PIPETTE PERFORMANCE VERIFICATION

PIPETTE

OWNER

Test methods conform to DIN 12650-6 and ISO 8655-6

Gravimetric Test Methods for Piston-Operated Volumetric Instruments

LAST NAME

FIRST NAME
Dennis

MODEL

TEST
VOLUMES

SERIAL NUMBER

K26901K

CONDITIONS
RAW DATA

LEAKAGE TEST
CLEANING
SEAL REPLACED
O-RING REPLACED

MILLIGRAMS
50
100
0.928
5.004
1.021
5.004
1.067
4.904
1.226
5.177
1.077
5.069
1.105
4.872
1.078
4.941
0.959
5.095
1.056
4.989
1.096
5.049

1
0.8
0.8

0.4
0.25
0.15

BALANCE TOLERANCES
Max Capacity (grams)
Readability (uG)
Eccentricity (uG)
Repeatability (uG)

0.999699634

Sample #
1
2
3
4
5
6
7
8
9
10

PRECISION
PERCENT + / -

BALANCE

1-May-12
10

TECHNICIAN

INSPECTION

ACCURACY
PERCENT + / -

50
100
200

Date of Manufacture: September 1992. Last Calibration: 2/03

DATE
TEMP (DEG C)
WATER DENSITY
CONVERSION (G / ML)

Chemistry
Parsons Hall
2-2520

DEPARTMENT:
ADDRESS:
TELEPHONE:

200
9.950
9.875
9.848
9.803
9.839
9.675
9.661
9.164
9.622
10.063

5.1
100
3
0.8

MICROLITERS
50
100
200
0.927721 5.00249697 9.947011
1.020693 5.00249697 9.872034
1.06668 4.90252701 9.845042
1.225632 5.17544501 9.800056
1.076677 5.06747745 9.836045
1.104668 4.87053662 9.672094
1.077676 4.93951589 9.658098
0.958712 5.09346964 9.161247
1.055683 4.98750148 9.61911
1.095671 5.04748345 10.05998

ACCURACY, MEAN ERROR, RESOLUTION

TEST
VOLUME
50
AVG
1.1
STD
0.0821

1.4
1.2
1
0.8
0.6
0.4
0.2
0

1.31

0.81

20.00%

UCL, 1.00%

UCL, 0.80%

UCL, 0.80%

LCL, 50
-1.00%

LCL,100
-0.80%

LCL,200
-0.80%

0.00%

-20.00%
-40.00%
-60.00%

TEST RESULTS

-80.00%

-94.99%

5.4

TEST
VOLUME
100
AVG
5.0
STD
0.0917

5.28

-95.13%

-97.88%

-100.00%

5.2
-120.00%

5
4.8

4.73

PRECISION, STD. DEV, REPEATABILITY, CV

4.6
9.00%

4.4

8.00%

7.74%

7.00%
6.00%

TEST
VOLUME
200
AVG
9.7
STD
0.2465

11
10.5

5.00%
10.49

4.00%

10

3.00%

9.5

2.00%

9.01

1.00%

8.5

0.00%

-1.00%

2.53%
1.83%
UCL, 0.40%

UCL, 0.15%
UCL, 0.25%

50

LCL, -0.40%

UNIVERSITY INSTRUMENTATION CENTER

220 Parsons Hall, 23 College Road, University of New Hampshire, Durham, NH 03824
TEL: (603) 862-2790, FAX: (603) 862-4104, URL: www.unh.edu/uic

100

LCL, -0.25%

200

LCL, -0.15%

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A
Cell Link
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B
LAST
Amato-Wierda
Anderson
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C
FIRST
Carmela
Franz
Kim
Alan
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Christopher
John
David
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Alfred
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Wallace
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Dave
Tamara
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Nancy
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D
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Kocher
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Spear
Steinberg
Stewart
Stubbs
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Tagliaferro
Taylor
Taylor
Thomas
Tillinghast
Tisa

C
Tom
Dean
Glen
James
Jo
Deb
Andy
Tom
Michael
Amy
Marian
Ted
Brent
Susan
Kay
Alison
Aaron
Virendra
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Jeff
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Bob
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Michelle
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Sam
Zach
Sara
Susan
Stacia
Jeannie
Barbara
Gail
Jeff
Elise
Susan
Tony
Robert
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William
Ed
Louis

