(B. Sc.

Biochemistry)
Seventh Semester 2006
Full Marks: 100
Subject: Physical Biochemistry Pass Marks: 45
Time: 3 hours
Candidates are required to give their answers in their own words as far as practicable.
The figure in the margin indicates full marks.
Group (A) Long Questions (Any three) ×14 = 42)
(3×
1. Define motif. Describe all symmetry operations found in macromolecular
structure.
2. Describe cooperative and non co-operative models for transitions in
biopolymer.
3. Derive an expression for first order correction to the energy using
perturbation theory.
4. Explain non specific binding to Nucleic acids. Discuss the electrostatic
effects on binding to nucleic acids.
×
1. Discuss the structure of water.
2. Show that entropy change in a reversible process by classical
and statistical calculations lead to same result.
3. Discuss the complexcities in macromolecular structure.
4. Define molecular potential. Derive force function for a dipole- dipole and
van der waal interactions.
5. How x-ray diffraction is used to solve the structure of molecule in single
crystal?
6. Derive relationship between Bragg’s law and Von-Laue conditions.
7. Derive an expression from which molecular weight of macromolecule is
obtained by measuring osmotic pressure.
8. What is active transport? Discuss how sodium- Potassium pump works.
×
1. Define self energy.
2. How many objects are there in a motif?
3. Why is entropy change positive for protein denaturation process?
4. Write the equation which describes the potential energy for the hydrogen
bond interactions.
5. What will be the statistical weights for …aabbaa… and …abaaba… if is
nucleation parameter and s is the statistical weight for each transition
according to zipper model?
6. What is partition function?
7. Why growing a crystal is difficult?
8. How morphology of a crystal is defined?
9. How can molecular weight of soluble macromolecule be determined?
10. What is the relationship of the transit time with thickness and
permeability in passive transport?
11. What is membrane potential?
12. What is double reciprocal plot?