Blood

count
Lab 4
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:Blood count
The complete blood count
or CBC test is used as a
broad screening test to
check for such diseases
as anemia, infection,
and other . which
examines different parts
of the blood.we can use
hemacytometer and
microscope at counting
.blood
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Hemocytometer
The hemocytometer is a device
designed for the counting of blood
cells. It is now also used to count
other types of cells

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hemocytometer consisting of 2 pipettes

one for the red cells and other one for
the white cells and A thick glass slide is
on average 25 theater box. Each square
is divided, including 16 square smaller ,It
was invented by Louis-Charles Malassez

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Red Cell Count

This measurement is made with a
microscope and hemacytometer. The
RBC is recorded as millions of cells
.per cubic millimeter

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The objective of the

:experiment
Set the number of red blood cells in a blood
sample taken from a human using
.Haemocytometer

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The principle of the experiment

Rely on the experience of bloodthinning solution reduce red blood
cells (a solution of isotonic sodium
chloride, 0.9)

Nacl

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:Tools used in the experiment

Haemocytometer - 1
2 - solution isotonic to reduce red
blood cells
3 - covers slides
Nacl
4 - microscope

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:Experiment steps
attending the solution isotonic to - 1
the blood-thinning, which is a sodium
.chloride dissolved in distilled water

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Nacl

drag by sucking the blood is 0.5 Mottaglt sign - 2

up then complete the solution of equal tension
in order to mark 101. Shake and then pipette
.to mix the blood with solution of equal tension

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get rid of a few drops of diluted blood - 3

then put a drop of blood at the edge of
the glass cover subject on the slide.
Leave the slide for two minutes to
precipitate the red blood cells and then
.placed under a microscope to examine

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make small screening force to determine - 4

the distribution of red blood cells
5 - The count of red blood cells use a big
lens. Is the number in five large boxes.
Note that all the big box contains 16 small
.square, which is the count in 80 small box

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:Method of calculation
The total number of red blood cells in
80 small square = Q
The size of the small box = 1 / 5 X 1 /
5 X 1 / 10 = 1 / 250 mm3
Mitigation = 1: 200
The number of red blood cells in 1
mm 3 of blood = Q / 5 X 250 X 200
red blood cell / mm 3 of blood
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White Blood Cell Count

The white blood cell count (WBC) is the
total number of leukocytes in a
volume of blood, expressed as
thousands/l. As with the RBC, the
WBC can be done by manual
methods or by automated cell
counters

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:The objective of the experiment

Set the number of white blood cells in a
sample of blood taken from human use
Hemocytometer

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Tools used in the

:experiment
Hemocytometer - 1
2 - solution relieve the white blood cells
and consists of:
A - 3 ml acetic acid snowy (to break down
red blood cells)
B - 1 ml methyl violet Methyl violet (dye
to white cells).
C - 96 ml of distilled water
3 - covers slides
4 - Microscope
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:The principle of the experiment

Dependent on blood-thinning
experiment the solution to ease your
white blood cells, which analyzes the
red blood cells, white blood cells,
.leaving intact

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Experiment steps
attended the lotion reduce white - 1
blood cells and Swab the tip of a
.little-used finger with 70% EtOH

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drag by sucking the blood is 0.5 - 2

Mottaglt sign up and then completed
a mitigation solution until the mark
11 and Mix a solution of blood
.dilution

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get rid of a few drops of diluted - 3

blood through the bottom hole of the
pipette then put a drop of blood
diluted at the edge of the glass cover
.subject on the slide

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make small screening force to - 4

determine the distribution of white blood
cells as it should be a homogeneous
distribution and no pool, which was found
it means that blood is not withdrawn in
. the right way did not ease as it should

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count the white blood cells use a - 5

Microscope . The count is in four large
boxes located in the corners of the
large box. Note that all the big box
contains 16 small square, which is the
count in 64 small square

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:Method of calculation
Mitigation = 1:20
The size of the small box = 1 / 4 X 1 / 4 X 1 /
10 = 1 / 160 mm 3
Suppose that the number of white blood cells
in 64 small square (4 X 16) = Q
The number of white blood cells per cubic
millimeter of blood is diluted is calculated the
following equation:
The number of white blood cells in mm 3 of
blood = x / 64 X160 X 20 white blood cell /
mm 3 of blood
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