9/5/23, 10:29 PM PhysioEx Exercise 1 Activity 5

PhysioEx Lab Report

Exercise 1: Cell Transport Mechanisms and Permeability
Activity 5: Simulating Active Transport
Name: Virna Sari
Date: 5 September 2023
Session ID: session-360f5ad9-5911-4584-9c81-72fcd86021dc

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 The movement of sodium and potassium maintained by the Na+-K+ pump

You correctly answered: requires energy and is against a concentration gradient.

2 The sodium-potassium pump is classified as a(n)

You correctly answered: antiporter.

3 The sodium-potassium pump moves _____ sodium ions and _____ potassium ions
simultaneously.

You correctly answered: 3, 2.

4 Solutes that require active transport for movement might be too large to pass or might
be

You correctly answered: lipid insoluble.

Experiment Results
Predict Questions
1 Predict Question 1: What do you think will result from these experimental conditions?

Your answer: No Na+ will be transported.

2 Predict Question 2: Do you think the addition of glucose carriers will affect the transport
of sodium or potassium?

Your answer: No, it will not affect the transport of either ion.

Stop & Think Questions

1 Why did the sodium transport stop before the transport was completed?

You correctly answered: The ATP was depleted.

2 Why was the equilibrium for the solutes reached earlier?

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9/5/23, 10:29 PM PhysioEx Exercise 1 Activity 5

You correctly answered: There were more pumps for transport.

Experiment Data

Run Solute ATP (mM) Start Start Pumps Carriers Rate

Number Conc. L Conc. R (mM/sec)
(mM) (mM)
1 Na+ Cl- 1 9.000 0.000 500 --- 0.0188
1 K+ Cl- 1 0.000 6.000 500 --- 0.0125
1 Glucose --- 0.000 0.000 --- 0 0.0000
2 Na+ Cl- 3 9.000 0.000 500 --- 0.0025
2 K+ Cl- 3 0.000 6.000 500 --- 0.0017
2 Glucose --- 0.000 0.000 --- 0 0.0000
3 Na+ Cl- 3 9.000 0.000 500 --- 0.0000
3 K+ Cl- 3 0.000 0.000 500 --- 0.0000
3 Glucose --- 0.000 0.000 --- 0 0.0000
4 Na+ Cl- 3 9.000 0.000 800 --- 0.0042
4 K+ Cl- 3 0.000 6.000 800 --- 0.0028
4 Glucose --- 0.000 0.000 --- 0 0.0000
5 Na+ Cl- 3 9.000 0.000 800 --- 0.0042
5 K+ Cl- 3 0.000 6.000 800 --- 0.0028
5 Glucose --- 0.000 10.000 --- 400 0.0028#

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 What happened when you increased the amount of ATP dispensed with the same
concentration of sodium and potassium on either side of the membrane?

You correctly answered: More ions were transported.

2 At what concentration of ATP were the sodium and potassium maximally transported?

You correctly answered: 3 mM ATP.

3 What was the effect of adding more Na+-K+ pumps to the simulated cell?

You correctly answered: Transport of the ions was faster.

4 Describe the effect of adding glucose carriers to the sodium and potassium transport.

You correctly answered: There was no change in the transport rate because glucose is
transported independently.

Review Sheet Results

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9/5/23, 10:29 PM PhysioEx Exercise 1 Activity 5

1 Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM
potassium chloride outside the cell, instead of other concentration ratios.

Your answer:

the significance of using 9 mM chloride inside the cell and 6 mM potassium chloride
outside the cell is that the Na/K pump allows for 3:2 ratio. For every three sodium ions
that go out of the cell, two potassium ions come into the cell.

2 Explain why there was no sodium transport even though ATP was present. How well did
the results compare with your prediction?

Your answer:

Both sodium and potassium are required to move the ions using the pump. if one of the
two are absent then no movement will occur. Potassium needs to be present in order for
sodium to be transport.

3 Explain why the addition of glucose carriers had no effect on sodium or potassium
transport. How well did the results compare with your prediction?

Your answer:

This is because the glucose carries behave independently from the sodium and
potassium pumps. The results compared well with the prediction because the rates of
the sodium and potassium pumps were the same before and after the addition of the
glucose carries.

4 Do you think glucose is being actively transported or transported by facilitated diffusion

in this experiment? Explain your answer.

Your answer:

Glucose is being transported by facilitated diffusion because the actual process of

glucose transport is done without ATP and needs only a transport protein. Glucose
transport is coupled with sodium potassium pump transport of ions to create the
gradient that powers the transportation of glucose.

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