Roll No:

Department of Biochemistry
M.Sc. Semester I Examination, Feb-March 2022
Paper Code: MBC-106
Paper Title: Molecular Biology-I

Time: 1.5 Hours Maximum Marks: 37

Attempt all the questions. All parts of a question must be attempted in continuation. Start
answer of a fresh question on a new page. Cite examples and draw labelled diagrams.

Question 1. Fill the banks with appropriate word. [1 × 7 = 7]

1. If dispersive mode of replication is true, number of bands observed after three round
of replication in Ethidium Bromide density gradient centrifugation will be…………..
2. Alleviation of DNA supercoiling is done be ………………….enzyme.
3. Repetitive DNA has ………………..Cot value.
4. If B form of DNA is subjected to low humidity, it will make…………form of DNA.
5. Appearance of consensus sequence at promoter region represents the strongest
promoter. (True/False)
6. At each origin of replication …………… number(s) of replication forks are formed and
at each replication fork ……….. number(s) of enzymes are at work.
7. End replication problem in eukaryotic cells are often resolved by …………..

Question 2 Attempt ANY THREE of the following. [3x5=15].

(a) Role of sigma factors
(b) Termination of Replication in Escherichia coli
(c) Cap addition and polyadenylation
(d) Prokaryotic promoters
(e) Termination of transcription
Question 3 Attempt ANY ONE of the following. [1x5=15].

(A) Describe the splicing mechanism and emphasise the role of snRNA. Explain the
assembly of spliceosome and release of lariat. Also describe the steps involved in
tRNA processing.
OR
(B) Explain the structure and function of oriC in E. coli replication. Compare it with
Origin recognition complex or Eukaryotes. What are different eukaryotic DNA
polymerase, group them in functional clusters?
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