Professional Documents
Culture Documents
PERFECT
SCORE
BIOLOGY
2009
MODUL III
PAPER3
QUESTION1
Students Copy
DISEDIAKAN OLEH:
1.
2.
3.
4.
5.
6.
7.
PERFECTSCOREBIOLOGY 2009
Answer all questions.
1. A group of students carried out an experiment to study the effect of temperature to rate of plant
transpiration.
Diagram 1(a) shows an experiment set-up of the apparatus used in this experiment. The apparatus
are prepared and kept in laboratory with difference temperature. The whole experiment in Diagram 1
was repeated using different temperature.
Diagram 1 (b) shows the position of the meniscus at the initial of the experiment. Two points, P and
Q are mark with 10 cm distance at the tube of potometer.
Table 1.1 shows the reading of temperature and location of meniscus in the tube of potometers after 5
minutes.
PERFECTSCOREBIOLOGY 2009
Thermometers
after 5 minutes
Table 1.1
Table 1.1
(a) (i)
Based on table 1.1 state two observations on the relationship between the
temperature and meniscus.
PERFECTSCOREBIOLOGY 2009
1. ...........................................................................................................................
.........................................................................................................................................................
2. .......................................................................................................................................................
........................................................................................................................................................
[3 marks]
(ii) State the inference which corresponds to the observations in 1 (a)(i)
1. .................................................................................................................................................
......................................................................................................................................................
2. .................................................................................................................................................
........................................................................................................................................................
[3 marks]
(b) Complete the Table 1.1 by recording the temperature and the final position of meniscus.
[3 marks]
(c) (i) Complete the Table 1.2 base on the experiment that was carried out.
Variables
Manipulated variable
.......................................................
.......................................................
.......................................................
.......................................................
Responding variable
.......................................................
.......................................................
.......................................................
.......................................................
Controls variable
.......................................................
.......................................................
.......................................................
.......................................................
[3 marks]
Table 1.2
(ii) The following list is part of the apparatus and material used in this experiment.
Thermometer, stop watch, ruler, capillary tube, plant leaves, water
PERFECTSCOREBIOLOGY 2009
Complete Table 1.3 by matching each variable with the apparatus and material used in this
experiment.
Variable
Apparatus
material
Manipulated
Responding
Controlled
[3 marks]
Table 1.3
(d) State the hypothesis for this experiment.
.....................................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
[3 marks]
(e) (i) Base on the Table 1.1, construct the table and record the results of this experiment which includes
following aspect:
Temperature (oC)
The distance of meniscus movement (cm)
Rate of transpiration (cm/minute)
[3 marks]
(ii) On the graph paper, draw the graph of rate of transpiration against the
temperature.
[3 marks]
PERFECTSCOREBIOLOGY 2009
(iii) Explain the relationship between the rate of transpiration and the temperature
based on the graph.
.......................................................................................................................................................................
PERFECTSCOREBIOLOGY 2009
.......................................................................................................................................................................
.......................................................................................................................................................................
[3 marks]
(f) Based on the experiment, what can you deduce about the transpiration?
........................................................................................................................................................................
........................................................................................................................................................................
........................................................................................................................................................................
[3 marks]
(g) In another experiment, the apparatus is located under the sun shine.
Predict the observation and explain the results of the experiment.
.....................................................................................................................................................................
.....................................................................................................................................................................
......................................................................................................................................................................
[3 marks]
Question 2:
An experiment was done to study the effect of pH on the activity of amylase. Three test tubes labelled P,
Q and R are filled with 10ml of amylase and later filled with 40ml distilled water, 40ml hydrochloric acid
and 40ml sodium bicarbonate respectively.
PERFECTSCOREBIOLOGY 2009
The 50ml of starch suspension was put into each test tube and the contents were stirred.
Every two minutes a drop of the mixture from each test tube was dropped into a drop of iodine solution on
a white tile.
Changes in colour of iodine are observed. The figure shows the set of apparatus of the experiment.
(a)
(i)
PERFECTSCOREBIOLOGY 2009
2. .
.
[3 marks]
(ii)
.
..
2. .
.
[3 marks]
b)
Record the time taken when the iodine solution stopped changing colour in the boxes provided.
[3 marks]
c)
Particulars to be implemented
How to alter the manipulated variable?
..
..
Responding variable
..
Constant variable
.
..
(3 marks)
e)
PERFECTSCOREBIOLOGY 2009
f)
[3 marks
g)
What is the optimum pH for the activity of amylase? Give reasons for your answer.
