Effectiveness of a 2%

chlorhexidine solution mixed

with antifungal agent and
calcium hydroxide against
Candida albicans
SUBMITTED TO PROF.RODRIGUEZ, SOCORROM

Dr.O.R.Ganesamurthi
Olappalayam Rathinam Ganesamurthi
2 year 2 semester
FREQUENCIES VARIABLES=GROUP COLONY
/ORDER=ANALYSIS.

Frequencies
Notes
Output Created 10-Feb-2011 18:54:24
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File <none>
N of Rows in Working 60
Data File
Missing Value Definition of Missing User-defined missing values
Handling are treated as missing.
Cases Used Statistics are based on all
cases with valid data.
Syntax FREQUENCIES
VARIABLES=GROUP
COLONY
/ORDER=ANALYSIS.

Resources Processor Time 00 00:00:00.032

Elapsed Time 00 00:00:00.079

[DataSet0]

Statistics
GROUP COLONY
N Valid 60 60
Missing 0 0
Frequency Table

GROUP
Frequenc Valid Cumulative
y Percent Percent Percent
Valid 1.00 20 33.3 33.3 33.3
2.00 20 33.3 33.3 66.7
3.00 20 33.3 33.3 100.0
Total 60 100.0 100.0

COLONY
Frequenc Valid Cumulative
y Percent Percent Percent
Valid 123.00 1 1.7 1.7 1.7
156.00 1 1.7 1.7 3.3
198.00 2 3.3 3.3 6.7
213.00 1 1.7 1.7 8.3
234.00 2 3.3 3.3 11.7
243.00 1 1.7 1.7 13.3
287.00 1 1.7 1.7 15.0
289.00 1 1.7 1.7 16.7
321.00 2 3.3 3.3 20.0
324.00 2 3.3 3.3 23.3
342.00 1 1.7 1.7 25.0
356.00 1 1.7 1.7 26.7
365.00 1 1.7 1.7 28.3
376.00 1 1.7 1.7 30.0
421.00 1 1.7 1.7 31.7
432.00 3 5.0 5.0 36.7
453.00 1 1.7 1.7 38.3
467.00 1 1.7 1.7 40.0
487.00 1 1.7 1.7 41.7
498.00 1 1.7 1.7 43.3
532.00 1 1.7 1.7 45.0
546.00 1 1.7 1.7 46.7
554.00 1 1.7 1.7 48.3
567.00 5 8.3 8.3 56.7
568.00 1 1.7 1.7 58.3
573.00 1 1.7 1.7 60.0
587.00 1 1.7 1.7 61.7
611.00 1 1.7 1.7 63.3
634.00 1 1.7 1.7 65.0
654.00 1 1.7 1.7 66.7
657.00 1 1.7 1.7 68.3
671.00 2 3.3 3.3 71.7
678.00 1 1.7 1.7 73.3
687.00 1 1.7 1.7 75.0
698.00 1 1.7 1.7 76.7
721.00 2 3.3 3.3 80.0
732.00 1 1.7 1.7 81.7
763.00 1 1.7 1.7 83.3
765.00 1 1.7 1.7 85.0
783.00 1 1.7 1.7 86.7
786.00 1 1.7 1.7 88.3
832.00 1 1.7 1.7 90.0
874.00 1 1.7 1.7 91.7
875.00 1 1.7 1.7 93.3
876.00 1 1.7 1.7 95.0
879.00 1 1.7 1.7 96.7
911.00 1 1.7 1.7 98.3
976.00 1 1.7 1.7 100.0
Total 60 100.0 100.0
CROSSTABS
/TABLES=GROUP BY COLONY
/FORMAT=AVALUE TABLES
/STATISTICS=CHISQ
/CELLS=COUNT
/COUNT ROUND CELL
/BARCHART.

Crosstabs

Notes
Output Created 10-Feb-2011 18:56:41
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File <none>
N of Rows in Working 60
Data File
Missing Value Definition of Missing User-defined missing values
Handling are treated as missing.
Cases Used Statistics for each table are
based on all the cases with
valid data in the specified
range(s) for all variables in
each table.
Syntax CROSSTABS
/TABLES=GROUP BY
COLONY
/FORMAT=AVALUE TABLES
/STATISTICS=CHISQ
/CELLS=COUNT
/COUNT ROUND CELL
/BARCHART.

Resources Processor Time 00 00:00:02.106

Elapsed Time 00 00:00:01.840
Dimensions Requested 2
Cells Available 174762

[DataSet0]

Case Processing Summary

Cases
Valid Missing Total
N Percent N Percent N Percent
GROUP * 60 100.0% 0 .0% 60 100.0%
COLONY
 Chi-Square Tests
Asymp. Sig.
Value df (2-sided)
Pearson Chi-Square 105.800a 94 .191
Likelihood Ratio 118.512 94 .045
Linear-by-Linear 43.683 1 .000
Association
N of Valid Cases 60
a. 144 cells (100.0%) have expected count less than 5.
The minimum expected count is .33.

