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  • Blunt End: Molecular Biology DNA Fragment DNA Molecule

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    A blunt end in molecular biology refers to a double-stranded DNA fragment where both strands are of equal length with no unpaired bases or overhangs. In contrast, a sticky end results from a staggered cut that leaves a stretch of unpaired nucleotides at the terminus, making the strands unequal in length and allowing the ends to bind through complementary base pairing in the overhanging region.

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    Blunt end

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    A blunt end in molecular biology refers to a double-stranded DNA fragment where both strands are of equal length with no unpaired bases or overhangs. In contrast, a sticky end results from a staggered cut that leaves a stretch of unpaired nucleotides at the terminus, making the strands unequal in length and allowing the ends to bind through complementary base pairing in the overhanging region.

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    8 views1 page

    Blunt End: Molecular Biology DNA Fragment DNA Molecule

    Uploaded by

    Careless Whisperr
    A blunt end in molecular biology refers to a double-stranded DNA fragment where both strands are of equal length with no unpaired bases or overhangs. In contrast, a sticky end results from a staggered cut that leaves a stretch of unpaired nucleotides at the terminus, making the strands unequal in length and allowing the ends to bind through complementary base pairing in the overhanging region.

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    Blunt end

    (molecular biology) The end of a DNA fragment resulting from the breaking of DNA molecule in which there are no unpaired bases, hence, both strands are of the same length. In molecular biology, a blunt end may result from the breaking of double-stranded DNA; however, there are no overhangs or unpaired bases. An example of a blunt end: 5'-CTGATCTGACTGATGCGTATGCTAGT-3' 3'-GACTAGACTGACTACGCATACGATCA-5'

    Sticky end
    (molecular biology) A fragment of DNA (often produced by a staggered cut on the DNA using restriction enzymes) in which the terminal portion has a stretch of unpaired nucleotides, and the strands are not of the same length. The ends of the DNA are sticky because it can easily match up with a complementary overhang. For example, these two sticky ends can be joined together since they can form complementary base pairs in the overhang region: 5'-ATCTGACT ______+ GATGCGTATGCT-3' 3'-TAGACTGACTACG________CATACGA-5'

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