DISCUSSION

Streaking is a technique used in microbiology to isolate a pure strain from a single species of
microorganism, often bacteria. A microbiological culture can be grown so that the organism
can be identified, studied or tested. A sterile cotton swab or inoculation loop was sterilized
and dipped in a broth or specimen containing bacteria. Then loop was then spread across one
quadrant of an agar plate containing a growth medium which has been sterilized in an
autoclave. This introduces a solution of the bacteria to substrates which provide them
nutrients. Choice of which growth medium to use depends on which microorganism was
being cultured and which was being selected for. Growth media was usually based on the
agar, gelatinous substance derived from seaweed. The loop was re-sterilized and dragged
across the inoculated quadrant of the streak plate. (Elbing Karen & Brent, Roger. (2002)).
This was done to collect some bacteria on the loop. The loop was spread around another
fourth of the plate much like the previous step. The loop was sterilized and the procedure was
repeated. Each time the loop gathers fewer and fewer bacteria until it gathers just one single
bacterial cell that can grow into a colony .The streak plate was then incubated, for 2 hours to
allow bacteria to reproduce. At the end of incubation there should be enough bacteria to form
visible colonies in the area touched by the inoculation loop.

There a 4 type of techniques used in this experiment. The first one was the transfer of
microbial culture from nutrient broth to agar plate by streaking. This was most common
method to observe colony morphology and to work with individual colonies for diagnostic
methods. The pattern of growth of bacteria shows in four different directions.

Figure 1 : Microbial culture from nutrient broth to agar plate by streaking

Second type technique had been used was transfer of microbial culture from nutrient broth to
agar slant by streaking method. Slant was a tube of solid medium at an angle. Tube was easy
to store and transport. The pattern of growth pf bacteria showed the zigzag pattern and can
see colony morphology but in small surface area.

Figure 2 : Microbial culture from nutrient broth to agar slant by streaking method

Third techniques used in this experiment were transfer of microbial culture from agar plate to
broth bottle. Broth was in a liquid medium. These techniques which in tube was easy to store
and transport but cannot saw colony morphology. After conducted the experiment, it showed
Pseudomonas sp. grown at the bottom.

Figure 3 : Microbial culture from agar plate to broth bottle

Last techniques had been used in this experiment was transfer of microbial culture from agar
plate to agar deep by stabbing technique. Agar deep was in tube of solid or semi-solid
medium. It was good for organisms that prefer reduced oxygen and to evaluate motility. The
result showed, the microbial of Pseudomonas sp. was formed a layer at the top in order it
needed for oxygen.

Figure 4 : Microbial culture from agar plate to agar deep by stabbing technique
REFERENCE

Elbing, Karen & Brent, Roger, (2002), Growth on Solid Media, Current protocols in
molecular biology Chapter 1, Unit 1.3.