremely thin slices of material)
Tissue is cut for Light Microscope (LM) :
3-8m & Electron Microscope (EM):
0.02-1 m; section = resolution
6) Staining
Hematoxylin: Basic dye (basophilic)
Purplish Blue
Eosin: Acidic dye (acidophilic) Bright
Pink
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The Cell
II. The Cell
A. DEFINITION
Cell - the smallest unit of protoplasm capable of an
independent existence
Cell Tissue Organ Organ System
B. TWO BASIC COMPONENTS
1. Nucleus
Repository of the cells genetic material
(DNA)
Tells the cell what to do; How long its
lifespan will be
a. Chromatin
Nucleic acids (Deoxyribonucleic acid, DNA):
genetic material of the nucleus, resides in the
chromosomes
c. Nucleoli
Deeply stained by basic dyes due to
ribonucleoprotein
Often surrounded by an intensely
stained rim of nucleololus-associated
chromatin
Usually large in growing cells that are
making large amounts of tissue proteins
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The Cell
b. Ribosomes
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The Cell
- Similarities with bacteria
1) Own protein synthesis machinery
(ribosomes) more like bacteria than
eukaryotes (sensitivity to
Streptomycin)
2) Inner membrane of mitochondria
contains unusual phospholipid
characteristic of bacterial
membranes.
Parts of the Mitochondria:
2) Spaces
a. Intercristal Space: occupied by the
mitochondrial matrix
b. Intracristal Space/Membrane Space
e. Lysosomes
Types:
1) Primary: has not yet digested
2) Secondary: has digested or is
digesting
3. Cytoskeleton
Structural support for the plasma membrane
and cellular organelles
Means for movement of cellular organelles
Movement of specialized structures such as
cilia and flagella
Muscle contractility
Three types of rods in cytoskeleton:
microfilaments, intermediate filaments and
microtubules (all are non-membranous)
4. Cell Membrane
f. Peroxisomes
2. Cytoplasmic Inclusions
1) Membrane
a. Inner Membrane: cristae mitochondriales;
impermeable
b. Outer Membrane: contains
transmembrane channels
AKA plasmalemma
A 2D viscous solutionallows for lateral
movement of unbound proteins
Tight junctions: prevent apicobasal movement of cells (lock cells
in place/proper orientation)
Consists of bimolecular layer of mixed
phospholipids with the hydrophilic ends
oriented outward, and various embedded
integral proteins; Fluid mosaic model
Regulates traffic in and out of cell
Ions, gases, small uncharged
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The Cell
D. STRUCTURE-FUNCTION CORRELATIONS
OF THE CELL
1. Cell Motility (Mobile vs. Sessile)
Mobile: free cells; migrate as needed
Sessile: in close contact (ex.
Epithelia). Movement limited to
changes in surface configuration
via microvilli or cytoplasmic
streaming
2. Energy Production vs. Consumption
3. Electrical Conduction
4. Exocytosis
Release of secretory products (ex.
Glandular cells)
Secretory products may be
enclosed in granules,
released upon cells
reception of proper stimulus.
5. Digestion - through lysosomes
6. Transport through the cytoskeleton
7. Constantly Dividing vs. Permanent
Cells
Constant division: growth, renewal,
repair
Proliferation rates vary
o Rapid proliferation: cell
cycle
Two types of division: mitosis
(somatic cells), meiosis
(germ cells)
No cell division in postnatal life
(permanent)
Cardiac muscle, nervous
tissue
LABORATORY
I. STAINING HISTOLOGIC STRUCTURES
TYPES OF STAIN
1. H&E go together in most staining procedures.
a. Hematoxylin = Purplish blue
b. Eosin = Bright pink
2. Silver stain = Black stain; Silver staining aids
visualization of intracellular and extracellular
cellular components (DNA and proteins) by
deposition of metallic silver particles on the
targets (usually Reticular fibers, nerve fibers,
fungi); argentaffin/arygrophilic substances
stain black with silver
3. Orcein stain = commonly used for staining
elastic fibers that color dark brown
4. Giemsa stain = used to differentiate nuclear
and/or cytoplasmic morphology of platelets
(blue), RBCs (pink/red), WBCs (blue), and
parasites
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The Cell
II. SLIDES
Ciliated:
A. Plasmalemma
Smooth & sharply demarcated:
Flagellated:
Fuzzy:
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The Cell
Brush or striated:
B. Cytoplasm
Basophilic:
Sterocillia:
Acidophilic:
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The Cell
C. Organelles
Fibrils:
RER, Ribosomes:
D. Inclusions
Secretory granules/droplets:
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The Cell
Pigment granules
Fat vacuole
Phagocytosed matter
Figure 2: Slide 1
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The Cell
H. Nucleoplasm can be found in all cells
I.
Nuclear Number
K. Position of Nucleus
J. Nuclear Size
L. Nuclear Shape
Reference
Marieb, E. & Hoehn, K. (2011). Essentials of Human
Anatomy and Physiology (9th ed.). Pearson Education
Inc.
Pacual, J.L. The Cell. Power point
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