FREQUENCIES VARIABLES=COLONY
/HISTOGRAM NORMAL
 /ORDER=ANALYSIS.

Frequencies

Notes
Output Created 10-Feb-2011 19:00:51
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File <none>
N of Rows in Working 60
Data File
Missing Value Definition of Missing User-defined missing values
Handling are treated as missing.
Cases Used Statistics are based on all
cases with valid data.
Syntax FREQUENCIES
VARIABLES=COLONY
/HISTOGRAM NORMAL
/ORDER=ANALYSIS.

Resources Processor Time 00 00:00:00.328

Elapsed Time 00 00:00:00.375
[DataSet0]

Statistics
COLONY
N Valid 60
Missing 0

COLONY
Frequenc Valid Cumulative
y Percent Percent Percent
Valid 123.00 1 1.7 1.7 1.7
156.00 1 1.7 1.7 3.3
198.00 2 3.3 3.3 6.7
213.00 1 1.7 1.7 8.3
234.00 2 3.3 3.3 11.7
243.00 1 1.7 1.7 13.3
287.00 1 1.7 1.7 15.0
289.00 1 1.7 1.7 16.7
321.00 2 3.3 3.3 20.0
324.00 2 3.3 3.3 23.3
342.00 1 1.7 1.7 25.0
356.00 1 1.7 1.7 26.7
365.00 1 1.7 1.7 28.3
376.00 1 1.7 1.7 30.0
421.00 1 1.7 1.7 31.7
432.00 3 5.0 5.0 36.7
453.00 1 1.7 1.7 38.3
467.00 1 1.7 1.7 40.0
487.00 1 1.7 1.7 41.7
498.00 1 1.7 1.7 43.3
532.00 1 1.7 1.7 45.0
546.00 1 1.7 1.7 46.7
554.00 1 1.7 1.7 48.3
567.00 5 8.3 8.3 56.7
568.00 1 1.7 1.7 58.3
573.00 1 1.7 1.7 60.0
587.00 1 1.7 1.7 61.7
611.00 1 1.7 1.7 63.3
634.00 1 1.7 1.7 65.0
654.00 1 1.7 1.7 66.7
657.00 1 1.7 1.7 68.3
671.00 2 3.3 3.3 71.7
678.00 1 1.7 1.7 73.3
687.00 1 1.7 1.7 75.0
698.00 1 1.7 1.7 76.7
721.00 2 3.3 3.3 80.0
732.00 1 1.7 1.7 81.7
763.00 1 1.7 1.7 83.3
765.00 1 1.7 1.7 85.0
783.00 1 1.7 1.7 86.7
786.00 1 1.7 1.7 88.3
832.00 1 1.7 1.7 90.0
874.00 1 1.7 1.7 91.7
875.00 1 1.7 1.7 93.3
876.00 1 1.7 1.7 95.0
879.00 1 1.7 1.7 96.7
911.00 1 1.7 1.7 98.3
976.00 1 1.7 1.7 100.0
Total 60 100.0 100.0
*Nonparametric Tests: Independent Samples.
NPTESTS
/INDEPENDENT TEST (COLONY) GROUP (GROUP)
MEDIAN(TESTVALUE=SAMPLE COMPARE=PAIRWISE)
/MISSING SCOPE=ANALYSIS USERMISSING=EXCLUDE
/CRITERIA ALPHA=0.05 CILEVEL=95.

Nonparametric Tests
 Notes
Output Created 10-Feb-2011 19:03:46
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File <none>
N of Rows in Working 60
Data File
Syntax NPTESTS
/INDEPENDENT TEST
(COLONY) GROUP (GROUP)
MEDIAN(TESTVALUE=SAMPL
E COMPARE=PAIRWISE)
/MISSING
SCOPE=ANALYSIS
USERMISSING=EXCLUDE
/CRITERIA ALPHA=0.05
CILEVEL=95.

Resources Processor Time 00 00:00:00.640

Elapsed Time 00 00:00:01.061

[DataSet0]
SORT CASES BY GROUP.
SPLIT FILE LAYERED BY GROUP.
DESCRIPTIVES VARIABLES=COLONY
/STATISTICS=MEAN STDDEV MIN MAX.

Descriptives

Notes
Output Created 10-Feb-2011 19:07:09
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File GROUP
N of Rows in Working 60
Data File
Missing Value Definition of Missing User defined missing values
Handling are treated as missing.
Cases Used All non-missing data are used.
Syntax DESCRIPTIVES
VARIABLES=COLONY
/STATISTICS=MEAN
STDDEV MIN MAX.