D
2-2115
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F
Enviornmental Tech Bldg
Spaulding
Rudman Hall
Parsons Hall
James Hall
Jackson Lab
Rudman Hall
Rudman Hall
Rudman Hall
Parsons Hall
Rudman Hall
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Spaulding
Rudman Hall
Brook House
Morse Hall
Rudman Hall
Parsons Hall
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James Hall
Kingsbury
Parsons Hall
27 Mast Road Durham
Rudman Hall
Rudman Hall
Kendall hall
UNH Manchester
Spaulding
Rudman Hall
Parsons Hall
Morse hall
Parsons Hall
Rudman Hall
Rudman Hall
Parsons Hall
Kendall hall
Spaulding
Rudman Hall
Rudman Hall
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Rudman Hall
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Parsons Hall
Jackson Lab
Green Houses
Kendall hall
Byrand Hall, UMaine Orno
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PCAC
Spaulding
Parsons Hall
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Poultry
Kendall hall
Enviornmental Tech Bldg
Rudman Hall
Spaulding
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Parsons Hall

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A
126
127
128
129
130
131
132
133
134
135
136
137
138
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140
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142

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Tomellini
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Tsang
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VonDamm
Walker
Watson
Weisman
Whistler
Whitehouse
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Wong
Wrightsman
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Zercher

C
Sterling
Jane
David
Paul
Carl
Karen
Charles
Win
Gary
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Jon
Nancy
Sally
Ed
Linda
Dan
Charles

D
2-2519
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2-0142
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2-1027
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Complex Systems Research Cntr
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Microbiology
Dairy Complex
Animal Nutritional Sciences
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Chemistry
Earth Sciences
Natural Resources
Chemistry

F
Health Sevrvices
Kendall hall
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Rudman Hall
Parsons Hall
Dairy Barn
Rudman Hall Rm. 222
Ritzman Lab
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Parsons Hall
James Hall
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Parsons Hall
Rudman Hall

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MODEL
Pipetman P-2
Pipetman P-10
Pipetman P-20
Pipetman P-100
Pipetman P-200
Pipetman P-200-M8
Pipetman P-1000
Pipetman P-5000
Pipetman P-10ML
Rainin Pipet-Lite, Pipet-Plus, or EDP (2uL)
Rainin Pipet-Lite, Pipet-Plus, or EDP (10uL)
Rainin Pipet-Lite, Pipet-Plus, or EDP (20uL)
Rainin Pipet-Lite, Pipet-Plus, or EDP (100uL)
Rainin Pipet-Lite, Pipet-Plus, or EDP (200uL)
Rainin Pipet-Lite, Pipet-Plus, or EDP (1000uL)
Rainin Pipet-Lite, Pipet-Plus, or EDP (2000uL)
Rainin Pipet-Lite, Pipet-Plus, or EDP (5000uL)
Rainin Pipet-Lite, Pipet-Plus, or EDP (10mL)
DISTRIMAN 125 UL TIP
DISTRIMAN 1250 UL TIP
DISTRIMAN 12.5 mL TIP
MICROMAN M-10
MICROMAN M-25
MICROMAN M-50
MICROMAN M-100
MICROMAN M-250
MICROMAN M-1000
Rainin E2 or EP (10uL)
Rainin EDP, Black Only (10uL)
Rainin EDP, EDP2, or EDP-Plus (25uL)
Rainin EDP, EDP2, or EDP-Plus (100uL)
Rainin EDP, EDP2, or EDP-Plus (250uL)
Rainin EDP, EDP2, or EDP-Plus (1000uL)
Rainin EDP, EDP2, or EDP-Plus (2500uL)
Rainin EDP, EDP2, or EDP-Plus (10 mL)
Eppendorf Adjustable (0.1 - 2.5 uL Anthracite)
Eppendorf Adjustable (0.5 - 10 uL Gray)
Eppendorf Adjustable (2 - 20 uL Gray)
Eppendorf Adjustable (2 - 20 uL Yellow)
Eppendorf Adjustable (10 - 100 uL Yellow)
Eppendorf Adjustable (50 - 200 uL Yellow)
Eppendorf Adjustable (100 - 1000 uL Blue)
Eppendorf Adjustable (500-2,500 uL Red)
Eppendorf Research 2.5 uL
Eppendorf Research 10 uL
Eppendorf Research 20 uL
Eppendorf Research 100 uL
Eppendorf Research 200 uL
Eppendorf Research 1000 uL
Eppendorf Research 5000 uL
Corning Lambda (0.1 - 2.0 uL) Aqua
Corning Lambda (0.5 - 10 uL) Orange

