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LB+ Glu
LB+ Glu+ B
LB Pos Cont
M9 Neg Con
LB+Buffer
LB+ Glu
LB+ Glu+ B
LB Pos Cont
M9 Neg Con
0
0.19
0.32
0.21
0.33
0.3
0
0.09
0.16
0.11
0.33
0.3
20
0.21
0.325
0.22
0.325
0.3
20
0.09
0.16
0.12
0.325
0.3
40
0.285
0.53
0.31
0.45
0.24
40
0.13
0.28
0.16
0.45
0.24
60
0.42
0.77
0.46
0.6
0.245
60
0.24
0.47
0.3
0.6
0.245
80
0.5
0.95
0.64
0.7
0.245
80
0.35
0.68
0.43
0.7
0.245
Absorbance
0.4
0.2
0
LB+Buff
LB Pos
0.8
0.8
0.6
0.6
Absorbance
Absorbance
0.4
0.2
0.2
0
0.4
20
40
60
80
Time(minutes)
LB+Buffer
LB+ Glu
LB Pos Control
M9 Neg Control
LB+Buffer
LB Pos Contr
bsorbance
0.8
0.6
0.4
0.2
0
20
40
60
Time(minutes)
LB+Buffer
LB+ Glu
LB Pos Control
M9 Neg Control
80
LB+Buffer
LB+ Glu
LB+ Glu+ B
LB Pos Cont
M9 Neg Con
0
7.2
7.17
7.22
7.08
7
20
7.27
7.12
7.21
7.06
6.98
60
7.25
6.83
7.24
7.01
6.96
LB+Buffer
LB+ Glu
LB+ Glu+ B
LB Pos Cont
M9 Neg Con
0
7.22
7.06
7.21
7.08
7
20
7.25
6.92
7.2
7.06
6.98
60
7.25
6.79
7.23
7.01
6.96
pH
20
Time(minutes
LB+Buffer
LB+ Glu
LB Pos Control
M9 Neg Control
er
Control
pH
6.8
6.6
20
60
Time(minutes)
LB+ Glu
M9 Neg Control
6.4
20
Time(minutes)
LB+ Glu+ Buffer
LB+Buffer
LB+ Glu
LB Pos Control
M9 Neg Control
er
Control
20
60
Time(minutes)
LB+ Glu
M9 Neg Control
LB+Buffer
LB+ Glu
LB+ Glu+ B
LB Pos Cont
M9 Neg Con
0
22.5
24
23
23
25
20
28
33
30.5
25
32
60
34
30
30
23.5
30
LB+Buffer
LB+ Glu
LB+ Glu+ B
LB Pos Cont
M9 Neg Con
0
23.5
24
23
23
25
20
29
32
30
25
32
60
32
30
31
23.5
30
Temperature(C)
20
10
0
20
Time(minutes)
LB+Buffer
LB+ Glu
LB Pos Control
M9 Neg Control
LB
1F:Temperature vs Time(1:21
Temperature(C)
20
60
35
30
25
20
15
10
5
0
20
Time(minutes)
LB+ Glu
M9 Neg Control
Time(minu
LB+Buffer
LB+ Glu
LB Pos Control
M9 Neg Control
trol
20
60
Time(minutes)
LB+ Glu
M9 Neg Control