Site

Secretion

Enzyme

Substrate

End
Product

Optimum
Conditions

Tub
e
1
2
3

1%
starch
solution
5.0 ml
5.0 ml
5.0 ml

4
5
6

Tub
e
1

1.0 M
HCl

1.0 M
H3PO4

1.0 M
NaCl

Work-up

3 ml
-

0.2%
amylase
solution*
3 ml
3 ml

5.0 ml
5.0 ml

3 ml
3 ml

10 drops
-

5.0 ml

3 ml

10
drops
-

Heat in boiling
H2O bath, 5
mins.
-

H 2O

10
drops
-

3
4
5

5 ml
-

H2O

2% neutral
pepsin solution
5 ml

1.0 M
HCl
-

1.0 M
NaOH
-

5 ml

10 drops

5 ml
5 ml

10 drops
10 drops
-

10
drops

10
drops

Work-up

Place in ice
bath, 5 mins.

Tub
e
1
2
3
4
5

H2O

5 ml
-

2% neutral
pancreatin
solution
5 ml
5 ml
5 ml
5 ml

Bile salt

1.0 M CaCl2

0.1
0.1
0.1
0.1

10 drops
-

g
g
g
g

1.0 M
Na2CO3
10 drops