...
...
h)
[3 marks]
Explain the relationship between the time taken for change in colour of iodine from yellowish brown
to blue black and different pH medium.
.
.....
....
[3 marks]
i)
Predict the time taken for change in colour of iodine from yellowish brown to blue black if 40ml of
amylase is used in boiling tube P. Explain your answer.
.
....
.
[3 marks]
j)
10
PERFECTSCOREBIOLOGY 2009
..
[3 marks]
k)
From your biological knowledge, match the substrate with the enzymes below:
Protein, amylase, trypsin, maltose, starch, maltase, pepsin.
. .
..
.
..
[3 marks]
Question 3:
A group of students carried out an experiment to study the inheritance of the colour of flowers in a
plant. The colour of flower is controlled by single pair of alleles, red and white.
The students used red and white button to represents the alleles for red and white flowers.
Two black plastic bag, A and B, each containing a mixture of 50 red buttons and 50 white buttons
were used to illustrate the cross.
11
PERFECTSCOREBIOLOGY 2009
The students carried out the experiment as follows:
Step 1: One button was drawn at random from each bag.
Step 2: The colour of the button was noted and recorded in a table.
Step 3: The buttons were returned to their respective bags.
Step 4: The bags A and B were shaken to mix up the button randomly.
Step 5: Step 1 to 4 were repeated until a total of 20 times.
12
PERFECTSCOREBIOLOGY 2009
(a) (i) State two observations from the experiment based on the results shown in Table 1.1
[3 marks]
1. ..
..
2. ..
.
..
..
2.
..
.
(b) Complete table 1.2 based on the experiment that was carried out.
Variable
Manipulated variable:
..
.
Responding variable:
..
.
Controlled variable:
..
.
[3 marks]
(c) State the hypothesis for the experiment.
[3 marks]
...
(d) (i)If the allele for red flowers ( R) is dominant to the allele for white flower (r) , write down
genotypes of all the draws in the table 1.1 using the letter R and r.
[3 marks]
the
13
PERFECTSCOREBIOLOGY 2009
(ii) Determine and write down the phenotype of each draw in table 1.1 From the results in table 1.1,
calculate the ratio of the phenotypes obtained from the experiment.
[3 marks]
...
.
[3 marks]
(f) State the effect of the number of draws on the result obtained.
[3 marks]
...
.
(g) Based on the experiment on the inheritance of the colours of flowers that the students had done,
define Mendels First Law of inheritance.
[3 marks]
...
.
(h) The experiment is repeated by increasing the numbers of red and white buttons from 50 to 100 in
each bag. Predict the ratio of the phenotypes that you would obtain.
[3 marks]
...
..
(i)
Classify the list of materials shown below according to their function in the experiment.
[3 marks]
Material
Function
14
PERFECTSCOREBIOLOGY 2009
Question 5
An experiment is carried out to investigate the content of vitamin C in certain fruit juices. The method of
experiment is conducted as follows:
1. Preparation of fruit juices shown in the diagram 5.0 below.
The squashed is
filtered to obtain the
juice.
Fruit is squashed
Diagram 5.0
Fruit
juices
DIAGRAM 3.0
2.
3.
4.
Volume of ascorbic
acid 0.1% used:
5 ml syringe
Ascorbic
acid 0.1%
15
1 ml
DCPIP
0.1% (dark blue)
Colourless
PERFECTSCOREBIOLOGY 2009
Before Experiment
After Experiment
Lime juice
16
PERFECTSCOREBIOLOGY 2009
Pineapple juice
(a)
(i)
2.
(ii)
Observation 2:
..................................................................................................................................
(b)
Lime juice
Pineapple juice
Orange juice
17
PERFECTSCOREBIOLOGY 2009
(c)(i)
Manipulated variable
.
Responding variable
Controlled variable
[3 marks]
(d) State the hypothesis for this experiment.
[3 marks]
..................................................................................................................................
18
PERFECTSCOREBIOLOGY 2009
(f) Explain the relationship between the volume of fruit juice required to decolourise DCPIP
solution and the concentration of vitamin.
[3 marks]
..................................................................................................................................
..
(g) The following lists are some of the apparatus and material used in this experiment.
Syringe 5.0 ml, watermelon juice, specimen tube, DCPIP solution, 0.1
% ascorbic acid solution, scalpel.
(h) (i) If lime juice is exposed to the air for several hours, predict the volume of lime juice
required to decolourise DCPIP solution.
[3 marks]
............................................................................................................................................