C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
TEST VOL ACCURACY PRECISION TEST VOL ACCURACY PRECISION TEST VOL ACCURACY PRECISION Pipette Cell Link Accuracy Accuracy Accuracy Accuracy Accuracy Accuracy
# 1 (uL)
%(+/-)
%(+/-)
# 2 (uL)
%(+/-)
%(+/-)
# 3 (uL)
%(+/-)
%(+/-)
5
High Limit Low Limit High Limit Low Limit High Limit Low Limit
0.2
12
6
1
2.7
1.3
2
1.5
0.7
0.224
0.176
1.027
0.973
2.03
1.97
1
2.5
1.2
5
1.5
0.6
10
1
0.4
1.025
0.975
5.075
4.925
10.1
9.9
2
7.5
2
10
1.5
0.5
20
1
0.3
2.15
1.85
10.15
9.85
20.2
19.8
10
3.5
1
50
0.8
0.24
100
0.8
0.15
10.35
9.65
50.4
49.6
100.8
99.2
50
1
0.4
100
0.8
0.25
200
0.8
0.15
50.5
49.5
100.8
99.2
201.6
198.4
20
2.5
1.25
100
1
0.5
200
1
0.5
20.5
19.5
101
99
202
198
100
3
0.6
500
0.8
0.2
1000
0.8
0.15
103
97
504
496
1008
992
500
2.4
0.6
2500
0.6
0.2
5000
0.6
0.16
512
488
2515
2485
5030
4970
1000
5
0.6
5000
1
0.2
10000
0.8
0.16
1050
950
5050
4950
10080
9920
0.2
12
6
1
2.7
1.3
2
1.5
0.7
0.224
0.176
1.027
0.973
2.03
1.97
1
2.5
1.2
5
1.5
0.6
10
1
0.4
1.025
0.975
5.075
4.925
10.1
9.9
2
7.5
2
10
1.5
0.5
20
1
0.3
2.15
1.85
10.15
9.85
20.2
19.8
10
3.5
1
50
0.8
0.24
100
0.8
0.15
10.35
9.65
50.4
49.6
100.8
99.2
20
2.5
1
100
0.8
0.25
200
0.8
0.15
20.5
19.5
100.8
99.2
201.6
198.4
100
3
0.6
500
0.8
0.2
1000
0.8
0.13
103
97
504
496
1008
992
200
3
0.6
1000
0.8
0.2
2000
0.8
0.12
206
194
1008
992
2016
1984
500
2.4
0.6
2500
0.6
0.2
5000
0.6
0.16
512
488
2515
2485
5030
4970
1000
5
0.6
5000
1
0.2
10000
0.8
0.16
1050
950
5050
4950
10080
9920
2
5
4
5
2.5
1.5
10
2
1
2.1
1.9
5.125
4.875
10.2
9.8
20
4
1
50
2
0.8
100
1
0.6
20.8
19.2
51
49
101
99
200
3
0.5
500
1.5
0.3
1000
1
0.25
206
194
507.5
492.5
1010
990
1
6
2
5
2.5
0.6
10
1.5
0.5
1.06
0.94
5.125
4.875
10.15
9.85
3
5
2
10
1.7
0.8
25
1
0.4
3.15
2.85
10.17
9.83
25.25
24.75
20
1.7
0.6
50
1
0.3
