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    ELISA, or Enzyme-Linked Immunosorbent Assay, is a biochemical technique used to detect antibodies or antigens in a sample. There are different types of ELISA including direct, indirect, sandwich, and competitive ELISA. An example of indirect ELISA is detecting IgG, IgA, and IgM antibodies to Mycobacterium species in human serum. The ELISA procedure involves coating a microtiter plate with an antigen, adding the sample and enzyme-conjugated antibody, and detecting the bound enzyme with a substrate to produce a color change. Results are quantified using a standard curve to determine sample concentrations. ELISA offers advantages such as high sensitivity, quantifiability, and being well-suited for individual and mass screening.

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    ELISA, or Enzyme-Linked Immunosorbent Assay, is a biochemical technique used to detect antibodies or antigens in a sample. There are different types of ELISA including direct, indirect, sandwich, and competitive ELISA. An example of indirect ELISA is detecting IgG, IgA, and IgM antibodies to Mycobacterium species in human serum. The ELISA procedure involves coating a microtiter plate with an antigen, adding the sample and enzyme-conjugated antibody, and detecting the bound enzyme with a substrate to produce a color change. Results are quantified using a standard curve to determine sample concentrations. ELISA offers advantages such as high sensitivity, quantifiability, and being well-suited for individual and mass screening.

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    4 views11 pages

    Presentation 1

    Uploaded by

    Sreedivya Saladi
    ELISA, or Enzyme-Linked Immunosorbent Assay, is a biochemical technique used to detect antibodies or antigens in a sample. There are different types of ELISA including direct, indirect, sandwich, and competitive ELISA. An example of indirect ELISA is detecting IgG, IgA, and IgM antibodies to Mycobacterium species in human serum. The ELISA procedure involves coating a microtiter plate with an antigen, adding the sample and enzyme-conjugated antibody, and detecting the bound enzyme with a substrate to produce a color change. Results are quantified using a standard curve to determine sample concentrations. ELISA offers advantages such as high sensitivity, quantifiability, and being well-suited for individual and mass screening.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 11

    ELISA

    S.SreeDivya

    ELISA

    Enzyme Linked ImmunoSorbent Assay (ELISA) Is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. To determine if an individual is positive for a selected pathogen, such as TB,HIV.

    Types of ELISA

    Qualitative ELISA Postive or Negative results Quantitative ELISA Optical density or fluorescent units of the sample is interpolated into a standard curve, which is typically a serial dilution of the target.

    Different techniques of ELISA

    Direct ELISA

    Indirect ELISA
    Sandwich ELISA

    Competitive ELISA

    ELISA with one Example

    For detection of IgG, IgA and IgM Abs to Mycobacterium sps in human serum. An example for Indirect ELISA

    Components of Elisa
    Microtitre plate: coated with specific Ag Sample Diluent Control Wash buffer Enzyme conjugate: purple in color 2Antibody: Anti-Human IgG/IgA/IgM Ab purified by affinity chromatography. Enzyme: HRP (Horse Radish Peroxidase) Substrate: TMB (TetraMethylBenzidine) -colorless Stop solution: Dilute acid

    Assay Procedure

    Results

    This standard curve is used to determine the unknown concentration of each sample by finding the opposite concentration to the absorbance The quality control sample concentration is determined from the standard curve and if the result is in the range given by the kit manufacturer the results could be accepted.

    Advantages
    detection of antigen (direct ELISA) or antibody (indirect ELISA) very sensitive quantifiable easy to perform high throughput not expensive after developing useful for both individual and mass population screening best method to detect long term infected animals and carrier animals

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