EFFICACY OF

PEROXYACETIC ACID
TIME VS. CONCENTRATION
VS. TEMPERATURE AGAINST
BACTERIA, YEASTS, MOLDS, AND SPORES

Intro
These charts include 6 bacteria species, 5 yeasts, 4 molds, and 6 types of
spores. The 100% Kill Times represent the time to completely eradicate
(100%) of each indicated species. This chart is unusual in that it does not
report the time needed to reduce the given organism to particular log
reductions, which typically would be 5-6 logs.
All challenge organisms started with levels of the challenge from 107 to
108 populations.
These challenge tests would be appropriate for peroxyacetic acid
formulas containing approx. 5.5% PAA and 26-27% hydrogen peroxide.
Other formulas may not perform to the standards and results reported
herein.

Staphylococcus aureus-GP bacteria

100% Kill Time of Staphylococcus aureus

(gram-positive bacteria)
5.5

Elapsed Time (mins.)

5.0
4.5

41 F

4.0

50 F
68 F

3.5

104 F

3.0
2.5
2.0
1.5
1.0
0.5
0.0

110

275

Active PAA (ppm)

Streptococcus faecalis-GP bacteria

100% Kill Time of Streptococcus faecalis

(gram-positive bacteria)

Elapsed Time (mins.)

3.0
41 F

2.5

50 F
68 F

2.0

104 F

1.5
1.0
0.5
0.0

110

275

Active PAA (ppm)

Aerobacter aerogenes-GN bacteria

100% Kill Time of Aerobacter aerogenes

(gram-negative bacteria)
1.5

Elapsed Time (mins.)

41 F
50 F
68 F
104 F

1.0

0.5

0.0

110

275

Active PAA (ppm)

Pseudomonas aeruginosa-GN bacteria

100% Kill Time of Pseudomonas aeruginosa

(gram-negative bacteria)

Elapsed Time (mins.)

3.0

41 F
50 F

2.5

68 F

2.0

104 F

1.5
1.0
0.5
0.0

110

275

Active PAA (ppm)

Salmonella typhimurium-GN bacteria

100% Kill Time of Salmonella typhimurium

(gram-negative bacteria)
3.0

41 F

Elapsed Time (mins.)

50 F

2.5

68 F
104 F

2.0
1.5
1.0
0.5
0.0

110

275

Active PAA (ppm)

Salmonella dublin-GN bacteria

100% Kill Time of Salmonella dublin

(gram-negative bacteria)

Elapsed Time (mins.)

3.0
41 F

2.5

50 F
68 F

2.0

104 F

1.5
1.0
0.5
0.0

110

275

Active PAA (ppm)

Sacchromyces cerevisiae-yeast

100% Kill Time of Sacchromyces cerevisiae

(yeast)

Elapsed Time (mins.)

25.0
41 F

20.0

50 F
68 F

15.0

104 F

10.0
5.0
0.0

110

275

Active PAA (ppm)

Sacchromyces diastaticus-yeast

100% Kill Time of Sacchromyces diastaticus

(yeast)
6

Elapsed Time (mins.)

41 F

50 F
68 F

104 F

3
2
1
0

110

275

Active PAA (ppm)

Candida mycoderma-yeast

100% Kill Time of Candida mycoderma

(yeast)

Elapsed Time (mins.)

140
41 F

120

50 F

100

68 F
104 F

80
60
40
20
0

110

275

Active PAA (ppm)

Hansenula anomala-yeast

100% Kill Time of Hansenula anomala

(yeast)

Elapsed Time (mins.)

140
120

41 F

100

68 F

50 F

104 F

80
60
40
20
0
110

275

Active PAA (ppm)

Pichia membranaefaciens-yeast

100% Kill Time of Pichia membranaefaciens

(yeast)

Elapsed Time (mins.)

140
41 F

120

50 F
68 F

100

104 F

80
60
40
20
0

110

275

Active PAA (ppm)

Penicillium camerunense-mold

100% Kill Time of Penicillium camerunense

(mold)
140

Elapsed Time (mins.)

41 F

120

50 F
68 F

100

104 F

80
60
40
20
0

110

275

Active PAA (ppm)

Aspergillus niger-mold

100% Kill Time of Aspergillus niger

(mold)
300

Elapsed Time (mins.)

41 F
50 F

250

68 F
104 F

200
150
100
50
0

110

275

Active PAA (ppm)

Mucor spececium-mold

100% Kill Time of Mucor spececium

(mold)

Elapsed Time (mins.)

300

41 F
50 F

250

68 F
104 F

200
150
100
50
0

110

275

Active PAA (ppm)

Geotrichum candidum-mold

100% Kill Time of Geotrichum candidum

(mold)
70

Elapsed Time (mins.)

41 F

60

50 F
68 F

50

104 F

40
30
20
10
0

110

275

Active PAA (ppm)

Bacillus cereus-spores

100% Kill Time of Bacillus cereus

(spores)

Elapsed Time (mins.)

70
60

41 F

50

68 F

50 F
104 F

40
30
20
10
0

275

412.5

Active PAA (ppm)

550

Bacillus subtilis-spores

100% Kill Time of Bacillus subtilis

(spores)

Elapsed Time (mins.)

70

41 F

60

50 F
68 F

50

104 F

40
30
20
10
0

275

412.5

Active PAA (ppm)

550

Bacillus mesentericus-spores

100% Kill Time of Bacillus mesentericus

(spores)

Elapsed Time (mins.)

70
60

41 F
50 F

50

68 F
104 F

40
30
20
10
0

275

412.5

Active PAA (ppm)

550

Thermophilic species-spores

100% Kill Time of Thermophilic spore-formers

70

Elapsed Time (mins.)

41 F

60

50 F
68 F

50

104 F

40
30
20
10
0

275

412.5

Active PAA (ppm)

550

Clostridium perfringens-spores

100% Kill Time of Clostridium perfringens

(spores)

Elapsed Time (mins.)

70
60

41 F

50

68 F

50 F

104 F

40
30
20
10
0

275

412.5

Active PAA (ppm)

550

Clostridium specialis-spores

100% Kill Time of Clostridium spec.

(spores)

Elapsed Time (mins.)

45
41 F

40

50 F

35

68 F

30

104 F

25
20
15
10
5
0

275

412.5

Active PAA (ppm)

550