Name: Fernanda, Shantal, k

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1. Which of the following is true of osmosis?

You correctly answered: c. It is a type of diffusion.

2. Which of the following occurs when a hypertonic solution is added to cells?

You correctly answered: d. The cells shrink.

3. The variable that affects osmotic pressure is

You correctly answered: a. the concentration of nondiffusing solutes.

4. The net movement of water would be into the cell in a

You correctly answered: b. hypotonic solution.

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Experiment Results
Predict Question:
Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have?
Your answer : a. increased osmotic pressure

Predict Question 2: What do you think will be the pressure result of the current experimental conditions?
Your answer : c. pressure above the left beaker

Stop & Think Questions:

Why do you think there is no pressure change?
You correctly answered: b. Sodium is able to diffuse through the pores.

Why do you think there was no pressure change?

You correctly answered: c. There is no net movement of water because the solute concentrations are the same.

Experiment Data:

Run Number Solute MWCO Start Conc. L Pres. L Start Conc. R Pres. R Rate
1 Na+ Cl- 20 5.00 170 0.00 0 0.0000
1 Albumin 20 0.00 170 0.00 0 0.0000
1 Glucose 20 0.00 170 0.00 0 0.0000
2 Na+ Cl- 20 10.00 340 0.00 0 0.0000
2 Albumin 20 0.00 340 0.00 0 0.0000
2 Glucose 20 0.00 340 0.00 0 0.0000
3 Na+ Cl- 50 10.00 0 0.00 0 0.0167
3 Albumin 50 0.00 0 0.00 0 0.0000
3 Glucose 50 0.00 0 0.00 0 0.0000
4 Na+ Cl- 100 0.00 136 0.00 0 0.0000
4 Albumin 100 0.00 136 0.00 0 0.0000
4 Glucose 100 8.00 136 0.00 0 0.0000
5 Na+ Cl- 100 0.00 0 0.00 0 0.0000
5 Albumin 100 0.00 0 0.00 0 0.0000
5 Glucose 100 8.00 0 8.00 0 0.0000
6 Na+ Cl- 200 0.00 0 0.00 0 0.0000
6 Albumin 200 0.00 0 0.00 0 0.0000
6 Glucose 200 8.00 0 0.00 0 0.0036
7 Na+ Cl- 200 0.00 153 0.00 0 0.0000
7 Albumin 200 9.00 153 0.00 0 0.0000
7 Glucose 200 0.00 153 10.00 0 0.0044

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will
You correctly answered: c. double.

2. Which membrane did not allow Na+ Cl- to pass through?

You correctly answered: a. 20 MWCO

3. When a solute is able to diffuse through a membrane

You correctly answered: b. equilibrium is reached.

4. Water diffuses
You correctly answered: c. toward solutes.

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Review Sheet Results
1. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well
did the results compare with your prediction?
Your answer:
Incrementa la presión ósmotica porque el agua pasa a favor del soluto para que exista un equilibrio.

2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
Your answer:
La ósmosis es similar a la difusión simple porque las dos van a favor del gradiente de concentración.
La diferencia es que ósmosis tiene que ver con el solvente y la difusión simple con el soluto.

3. Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.”
Your answer:
El agua siempre va a ir a donde existe más soluto.

4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO
membrane in place. Explain the results. How well did the results compare with your prediction?
Your answer:
Porque la albumina es más pesada entonces no pasa por el poro de la membrana de 200 y por ello el agua va a favor del
lado izquierdo y aumenta la presión ósmotica.

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