PhysioEx Exercise 1 Activity 1 5/10/22, 13:12

PhysioEx Lab Report

Exercise 1: Cell Transport Mechanisms and Permeability
Activity 1: Simulating Dialysis (Simple Diffusion)
Name: Leire Delgado
Date: 5 October 2022
Session ID: session-552ed6ce-bd93-38dc-671c-3ae441d58199

Pre-lab Quiz Results

You have not completed the Pre-lab Quiz.

Experiment Results
Predict Questions
1 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will
diffuse through the 20 MWCO membrane?

Your answer: No, not at all.

2 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with

607 amino acids, and the average molecular weight of a single amino acid is 135 g/mol.

Which of the following will be able to diffuse through the 200 MWCO membrane?

Your answer: both glucose and albumin.

Stop & Think Questions

1 The reason sodium chloride didn't diffuse left to right is that

You correctly answered: the membrane pore size was too small.

2 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average
molecular weight of a single amino acid is 135 g/mol. There is no reason to run these
solutes at the 20 MWCO because

You correctly answered: glucose and albumin are both too large to pass.

3 The rate of diffusion for urea

You correctly answered: is slower than that for sodium because urea is a larger
i

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PhysioEx Exercise 1 Activity 1 5/10/22, 13:12

molecule.

Experiment Data
Solute MWCO Left Solute Right Solute Average
(mM) (mM) Diffusion
(mM/sec)
Na+ Cl- 20 9.00 0.00 0.0000
Urea 20 0.00 0.00 0.0000
Albumin 20 0.00 0.00 0.0000
Glucose 20 0.00 0.00 0.0000
Na+ Cl- 20 0.00 0.00 0.0000
Urea 20 9.00 0.00 0.0000
Albumin 20 0.00 0.00 0.0000
Glucose 20 0.00 0.00 0.0000
Na+ Cl- 50 9.00 0.00 0.0150
Urea 50 0.00 0.00 0.0000
Albumin 50 0.00 0.00 0.0000
Glucose 50 0.00 0.00 0.0000
Na+ Cl- 50 18.00 0.00 0.0273
Urea 50 0.00 0.00 0.0000
Albumin 50 0.00 0.00 0.0000
Glucose 50 0.00 0.00 0.0000
Na+ Cl- 100 9.00 0.00 0.0150
Urea 100 0.00 0.00 0.0000
Albumin 100 0.00 0.00 0.0000
Glucose 100 0.00 0.00 0.0000
Na+ Cl- 100 0.00 0.00 0.0000
Urea 100 9.00 0.00 0.0094
Albumin 100 0.00 0.00 0.0000
Glucose 100 0.00 0.00 0.0000
Na+ Cl- 200 0.00 0.00 0.0000
Urea 200 0.00 0.00 0.0000
Albumin 200 0.00 0.00 0.0000
Glucose 200 9.00 0.00 0.0042
Na+ Cl- 200 0.00 0.00 0.0000

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PhysioEx Exercise 1 Activity 1 5/10/22, 13:12

Urea 200 0.00 0.00 0.0000

Albumin 200 9.00 0.00 0.0000
Glucose 200 0.00 0.00 0.0000

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in

the left beaker was to

You correctly answered: increase the rate of diffusion.

2 Describe the difference between the rate of diffusion seen for sodium and urea.

You correctly answered: Urea diffused more slowly because it is larger than sodium.

3 Which of the following solutes did not pass through any of the membranes?

You correctly answered: albumin.

4 When diffusion stops, we say the solution has reached

You correctly answered: equilibrium.

Review Sheet Results

1 Describe two variables that affect the rate of diffusion.

Your answer:

Dos de las variables que afectan a la difusión son la concentración de la disolución y el

peso molecular.

2 Why do you think the urea was not able to diffuse through the 20 MWCO membrane?
How well did the results compare with your prediction?

Your answer:

El peso de la urea es de unos 60 g/mol y el de la membrana es de 20 g/mol. La

molécula de urea es más grande que los poros de la membrana, a través de los cuales
no puede pasar por lo cual la difusión no puede producirse.

3 Describe the results of the attempts to diffuse glucose and albumin through the 200

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PhysioEx Exercise 1 Activity 1 5/10/22, 13:12

MWCO membrane. How well did the results compare with your prediction?

Your answer:

La glucosa con una velocidad promedio de 0,02 mm/s cruza la membrana para
alcanzar el equilibrio entre las dos soluciones. En cambio, en la solución de la
albúmina, dado que su peso molecular es alto, no hay difusión.

4 Put the following in order from smallest to largest molecular weight: glucose, sodium
chloride, albumin, and urea.

Your answer:

Cloruro sódico es la molécula con menor peso molecular. Luego, la molécula con
menos peso es la urea. La siguiente molécula es la glucosa. Por último, la molécula
más grande es la albúmina.

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