Resources Processor Time 00 00:00:00.016

Elapsed Time 00 00:00:00.014

[DataSet0]
 Descriptive Statistics
Std.
GROUP N Minimum Maximum Mean Deviation
1.00 COLONY 20 567.00 976.00 754.8500 123.04996
Valid N 20
(listwise)
2.00 COLONY 20 365.00 721.00 553.2500 99.97414
Valid N 20
(listwise)
3.00 COLONY 20 123.00 611.00 300.7000 111.67674
Valid N 20
(listwise)

FREQUENCIES VARIABLES=COLONY
/HISTOGRAM NORMAL
/ORDER=ANALYSIS.

Frequencies

Notes
Output Created 10-Feb-2011 19:08:14
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File GROUP
N of Rows in Working 60
Data File
Missing Value Definition of Missing User-defined missing values
Handling are treated as missing.
Cases Used Statistics are based on all
cases with valid data.
Syntax FREQUENCIES
VARIABLES=COLONY
/HISTOGRAM NORMAL
/ORDER=ANALYSIS.

Resources Processor Time 00 00:00:00.873

Elapsed Time 00 00:00:00.858

[DataSet0]

Statistics
COLONY
1.00 N Valid 20
Missing 0
2.00 N Valid 20
Missing 0
3.00 N Valid 20
Missing 0

COLONY
Frequenc Valid Cumulative
GROUP y Percent Percent Percent
1.00 Valid 567.00 3 15.0 15.0 15.0
634.00 1 5.0 5.0 20.0
654.00 1 5.0 5.0 25.0
657.00 1 5.0 5.0 30.0
678.00 1 5.0 5.0 35.0
721.00 1 5.0 5.0 40.0
732.00 1 5.0 5.0 45.0
763.00 1 5.0 5.0 50.0
765.00 1 5.0 5.0 55.0
783.00 1 5.0 5.0 60.0
 786.00 1 5.0 5.0 65.0
832.00 1 5.0 5.0 70.0
874.00 1 5.0 5.0 75.0
875.00 1 5.0 5.0 80.0
876.00 1 5.0 5.0 85.0
879.00 1 5.0 5.0 90.0
911.00 1 5.0 5.0 95.0
976.00 1 5.0 5.0 100.0
Total 20 100.0 100.0
2.00 Valid 365.00 1 5.0 5.0 5.0
421.00 1 5.0 5.0 10.0
432.00 1 5.0 5.0 15.0
453.00 1 5.0 5.0 20.0
467.00 1 5.0 5.0 25.0
487.00 1 5.0 5.0 30.0
498.00 1 5.0 5.0 35.0
532.00 1 5.0 5.0 40.0
546.00 1 5.0 5.0 45.0
554.00 1 5.0 5.0 50.0
567.00 2 10.0 10.0 60.0
568.00 1 5.0 5.0 65.0
573.00 1 5.0 5.0 70.0
587.00 1 5.0 5.0 75.0
671.00 2 10.0 10.0 85.0
687.00 1 5.0 5.0 90.0
698.00 1 5.0 5.0 95.0
721.00 1 5.0 5.0 100.0
Total 20 100.0 100.0
3.00 Valid 123.00 1 5.0 5.0 5.0
156.00 1 5.0 5.0 10.0
198.00 2 10.0 10.0 20.0
213.00 1 5.0 5.0 25.0
234.00 2 10.0 10.0 35.0
243.00 1 5.0 5.0 40.0
287.00 1 5.0 5.0 45.0
289.00 1 5.0 5.0 50.0
 321.00 2 10.0 10.0 60.0
324.00 2 10.0 10.0 70.0
342.00 1 5.0 5.0 75.0
356.00 1 5.0 5.0 80.0
376.00 1 5.0 5.0 85.0
432.00 2 10.0 10.0 95.0
611.00 1 5.0 5.0 100.0
Total 20 100.0 100.0

Histogram
SPLIT FILE OFF.
NPAR TESTS
/K-W=COLONY BY GROUP(1 3)
/MISSING ANALYSIS.

NPar Tests
 Notes
Output Created 10-Feb-2011 19:12:50
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File <none>
N of Rows in Working 60
Data File
Missing Value Definition of Missing User-defined missing values
Handling are treated as missing.
Cases Used Statistics for each test are
based on all cases with valid
data for the variable(s) used in
that test.
Syntax NPAR TESTS
/K-W=COLONY BY GROUP(1
3)
/MISSING ANALYSIS.

Resources Processor Time 00 00:00:00.016

Elapsed Time 00 00:00:00.016
Number of Cases 112347
Alloweda
a. Based on availability of workspace memory.

[DataSet0]

Kruskal-Wallis Test
 Ranks
Mean
GROUP N Rank
COLONY 1.00 20 48.08
2.00 20 31.73
3.00 20 11.70
Total 60

Test Statisticsa,b
COLONY
Chi-Square 43.566
df 2
Asymp. .000
Sig.
a. Kruskal Wallis Test
b. Grouping Variable:
GROUP

NPAR TESTS
/M-W= COLONY BY GROUP(1 2)
/MISSING ANALYSIS.

