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  • May Grunwald-Giemsa Staining

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    Salma Khalid
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    This document provides instructions for May-Grunwald/Giemsa staining of cytospin slides. The steps include: immersing dried slides in May-Grunwald dye for 4 minutes, directly transferring to 4% Giemsa dye for another 4 minutes, briefly rinsing in water, wiping excess dye off the slides, and allowing them to air dry. An alternative fixation method using -20°C acetone for 10 minutes before drying is also described. Finally, cytoseal is applied and a coverslip is added and pressed firmly in place to complete the staining process.

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    This document provides instructions for May-Grunwald/Giemsa staining of cytospin slides. The steps include: immersing dried slides in May-Grunwald dye for 4 minutes, directly transferring to 4% Giemsa dye for another 4 minutes, briefly rinsing in water, wiping excess dye off the slides, and allowing them to air dry. An alternative fixation method using -20°C acetone for 10 minutes before drying is also described. Finally, cytoseal is applied and a coverslip is added and pressed firmly in place to complete the staining process.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    268 views1 page

    May Grunwald-Giemsa Staining

    Uploaded by

    Salma Khalid
    This document provides instructions for May-Grunwald/Giemsa staining of cytospin slides. The steps include: immersing dried slides in May-Grunwald dye for 4 minutes, directly transferring to 4% Giemsa dye for another 4 minutes, briefly rinsing in water, wiping excess dye off the slides, and allowing them to air dry. An alternative fixation method using -20°C acetone for 10 minutes before drying is also described. Finally, cytoseal is applied and a coverslip is added and pressed firmly in place to complete the staining process.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    May Grunwald/Giemsa Staining for Cytospin Slides

    Reagents: May-Grunwald, 4% Giemsa, water, (alternative: -20o acetone), cytoseal. Equipment: containers for dye, cover slips, kimwipes.
    As a note of caution; do not use markers on slides which will be stained. Use

    only a pencil. Markers will bleed into the stain and ruin the dyes.
    Remove slides from holders and allow to dry Immerse slides in 100% May-Grunwald for 4 minutes Transfer slides directly to 4% (diluted in tap water) Giemsa for 4 minutes. Rinse slides very briefly in water Wipe excess dye off back of slide and air dry Alternative method: To fix slides without staining place in -20 acetone for 10
    o

    minutes, remove from acetone and allow to dry


    Place small drop of cytoseal on slide near cells Place cover slip on slide and press firmly with back of a pipet tip, air dry

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