PhysioEx Lab Report

Exercise 1: Cell Transport Mechanisms and Permeability

Activity 3: Simulating Osmotic Pressure
Name:
Date: 1 June 2023
Session ID: session-aed6d02f-c33e-6971-84b8-de216d1ac2cb

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1 Which of the following is true of osmosis?

You correctly answered: It is a type of diffusion.

2 Which of the following occurs when a hypertonic solution is added to cells?

You correctly answered: The cells shrink.

3 The variable that affects osmotic pressure is

You correctly answered: the concentration of nondiffusing solutes.

4 The net movement of water would be into the cell in a

You correctly answered: hypotonic solution.

Experiment Results
Predict Questions
1 Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will
have?

Your answer: increased osmotic pressure.

2 Predict Question 2: What do you think will be the pressure result of the current
experimental conditions?

Your answer: pressure above the left beaker.

Stop & Think Questions

1 Why do you think there is no pressure change?

You correctly answered: Sodium is able to diffuse through the pores.

2 Why do you think there was no pressure change?

 You correctly answered: There is no net movement of water because the solute
concentrations are the same.
Experiment Data

Run Solute MWCO Start Pressure Start Pressure Rate

Number Conc. L L (mm Conc. R R (mm (mM/sec)
(mM) Hg) (mM) Hg)
1 Na+ Cl- 20 5.00 170 0.00 0 0.0000
1 Albumin 20 0.00 170 0.00 0 0.0000
1 Glucose 20 0.00 170 0.00 0 0.0000
2 Na+ Cl- 20 10.00 340 0.00 0 0.0000
2 Albumin 20 0.00 340 0.00 0 0.0000
2 Glucose 20 0.00 340 0.00 0 0.0000
3 Na+ Cl- 50 10.00 0 0.00 0 0.0167
3 Albumin 50 0.00 0 0.00 0 0.0000
3 Glucose 50 0.00 0 0.00 0 0.0000
4 Na+ Cl- 100 8.00 0 0.00 0 0.0133
4 Albumin 100 0.00 0 0.00 0 0.0000
4 Glucose 100 0.00 0 0.00 0 0.0000
5 Na+ Cl- 100 0.00 0 0.00 0 0.0000
5 Albumin 100 0.00 0 0.00 0 0.0000
5 Glucose 100 8.00 0 8.00 0 0.0000
6 Na+ Cl- 200 0.00 0 0.00 0 0.0000
6 Albumin 200 0.00 0 0.00 0 0.0000
6 Glucose 200 8.00 0 0.00 0 0.0036
7 Na+ Cl- 200 0.00 153 0.00 0 0.0000
7 Albumin 200 9.00 153 0.00 0 0.0000
7 Glucose 200 0.00 153 10.00 0 0.0044

Post-lab Quiz Results

You have not completed the Post-lab Quiz.

Review Sheet Results

1 Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and
why it has this effect. How well did the results compare with your prediction?

You did not answer this question.

2 Describe one way in which osmosis is similar to simple diffusion and one way in which it
is different.
 You did not answer this question.

3 Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases
milliosmoles.”

You did not answer this question.

4 The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right
beaker with the 200 MWCO membrane in place. Explain the results. How well did the
results compare with your prediction?

You did not answer this question.