NPar Tests

Notes
Output Created 10-Feb-2011 19:26:00
Comments
Input Active Dataset DataSet0
Filter <none>
 Weight <none>
Split File <none>
N of Rows in Working 60
Data File
Missing Value Definition of Missing User-defined missing values
Handling are treated as missing.
Cases Used Statistics for each test are
based on all cases with valid
data for the variable(s) used in
that test.
Syntax NPAR TESTS
/M-W= COLONY BY
GROUP(1 2)
/MISSING ANALYSIS.

Resources Processor Time 00 00:00:00.015

Elapsed Time 00 00:00:00.110
Number of Cases 112347
Alloweda
a. Based on availability of workspace memory.

[DataSet0]

Mann-Whitney Test

Ranks
Mean Sum of
GROUP N Rank Ranks
COLONY 1.00 20 28.23 564.50
2.00 20 12.78 255.50
Total 40
 Test Statisticsb
COLONY
Mann-Whitney U 45.500
Wilcoxon W 255.500
Z -4.184
Asymp. Sig. (2-tailed) .000
Exact Sig. [2*(1-tailed .000a
Sig.)]
a. Not corrected for ties.
b. Grouping Variable: GROUP

NPAR TESTS
/M-W= COLONY BY GROUP(1 3)
/MISSING ANALYSIS.

NPar Tests

Notes
Output Created 10-Feb-2011 19:27:51
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File <none>
N of Rows in Working 60
Data File
Missing Value Definition of Missing User-defined missing values
Handling are treated as missing.
 Cases Used Statistics for each test are
based on all cases with valid
data for the variable(s) used in
that test.
Syntax NPAR TESTS
/M-W= COLONY BY
GROUP(1 3)
/MISSING ANALYSIS.

Resources Processor Time 00 00:00:00.000

Elapsed Time 00 00:00:00.000
Number of Cases 112347
Alloweda
a. Based on availability of workspace memory.

[DataSet0]

Mann-Whitney Test

Ranks
Mean Sum of
GROUP N Rank Ranks
COLONY 1.00 20 30.35 607.00
3.00 20 10.65 213.00
Total 40
 Test Statisticsb
COLONY
Mann-Whitney U 3.000
Wilcoxon W 213.000
Z -5.331
Asymp. Sig. (2-tailed) .000
Exact Sig. [2*(1-tailed .000a
Sig.)]
a. Not corrected for ties.
b. Grouping Variable: GROUP

NPAR TESTS
/M-W= COLONY BY GROUP(2 3)
/MISSING ANALYSIS.

NPar Tests

Notes
Output Created 10-Feb-2011 19:28:24
Comments
Input Active Dataset DataSet0
Filter <none>
Weight <none>
Split File <none>
N of Rows in Working 60
Data File
Missing Value Definition of Missing User-defined missing values
Handling are treated as missing.
 Cases Used Statistics for each test are
based on all cases with valid
data for the variable(s) used in
that test.
Syntax NPAR TESTS
/M-W= COLONY BY
GROUP(2 3)
/MISSING ANALYSIS.

Resources Processor Time 00 00:00:00.015

Elapsed Time 00 00:00:00.015
Number of Cases 112347
Alloweda
a. Based on availability of workspace memory.

[DataSet0]

Mann-Whitney Test

Ranks
Mean Sum of
GROUP N Rank Ranks
COLONY 2.00 20 29.45 589.00
3.00 20 11.55 231.00
Total 40
 Test Statisticsb
COLONY
Mann-Whitney U 21.000
Wilcoxon W 231.000
Z -4.844
Asymp. Sig. (2-tailed) .000
Exact Sig. [2*(1-tailed .000a
Sig.)]
a. Not corrected for ties.
b. Grouping Variable: GROUP

Interruptions:
Here in this study the curve is not normally distributed so we are using non
parametric test for compere the groups

Nonparametric Tests:
Independent Samples.
NPTESTS
/INDEPENDENT TEST (COLONY) GROUP (GROUP)
MEDIAN(TESTVALUE=SAMPLE COMPARE=PAIRWISE)
/MISSING SCOPE=ANALYSIS USERMISSING=EXCLUDE
/CRITERIA ALPHA=0.05 CILEVEL=95.
Summary of hypothesis also REJECT THE NULL HYPOTHESIS

Result of Kruskal-Wallis Test

there is highly statically significantly
different between the three groups

result of Mann-Whitney Test

there is statically significantly different between group 1 and group 3

result of Mann-Whitney Test

there is statically significantly different between group 1 and group 2

result of Mann-Whitney Test

there is statically significantly different between group 2 and group 3