ONLINE MODE

M.Sc. SEMESTER III ONLINE EXAMINATION, 2021

BIOTECHNOLOGY
Paper Code– MBT CC301
PAPER TITLE: ANIMAL BIOTECHNOLOGY
Time: Three Hours Maximum Marks: 75
Attempt all questions. All parts of a question must be attempted in
continuation. Start answer of a fresh question on a new page. Cite examples
and draw labelled diagrams.
Question No. 1: [10 × 1.5 = 15]
Short notes- answer in two or three sentences:
a. Confluence
b. Cell strain
c. Air-Lift Fermenter culture
d. Inclusion bodies
e. Yeast 2 hybrid
f. Helper virus
g. Cos cells
h. 2-μm plasmid and its importance
i. His tag
j. BSL 3
Question No. 2:
Answer any TWO questions. [2 × 7½ = 15]
A. What are organoid cultures? Discuss the advantages and potential
applications.
B. Give an account of the culture systems developed for scale-up of monolayer
and suspension cultures.
C. Differentiate between a cloning vector and an expression vector. Discuss the
factors that can affect high level expression of a cloned gene.
D. What are fusion proteins? What are the benefits of expressing a recombinant
protein as fusion protein? Explain by giving suitable examples.
Question No. 3:
Answer any TWO questions. [2 × 7½ = 15]
A. Discuss the principle of designing pIN series of expression vectors.
B. How targeting protein to the periplasmic space is advantageous over
intracellular production? Discuss the signal peptides of Gram –ve bacteria as
secretary signals.
C. Give the detailed comparative account of yeast shuttle vectors.
D. What is a promoter? Explain natural and hybrid promoters with an example
of each.
Question No. 4:
Answer any TWO questions. [2 × 7½ = 15]
A. What is the significance of polyhedrin promoter in the expression of a foreign
gene? How the polyhedrin gene is replaced in the baculovirus genome with
the gene of interest?
B. How the SV40 derived vectors are different from the episomal expression
vectors? Discuss in detail the promoters of BPV, EBV and BKV.
C. Give a detailed account of adenovirus and retrovirus based vectors.
D. What is the sight of replication of vaccinia virus? Discuss the applications of
vaccinia virus and pros and cons of its use.
Question No. 5:
Answer any TWO questions. [2 × 7½ = 15]
A. What are the features of Rabbit Reticulocyte lysate as protein synthesis
system? Discuss different components of this system for optimization of
mRNA translation.
B. What are the key features of the expression vector/cassette for the optimal
isolation of recombinant protein?
C. What are the strategies for proteome analysis? Give a detailed account of any
one of them.
D. Discuss the commercial production of human insulin by recombinant DNA
technology.

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It should be noted that 60 minutes extra time will be provided to scan your
answer sheets, make a PDF and mail it to
Email: biotechnologyexam2020@gmail.com