ABSTRACT

Prolonged consumption of NSAIDs like Aspirin brings about acid peptic disease in the
long haul. Backbone therapy of NSAIDs prompted gastropathy is proton pumps inhibitors like
Omeprazole, but it comes with a multi-dimensioned untoward reaction package. In the inquest of
safer and equally effective regimen, this study was designed in animal model of rabbitsin which
antiulcer role of Verapamil was assessed alone and in combination of Ranitidine and was
compared with Omeprazole. Thirty six rabbits were split into six groups, each comprise of six
rabbits. Weight of rabbits was measured and dose was calculated accordingly. A single ulcer
producing dose of aspirin (150mg/ kg) was given orally. The first protective group was
administered 50mg/kg ranitidine orally, second was given Verapamil 08mg/kg orally, third
received I/V Omeprazole at the rate of 08mg/kg and fourth was co-administered verapamil and
ranitidine orally with similar doses described above. Rabbits were sacrificed 01 hour after
ulcerogenic dose of aspirin and stomachs were recovered for ulcer index, pH, and gastric acid
contents evaluation and were referred for histopathology. Statistical analysis was carried out by
using Microsoft Office Excel 2013 and SPSS version 21. Students t test and one way
ANOVA, followed by post Hoc Tukey test was applied for ulcer indices and biochemical
parameters, while Chi Square test was used for histopathological comparison. Significant
gastropathy was seen in toxic groups, with substantial elevation in ulcer index, gastric acid
contents and distinctly low pH. Ulcer protection was thoroughgoing with omeprazole. With
ranitidine and verapamil alone groups it was moderate and slight respectively. Insignificant
difference of ulceroprotective effect was pragmatic when omeprazole and combined effects of
verapamil and ranitidine were compared. Hence, it was concluded that ranitidine and verapamil

combination was fairly effective and rationally safer regimen than omeprazole in ulcer protection
of NSAIDs induced gastric injury.
KEY WORDS: Aspirin, Ranitidine, Verapamil, Omeprazole, Gastropathy.