N/A
N/A
N/A
20.34
19.66
50.5
49.5 #VALUE! #VALUE!
10
5
2
50
1.5
0.6
100
1
0.4
10.5
9.5
50.75
49.25
101
99
50
3
0.4
100
1.7
0.3
250
1
0.2
51.5
48.5
101.7
98.3
252.5
247.5
200
1.5
0.8
500
1
0.5
1000
0.8
0.4
203
197
505
495
1008
992
1
2.5
1.2
5
1.5
0.6
10
1
0.4
1.025
0.975
5.075
4.925
10.1
9.9
1
10
4
5
2
2
10
1
0.5
1.1
0.9
5.1
4.9
10.1
9.9
2.5
6
2
12.5
1.2
0.4
25
1
0.3
2.65
2.35
12.65
12.35
25.25
24.75
10
3
1
50
0.8
0.2
100
0.8
0.2
10.3
9.7
50.4
49.6
100.8
99.2
25
2
0.6
125
0.8
0.15
250
0.8
0.15
25.5
24.5
126
124
252
248
100
3
0.6
500
0.8
0.13
1000
0.8
0.13
103
97
504
496
1008
992
250
3.2
0.8
1250
0.8
0.16
2500
0.8
0.12
258
242
1260
1240
2520
2480
1000
5
0.6
5000
1
0.2
10000
0.8
0.16
1050
950
5050
4950
10080
9920
0.25
12
6
1.25
2.5
1.5
2.5
1.4
0.7
0.28
0.22 1.28125 1.21875
2.535
2.465
1
2.5
1.8
5
1.5
0.8
10
1
0.4
1.025
0.975
5.075
4.925
10.1
9.9
2
3
2
10
1
0.5
20
1
0.3
2.06
1.94
10.1
9.9
20.2
19.8
2
5
1.5
10
1.2
0.6
20
1
0.3
2.1
1.9
10.12
9.88
20.2
19.8
10
2.5
0.7
50
0.8
0.3
100
0.8
0.15
10.25
9.75
50.4
49.6
100.8
99.2
50
1
0.3
100
0.9
0.3
200
0.6
0.2
50.5
49.5
100.9
99.1
201.2
198.8
100
1.6
0.3
500
0.6
0.2
1000
0.6
0.2
101.6
98.4
503
497
1006
994
500
1.5
0.3
1000
0.8
0.2
2500
0.6
0.2
507.5
492.5
1008
992
2515
2485
0.2
12
6
1
2.5
1.5
2.5
1.4
0.7
0.224
0.176
1.025
0.975
2.535
2.465
1
2.5
1.8
5
1.5
0.8
10
1
0.4
1.025
0.975
5.075
4.925
10.1
9.9
2
5
1.5
10
1.2
0.6
20
1
0.3
2.1
1.9
10.12
9.88
20.2
19.8
10
3
1
50
1
0.3
100
0.8
0.2
10.3
9.7
50.5
49.5
100.8
99.2
20
2.5
0.7
100
1
0.3
200
0.6
0.2
20.5
19.5
101
99
201.2
198.8
100
3
0.6
500
1
0.2
1000
0.6
0.2
103
97
505
495
1006
994
500
2.4
0.6
2500
1.2
0.25
5000
0.6
0.15
512
488
2530
2470
5030
4970
0.2
12
6
1
2.5
1.5
2.5
1.4
0.7
0.224
0.176
1.025
0.975
2.535
2.465
0.5
5
2.8
1
2.5
1.8
10
1
0.4
0.525
0.475
1.025
0.975
10.1
9.9