19
PERFECTSCOREBIOLOGY 2009
..
.
(ii) explain the relationship between volume of lime juice required to decolourise DCPIP
solution and the time based on (h)(i).
[3 marks]
............................................................................................................................................
..
..
(i) Based on this experiment, define vitamin C operationally.
[3 marks]
..................................................................................................................................
Question 6 :
A group of students carried out an experiment to determine the concentration of external solution which
is isotonic to the cell sap of a plant.
Figure1.1 shows the set up of apparatus for this experiment. By using a cork borer, a cylindrical potato
strip was obtained. The potato strip was cut into 5 cm long.
After 30 minutes , the potato strip is measured again and the final length of potato strip is recorded. The
measurement of potato strip is shown in figure 1.2.
The experiment in figure1.1 was repeated by using different concentration of sucrose solutions.
0.0% Sucrose
solution
Potato strip
(initial length
5cm)
Figure 1.1
Potato strip
Ruler
20
PERFECTSCOREBIOLOGY 2009
21
PERFECTSCOREBIOLOGY 2009
Table 1.1 shows the results of the experiment.
Concentr
ation
of
sucrose
solution
(%)
Final length of
potato strip
(cm)
0.5
5%
10%
15%
TABLE 1.1
(a)
Measure and record the length of potato strips and complete the table 1.1.
22
PERFECTSCOREBIOLOGY 2009
(b) (i) Based on Table 1.1 state two observations that can be made from the experiment concerning the
length of cylindrical potato strips.
............................................................................................................................................
..
[ 3 marks]
(ii)
............................................................................................................................................
..
[ 3 marks]
c)
Complete Table 1.2 based on this experiment. State the type of variable of the experiment and
method to handle the variable.
Variable
Manipulated variable
Responding variable
Controlled variable
[ 3 marks]
Table 1.2
23
PERFECTSCOREBIOLOGY 2009
(d) State the hypothesis for this experiment.
............................................................................................................................................
..
[ 3 marks]
(e) (i) Based on table 1.1 construct a table and record the results of the experiment
which includes the following aspects:
o
o
o
o
Aspect
0.5
10
15
Initial length/cm
Final length/cm
Change in length/cm
Percentage change in length/%
24
PERFECTSCOREBIOLOGY 2009
(ii) On the graph paper provided, draw the graph of the percentage change in length of
the potato strips against the concentration of the sucrose solutions .
[ 3 marks]
(iii) Based on the graph in (d )(ii), state the concentration of the sucrose solution which is
25
PERFECTSCOREBIOLOGY 2009
isotonic to the concentration of the cell sap of the potato strip. Explain your answer.
............................................................................................................................................
..
[ 3 marks]
(f)
............................................................................................................................................
..
[ 3 marks]
(g)
The potato strip that was immersed in 15% sucrose solution is wipe dry and immerse into the
0.2% sucrose solution.
Predict the changes that occur to the strips?
............................................................................................................................................
..
[ 3 marks]
(h)
[ 3 marks]
Question 7
26
PERFECTSCOREBIOLOGY 2009
An experiment is carried out to investigate the effect of environmental factor on the activity of
yeast.
Yeast reacts with sucrose solution to produce alcohol and carbon dioxide gas.
The apparatus as shown in Diagram 1.1 is set up.
1cm3 of 1% yeast suspension is transferred using a pipette into a boiling tube P containing 5
cm3 of 1% sucrose solution. The initial level of the coloured liquid in the U tube is set at 0.0 mm.
After exactly 10 minutes, the level of the coloured liquid is observed as shown in Diagram 1.
Diagram 1.1
The experiment is repeated by using boiling tubes Q, R and S fill with same volume of sucrose
solution. The concentrations of solution used are 2%, 3% and 4% .
27
PERFECTSCOREBIOLOGY 2009
The changes of the coloured liquid levels in the U-tube are shown in Diagram 1.2
25.0 mm
35.0mm
45.0mm
Diagram 1.2
(a) (i)
55.0mm
(3 marks)
Based on the results in Diagram 1.1 and 1.2 , state two observations that can be
made from the experiment.
............................................................................................................................................
..
[ 3 marks]
(a) (ii)
[ 3 marks]
(c)
Record the levels of the coloured liquid in the U-tubes in the spaces provided in Diagram
1.2.
[3 marks]
28
PERFECTSCOREBIOLOGY 2009
(d) (i) Complete Table 1.1 based on this experiment
Variable
Manipulated variable
Responding variable
Controlled variable
Table 1.1
[ 3 marks]
(ii). The following list of apparatus and materials are used in this experiment.
