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First Name
Last Name
Dinesh
Dinesh
Your Age
14
Last Name
Your Age
Age
First Name
Last Name
Your Age
Age
First Name
Your Age
Age
Last Name
Curriculum
British
City
99500
Dubai
Emirate
Country
Dubai
UnitedArabEmirates
PO Box
City
52232
Dubai
Emirate
Country
Dubai
UnitedArabEmirates
First Name
MuhammadAnwar
Last Name
UlHaq
Email
muhammad_ulhaq74@yahoo.com
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ToexplorewhetherornottheabsenceofgravityatISSaffectsPCRinDNA
Replication,specificallytheenzymereactionofDNAPolymeraseI
IbelievethatthemicrogravityonboardattheISSwillmutatetheDNAin
somewayduetoitsreadaptationinalmostzerogravityandthechangeof
itsrateofreactionintheenzymeactivityofDNAPolymeraseI,whichisthe
maincomponentinbindingtheDNA.Ihavetofindoutifmytheoretical
predictionsaretrueandiftheyapplytothepracticalworld.Reachingan
anticipatedconclusionwillmaybehelpusrelatetosomeconditionsthat
astronautsandspacetravelersfaceafterreturningbackingtoEartheg.Bone
densitydepletionetc.Ifwereabletodecipherthecausesbehindthese
issues,wemightbeabletolateravoidthemusingcountermeasure
equipment/precautions.
5. Outline your experimental plan. How will you use PCR to test
your hypothesis? Specify for example: the samples you will
analyze, controls that you will use, and the possible
experimental outcomes.
PCRwillyouusedalongwithatarget,withtheDNAcontainingthegene
interestedinalongwiththetwoprimersalongwiththeenzymeDNAPolymerase
I,foundinalllivingcellsandresponsibleforcopyingDNAwhencell
dividesandchemicalsthatPolymeraseneedstobuildnewDNA.Temperatureis
controlledandisfirstheatedusingspecialPolymerasetoseparatethe
strands,whichwoulddestroynormalenzymes.Later,itiscooledtofindthe
matchinggeneandthenisleftattheoptimumtemperature.Theforceof
gravity,moisturelevels,radiationexposureandallotherfactorswhich
couldcontaminatetheexperimentshouldbecontrolledandanyformof
fluctuationcoulddecreasetheaccuracyoftheexperiment.Outcomesinclude
mutationsintheDNAduetotheeffectsofgravityandchangeintherateof
reactionandreplicationinDNA,eitherslowerorfasterandexperimentcan
bereplicatedtoprovidemoreaccurateoutcomes.