Lab # 3

Title:
Determination of HCO3-2 in given water Sample. APPRATUS: Water sample, Methyl Orange, 0.01NH2 SO4, ConicalFlask, Burette, Stirror. Principle: Bicarbonates can determined by titrating against standard 0.01NH2SO4, using methyl Orange as an indicator. Procedure: 1. Add 2-3 drops of methyl orange in the same conical flask (after the end point for CO3) 2. Titrate it against N/10 H2SO4 to pinkish yellow end point. 3. Save the flask for Cl-1 determination. Calculation: Volume of irrigation water taken = 50ml Volume of N/10 H2SO4used = R2 Actual Volume of acid used = R2 R1 50ml of irrigation water contains HCO3= [(R2-R1) N/10] me 1000 ml water contains HCO3 = (R2-R1) (N/10) (1000/50) =2(R2-R1) meL-1 Precautions: 1. Use the same conical flask after the end point of CO3 determination, of CO3 is present. 2. Remove air bubbles present in the tip of the burette. 3. Shake the flask vigorously during titration. 4. R2 is the total amount of acid used to neutralize the bicarbonates present in the sample after the titration for HCO3 which means that the sample contains bicarbonates. Therefore the actual amount of acid used for neutralizing the originally present HCO3 would be R2- R1.