17/2/2021 PhysioEx Exercise 1 Activity 3

PhysioEx Lab Report

Exercise 1: Cell Transport Mechanisms and Permeability Activity
3: Simulating Osmotic Pressure
Name: Juan González
Date: 17 February 2021
Session ID: session-0f81b777-55e6-fd4b-8f68-9334c8d3d866

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.
1 Which of the following is true of osmosis?
You correctly answered: It is a type of diffusion.
2 Which of the following occurs when a hypertonic solution is added to cells?
You correctly answered: The cells shrink.
3 The variable that affects osmotic pressure is
You correctly answered: the concentration of nondiffusing solutes.
4 The net movement of water would be into the cell in a You
correctly answered: hypotonic solution.

Experiment Results
Predict Questions
1 Predict Question 1: What effect do you think increasing the Na + Cl- concentration will have?
Your answer: decreased osmotic pressure.
2 Predict Question 2: What do you think will be the pressure result of the current experimental conditions?
You did not answer this question.
Stop & Think Questions
1 Why do you think there is no pressure change?
You correctly answered: Sodium is able to diffuse through the pores.

2 Why do you think there was no pressure change?

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You correctly answered: There is no net movement of water because the solute concentrations are the
same.

Experiment Data

Run Solute MWCO Start Pressure Start Pressure Rate

Number Conc. L L (mm Conc. R R (mm (mM/sec
(mM) Hg) (mM) Hg) )
1 Na+ Cl- 20 5.00 170 0.00 0 0.0000
1 Albumin 20 0.00 170 0.00 0 0.0000
1 Glucose 20 0.00 170 0.00 0 0.0000
+ -
2 Na Cl 20 10.00 340 0.00 0 0.0000
2 Albumin 20 0.00 340 0.00 0 0.0000
2 Glucose 20 0.00 340 0.00 0 0.0000
3 Na+ Cl- 100 0.00 136 0.00 0 0.0000
3 Albumin 100 0.00 136 0.00 0 0.0000
3 Glucose 100 8.00 136 0.00 0 0.0000
4 Na+ Cl- 100 0.00 0 0.00 0 0.0000
4 Albumin 100 0.00 0 0.00 0 0.0000
4 Glucose 100 8.00 0 8.00 0 0.0000
5 Na+ Cl- 200 0.00 0 0.00 0 0.0000
5 Albumin 200 0.00 0 0.00 0 0.0000
5 Glucose 200 8.00 0 0.00 0 0.0036
6 Na+ Cl- 200 0.00 153 0.00 0 0.0000
6 Albumin 200 9.00 153 0.00 0 0.0000
6 Glucose 200 0.00 153 10.00 0 0.0044

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.
1 If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic
pressure will
You correctly answered: double.
2 Which membrane did not allow Na+ Cl- to pass through?
You correctly answered: 20 MWCO.

3 When a solute is able to diffuse through a membrane

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You correctly answered: equilibrium is reached.

4 Water diffuses
You correctly answered: toward solutes.

Review Sheet Results

1 Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has
this effect. How well did the results compare with your prediction?
Your answer:
El aumento se debe al tamaño molecular influye en un mayor gasto de energía para permitir la permeación
del líquido a través de la membrana.
2 Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
Your answer:
Tiene de similar que ambas son difusiones de solvente o agua a través de una membrana semipermeable,
una membrana que permite la difusión de ciertos solutos y agua.
La difusión facilitada diferencia en la concentración de una sustancia en el espacio se denomina gradiente
de concentración y, la ósmosis cuenta con diferentes presiones.
3 Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases
milliosmoles.”
Your answer:
Un osmol es igual a 1 mol (6,02x10^23-) de partículas de soluto, sin importar su composición exacta. Una
solución que contenga 1 mol de glucosa en cada litro tiene una concentración
de 1osm/l. Una solución que contenga1 mol de una molécula que se disocia en tres iones, contendrá 3
osm/l. Luego el osmol, es una entidad demasiado grande para expresar la actividad osmótica de los solutos
en los líquidos corporales.
En general se usa el término miliosmol que es igual a 1/1.000 osmoles.
4 The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the
200 MWCO membrane in place. Explain the results. How well did the results compare with your
prediction?
Your answer:
La glusoca tiene a.
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