S25 HPLC s25

Time(min) Absorbance Concentration conc 183.047ppm

0.0 0.8090 0.3804
0.5 0.7280 0.3420
1.0 0.6580 0.3092
1.5 0.5700 0.2681
2.0 0.5280 0.2480
2.5 0.4730 0.2225
3.0 0.4130 0.2943
3.5 0.3920 0.1844

S60
Time(min) Absorbance Concentration
0.0 0.9560 0.4492
0.5 0.8090 0.3804
1.0 0.6630 0.3116
1.5 0.6180 0.2903
2.0 0.5580 0.2623
2.5 0.4490 0.2111
3.0 0.4330 0.2037
3.5 0.4300 0.2033

finding specific binding constant

concentration of starchAbsorbance Concentration
0.4 0.1464 0.3110
1.0 0.3084 0.6560
1.5 0.7223 1.5370
2.0 1.0328 2.1970
3.0 1.1994 2.5520
s60
176.370ppm