Conversion factor based on Equation (1), Page F-6, from table entitled, "Volume Properties of Water at 1 atm".
Handbook of Chemistry and Physics, 64th Edition (1983-1984), Published by CRC Press.

p,kg/m ^ -3 = (999.83952 + 16.945176 t - 7.9870401 X 10 ^ -3 t ^ 2 - 46.170461 X 10 ^ -6 t ^ 3 + 105.5

Note that the result from the equation above is divided by 1000 yielding units of g/ml - the desired result.
TEMPERATURE
DEG C

10

CONVERSION
FACTOR
g / mL

Term 1

Term 2

Term 3

Term 4

0.999700 999.8395200 169.4517600 -0.7987040 -0.0461705

f Water at 1 atm".

0461 X 10 ^ -6 t ^ 3 + 105.56302 X 10 ^ -9 t ^4 - 280.54253 X 10 ^ -12 t ^ 5) / (1 + 16.8734850 X 10 ^ -3 t)

ired result.

Term 5

Term 6

0.0010556 -0.0000281

BALANCE
CELL LINK

MODEL

1 Mettler Toledo MX5

2 Mettler Toledo AB54-S

MAX
CAPACITY
grams
5.1
51
Maximum amount
balance can weigh.

MAX
READABILITY
uG
100
1
Maximum readable
weight displayed on
balance.

ECCENTRICITY

REPEATABILITY

HYSTERESIS

LINEARITY

SPAN

uG
3
200

uG
0.8
100

uG
4
200

uG
4
200

uG
30
200

The acceptable error

when the weight is
placed to the far edge
of the load pan.

Also called precision.

Tells you that if you
weigh ten times result
will fall within 2 *
repeatability

CELL
LINK

LINK
1 Rob Cinq-Mars
2 John Wilderman
3
4
5
6
7
8
9
10

TEST RESULTS

Inspection
Leakage
Cleaning
Seal Replacement
O-Ring Replacement

TEST

Sample #
1
2
3
4
5
6
7
8
9
10
AVG
STD
MIN
MAX

VOL
50
0.927721
1.020693
1.06668
1.225632
1.076677
1.104668
1.077676
0.958712
1.055683
1.095671
1.060981
0.082089
0.927721
1.225632

TRUE
TRUE
TRUE
TRUE
TRUE

+/- 3 STD DEV

-3 STD
0.81
0.81
0.81
0.81
0.81
0.81
0.81
0.81
0.81
0.81
0.81

+3 STD
1.31
1.31
1.31
1.31
1.31
1.31
1.31
1.31
1.31
1.31
1.31

1.060981
1.060981
1.060981
1.060981
1.060981
1.060981
1.060981
1.060981
1.060981
1.060981

TEST
VOL
100
5.002497
5.002497
4.902527
5.175445
5.067477
4.870537
4.939516
5.09347
4.987501
5.047483
5.008895
0.091665
4.870537
5.175445
5.008895
5.008895
5.008895
5.008895
5.008895
5.008895
5.008895
5.008895
5.008895
5.008895

ACCURACY or Mean Error is the mean

volume - volume setting. As a percentage it
is ((mean volume - volume setting) / Volume
Setting)*100

Volume
Setting
50
100
200

% ACCURACY
Mean
LCL
Error
-97.88% -1.00%
-94.99% -0.80%
-95.13% -0.80%

UCL
1.00%
0.80%
0.80%

+/- 3 STD DEV

-3 STD
4.73
4.73
4.73
4.73
4.73
4.73
4.73
4.73
4.73
4.73
4.73

+3 STD
5.28
5.28
5.28
5.28
5.28
5.28
5.28
5.28
5.28
5.28
5.28

TEST
VOL
200
9.947011
9.872034
9.845042
9.800056
9.836045
9.672094
9.658098
9.161247
9.61911
10.05998
9.747071
0.246515
9.161247
10.05998

+/- 3 STD DEV

-3 STD
9.01
9.01
9.01
9.01
9.01
9.01
9.01
9.01
9.01
9.01
9.01

+3 STD
10.49
10.49
10.49
10.49
10.49
10.49
10.49
10.49
10.49
10.49
10.49

9.747071
9.747071
9.747071
9.747071
9.747071
9.747071
9.747071
9.747071
9.747071
9.747071
PRECISION also known as Standard
Deviation, reproducibility and repeatability.
Calculated using @std function.

% PRECISION
Volume
STD as %
LCL
Setting
50
7.74% -0.40%
100
1.83% -0.25%
200
2.53% -0.15%

UCL
0.40%
0.25%
0.15%

TEST
VOLUME
S
50
100
200

ACCURACY

PRECISION

PERCENT + / -

PERCENT + / -

1
0.8
0.8

0.4
0.25
0.15

RAW DATA
0.928
1.949
3.016
4.242
5.319
6.424
7.502
8.461
9.517
10.613

5.004
10.008
14.912
20.089
25.158
30.03
34.971
40.066
45.055
50.104

9.95
19.825
29.673
39.476
49.315
58.99
68.651
77.815
87.437
97.5