Pipette, 1% yeast solution, scale, coloured liquid, 2% to 5% sucrose solution.
Complete the table 1.2 below by matching each variables with the materials used in this
experiment
Variables
Apparatus
Materials
Manipulated
Responding
Constant
[ 3 marks]
Table 1.2
(e)
[ 3 marks]
(f) (i)
The volume of the carbon dioxide produced by the yeast in the boiling tube can be
calculated using the following formula:
The volume of the carbon dioxide produced (ml) = The cross-section of the glass tube
(mm2) X the distance travelled by the
coloured liquid (mm)
29
PERFECTSCOREBIOLOGY 2009
The volume of carbon dioxide produced (mm3)
The rate of carbon dioxide produced = --------------------------------------------------------------------Time taken for the coloured liquid to travel (s)
Based on Figure 2, record the results of this experiment obtained in (c) in a table, given that
the cross section of the glass tube is 3 mm2. Your table should include the following aspects:
The concentration of the sucrose solution
The distance travelled by the coloured liquid
The rate of carbon dioxide produced
(3 marks)
30
PERFECTSCOREBIOLOGY 2009
(ii)
From the table in (f) (i) above, draw a graph of the concentration of the sucrose solution
against the rate of carbon dioxide produced.
[ 3 marks]
31
PERFECTSCOREBIOLOGY 2009
(g) From the table you have drawn in (f) above, explain the relationship between the
concentration of sucrose solution, the height of the coloured liquid and the rate of carbon
dioxide produced by the yeast.
............................................................................................................................................
..
[ 3 marks]
(h)
............................................................................................................................................
..
[ 3 marks]
(i)
If the sucrose solution concentration is increased to 6%, predict the rate of carbon
dioxide produced by the yeast in 10 minutes.
............................................................................................................................................
..
[ 3 marks]
Question 8
32
PERFECTSCOREBIOLOGY 2009
An experiment is carried out to investigate the pollution level of a river in W district.
orchard
Housing
area
Food
factory
Figure 1.1
Three samples of water are collected from the three location respectively, and named water
sample P, water sample Q and water sample R. The samples are brought to the school lab and
tests are conducted.
First, 100ml of the water sample P is measured using a measuring cylinder and poured into a
clean reagent bottle. 1ml of 0.1% methylene blue solution is added into the water sample using
a syringe. The mixture is stirred carefully with the needle of the syringe. The reagent bottle is
closed quickly and kept in a dark cupboard (Figure 1.2). The stop watch is started (Table 1.1)
and the colour of the solution is observed from time to time to determine the time taken for the
0.1% methylene blue solution to decolourise.
Figure 1.2
The above steps are repeated with water sample Q and water sample R. The time taken for the
solution to decolourise is recorded in table 1.1.
33
PERFECTSCOREBIOLOGY 2009
Water
sample
location
Treatment
Time taken
(min)
Table 1.1
(a) (i)
Based on the results in Table 1.1, state two observations for the experiment.
............................................................................................................................................
..
34
PERFECTSCOREBIOLOGY 2009
(3 marks)
(ii)
(3 marks)
(b)
Record the time taken for the decolourisation of 0.1% methylene blue solution in the
spaces provided in Table 1.1.
(3 marks)
Manipulated variable
Responding variable
Controlled variable
Table 2.1
(3 marks)
35
PERFECTSCOREBIOLOGY 2009
(d)
The following list of apparatus and materials are used in this experiment.
0.1% methylene blue solution, water samples, stop watch, syringe, reagent bottle,
measuring cylinder,
Complete the table 1.4 below by matching each variables with the materials used in this
experiment
Variables
Apparatus
Materials
Manipulated
Responding
Constant
(3 marks)
(3 marks)
f.
Based on Table 1.1, record the results of this experiment obtained in (c) in a table. Your
table should include the following aspects:
-
(g)
From the table you have drawn in (f) above, explain the relationship between the
location from orchard area and time taken for the decolourisation of 0.1% methylene
blue solution and the B.O.D. level of the water sample.
............................................................................................................................................
..
(3 marks)
36
PERFECTSCOREBIOLOGY 2009
g. From the experiment, what can be deduced about B.O.D. level of water samples ?
............................................................................................................................................
..
(3 marks)
h. If the number of houses in housing area increase predict the time taken and the level of
B.O.D. for water in location Q.
............................................................................................................................................
..
(3 marks)
END
37