Scribd Logo
Upload

Welcome to Scribd!

  • Summary Conclusion Qa QC

    Uploaded by

    Zed Zium
    15 views1 page
    This experiment aimed to determine the total viable aerobic count and presence of E. coli in Labisa pumila. Samples were weighed and diluted through tenfold dilution. Total viable cells were tested using pour plate and spread plate methods to show colonies on agar plates which were then counted. E. coli testing involved incubating samples in nutrient agar and transferring to MacConkey agar to incubate, then subculturing on MacConkey agar plates. The growth of red, non-mucoid colonies indicated the possible presence of E. coli. In conclusion, the objectives were successfully achieved though some tests could be improved with further identification of colony types and species.

    Original Description:

    Original Title

    Summary Conclusion Qa Qc

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This experiment aimed to determine the total viable aerobic count and presence of E. coli in Labisa pumila. Samples were weighed and diluted through tenfold dilution. Total viable cells were tested using pour plate and spread plate methods to show colonies on agar plates which were then counted. E. coli testing involved incubating samples in nutrient agar and transferring to MacConkey agar to incubate, then subculturing on MacConkey agar plates. The growth of red, non-mucoid colonies indicated the possible presence of E. coli. In conclusion, the objectives were successfully achieved though some tests could be improved with further identification of colony types and species.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    15 views1 page

    Summary Conclusion Qa QC

    Uploaded by

    Zed Zium
    This experiment aimed to determine the total viable aerobic count and presence of E. coli in Labisa pumila. Samples were weighed and diluted through tenfold dilution. Total viable cells were tested using pour plate and spread plate methods to show colonies on agar plates which were then counted. E. coli testing involved incubating samples in nutrient agar and transferring to MacConkey agar to incubate, then subculturing on MacConkey agar plates. The growth of red, non-mucoid colonies indicated the possible presence of E. coli. In conclusion, the objectives were successfully achieved though some tests could be improved with further identification of colony types and species.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    Summary There are 2 main objective of this experiment where the first one is to determine total viable aerobic

    count in Labisa pumila and second is to determine the presence of E. coli in Labisa pumila. In handling this experiment there are two main part of this experiment, where the first part is preparation of sample and second part is testing. The experiment is start by weight in the sample and tenfold dilution is prepared. On second part there are two main sub test which is total viable cell and test for specified microorganism. For total viable cell there are two methods which is pour plate method and spread plate method. Both of this method will produce or show a lot colony if microorganism on its surface of the agar and the average number of colony is count. On the test for specified microorganism, the sample first incubates in nutrient agar in conical flask in 35C for 2 day, and then it is transfer to MacConkey agar and incubates in 43C for 2 day. Then it is subculture on the petri dish containing MacConkey agar and incubate in 35C for 3 days. The result is the growth of red, non- mucoid colonies of gramnegative rods indicates the possible presence of E. coli.

    Conclusion Based on the experiment, it is prove that the objective of this experiment which is to determine total viable aerobic count in Labisa pumila and to determine the presence of E. coli in Labisa pumila is successful. The result of this experiment is shown a positive result which indicate that the sample is producing a standard result as in theory. However some of the testing need to further test, this is due to the most of the test that has been handle is only on the observation. If there is further test, each of the colony produce will be determine its type and its species. When it is determine, more specific function of microorganism can be known. Some of the procedure also can change in order to get more difference or in other word is one procedure to show a positive and another is show the negative result in order to get or differentiate the reaction happen.

    You might also like