Universal Science College

(B. Sc. Biochemistry)

Fourth Semester 2005
Internal Examination Full Marks: 100
Subject: Biochem 223 (Molecular Biology) Pass Marks: 45
Date: July 24, 2005 Time: 3 hours
Candidates are required to give their answers in their own words as far as practicable. The
figure in the margin indicates full marks.
Group (A) Long Questions (Any three) ×14 = 42)
(3×

1. Describe DNA-replication process in E. coli

2. Define posttranscriptional modification. Describe various types of introns according to
their activities in the processing.
3. Describe the elongation of peptide chain during translation.
4. Discuss the principles of gene regulation. Describe bacterial lac- operon.

Group (B) Short Questions: (Any six) ×7 = 42)

(6×
1. Describe important structural elements of a yeast chromosome.
2. How does a cell repair thymidine lesion?
3. Describe eukaryotic transcription initiation process.
4. Describe structure and gene products of an integrated retroviral genome.
5. Describe briefly how cracking of codon dictionary became possible.
6. How do ubiquitin molecules cause protein degradation?
7. Describe SOS-response in bacteria.
8. How do Salmonella typhi changes its flagellar antigen?

Group (C) Write short notes on: (Any eight) ×8 = 16)

(2×

1. Tonegawa theory
2. Linking number
3. Nucleosome
4. Eukaryotic RNA polymerases
5. Actinomycin D
6. Zidovudine
7. Aminoacid activation
8. Genes within genes
9. Polysomes
10. Significance of gene regulation
11. IPTG
12. Pattern regulatory genes in Drosophila melanogaster
***