31/8/23, 12:45 PhysioEx Exercise 1 Activity 5

PhysioEx Lab Report

Exercise 1: Cell Transport Mechanisms and Permeability
Activity 5: Simulating Active Transport
Name: Claudia Becerra Gonzales
Date: 31 August 2023
Session ID: session-367442fb-4bff-52fa-3ffa-45cb1738cbb3

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 The movement of sodium and potassium maintained by the Na+-K+ pump

You correctly answered: requires energy and is against a concentration gradient.

2 The sodium-potassium pump is classified as a(n)

You correctly answered: antiporter.

3 The sodium-potassium pump moves _____ sodium ions and _____ potassium ions
simultaneously.

You correctly answered: 3, 2.

4 Solutes that require active transport for movement might be too large to pass or might
be

You correctly answered: lipid insoluble.

Experiment Results
Predict Questions
1 Predict Question 1: What do you think will result from these experimental conditions?

Your answer: No Na+ will be transported.

2 Predict Question 2: Do you think the addition of glucose carriers will affect the transport
of sodium or potassium?

Your answer: No, it will not affect the transport of either ion.

Stop & Think Questions

1 Why did the sodium transport stop before the transport was completed?

You correctly answered: The ATP was depleted.

2 Why was the equilibrium for the solutes reached earlier?

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31/8/23, 12:45 PhysioEx Exercise 1 Activity 5

You correctly answered: There were more pumps for transport.

Experiment Data

Run Solute ATP (mM) Start Start Pumps Carriers Rate

Number Conc. L Conc. R (mM/sec)
(mM) (mM)
1 Na+ Cl- 1 9.000 0.000 500 --- 0.0188
1 K+ Cl- 1 0.000 6.000 500 --- 0.0125
1 Glucose --- 0.000 0.000 --- 0 0.0000
2 Na+ Cl- 3 9.000 0.000 500 --- 0.0025
2 K+ Cl- 3 0.000 6.000 500 --- 0.0017
2 Glucose --- 0.000 0.000 --- 0 0.0000
3 Na+ Cl- 3 9.000 0.000 500 --- 0.0000
3 K+ Cl- 3 0.000 0.000 500 --- 0.0000
3 Glucose --- 0.000 0.000 --- 0 0.0000
4 Na+ Cl- 3 9.000 0.000 800 --- 0.0042
4 K+ Cl- 3 0.000 6.000 800 --- 0.0028
4 Glucose --- 0.000 0.000 --- 0 0.0000
5 Na+ Cl- 3 9.000 0.000 800 --- 0.0042
5 K+ Cl- 3 0.000 6.000 800 --- 0.0028
5 Glucose --- 0.000 10.000 --- 400 0.0028#

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 What happened when you increased the amount of ATP dispensed with the same
concentration of sodium and potassium on either side of the membrane?

You correctly answered: More ions were transported.

2 At what concentration of ATP were the sodium and potassium maximally transported?

You correctly answered: 3 mM ATP.

3 What was the effect of adding more Na+-K+ pumps to the simulated cell?

You correctly answered: Transport of the ions was faster.

4 Describe the effect of adding glucose carriers to the sodium and potassium transport.

You correctly answered: There was no change in the transport rate because glucose is
transported independently.

Review Sheet Results

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31/8/23, 12:45 PhysioEx Exercise 1 Activity 5

1 Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM
potassium chloride outside the cell, instead of other concentration ratios.

Your answer:

Es importante que el cloruro de sodio sea 9 y el cloruro de potasio sea 6 debido que la
proporción de sodio y potasio es de 3 a 2 respectivamente para llegar a equilibrio
osmótico debido a sus diferentes potenciales de acción.

2 Explain why there was no sodium transport even though ATP was present. How well did
the results compare with your prediction?

Your answer:

No se transportó cloruro de sodio debido principalmente a que la bomba de sodio y

potasio funcionan con las diferencias de potencial de acción de ambos iones, sin el
cloruro de potasio el cloruro de sodio no podrá ser transportado.

3 Explain why the addition of glucose carriers had no effect on sodium or potassium
transport. How well did the results compare with your prediction?

Your answer:

Se debe principalmente a la forma que se transporta la glucosa por un lado el sodio y

potasio son transportados en contra de la gradiente y por otro lado la glucosa usa la
gradiente concentración para atravesar la membrana celular.

4 Do you think glucose is being actively transported or transported by facilitated diffusion

in this experiment? Explain your answer.

Your answer:

La glucosa se transporta mediante difusión facilitada debido a que usa a favor el

gradiente de concentración.

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