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Curriculum Vitae Provide a brief CV showing education and research training, including any prizes or awards: (no more than one page) Mohammad Riazul Islam, PhD Assistant Professor, Dept. of Biochemistry and Molecular Biology, Faculty of Biological Science, University of Dhaka, Dhaka-1000, Bangladesh Tel: (0880)-2-9661900 ext. 7663 E-mail: riazul_i@yahoo.com Date of Birth: 22 October, 1976
Education: 2006-2009: PhD in Bioscience and Biotechnology, Kyushu University, Japan. 2004-2005: Post Graduate training in Biotechnology, Osaka University, Japan 1998-1999: Master of Science in Biochemistry, University of Dhaka, Bangladesh 1996-1998: Bachelor of Science in Biochemistry, University of Dhaka, Bangladesh Employment: July 2012 Till now Assistant Professor, Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh July 2010- June 2012 JSPS Post-doctoral Research Fellow, Laboratory of Microbial Technology, Faculty of Agriculture, Kyushu University, Japan October 2009- June 2010 Assistant Professor, Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh April 2004 - September 2004 Lecturer, Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh July 2002 April 2004: Research Officer, Tuberculosis Laboratory, International Center for Diarrheal Disease Research, Bangladesh (ICDDRB) Awards and Fellowships: Japan Society for Promotion of Science (JSPS) post-doctoral fellowship (2010-2012) Distinction of Young Scientist in Second International Symposium on Antimicrobial Peptide, Saint-Malo, France, June 2009 MONBUKAGAHUSHO (MEXT, Japan) scholarship for completion of PhD in Kyushu University, Japan, 2006-2009 UNESCO Post Graduate Inter-University Course in Biotechnology, 2004 fellowship, ICBiotech, Osaka University, Japan, 2004-2005. Professional Memberships: Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA) American Chemical Society (ACS) Bangladesh Society for Biochemistry and Molecular Biology (BSBMB) Graduate Biochemist Association (GBA)

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List of Publications in International Journals* * Number all publications in international journals or book chapters, with earliest first and most recent at the end. Provide the Impact Factor (IF) of the journal and indicate your contribution the work described and the publication. Use as many pages as required. Follow the style in these examples:
1. S. Banu , S. V. Gordon, S. Palmer, M. R. Islam, S. Ahmed , K. M. Alam, S. T. Cole, R. Brosch. Genotypic analysis of Mycobacterium tuberculosis in Bangladesh and prevalence of the Beijing strain. Journal of Clinical Microbiology 42, 674-82 (2004) (IF 4.15) In this article, MRIs contribution was in performing the experiments of deletion analysis and MIRU-VNTR analysis for genotyping. 2. M. R. Islam, J. Nagao, K. Shioya, J. Nakayama, K. Sonomoto. Characterization of nukacin ISK-1 biosynthetic enzymes by expressing nukacin ISK-1-lacticin 481 chimeric prepeptides. Annual Report ICBiotech, Osaka University 27, 801-811(2005) In this article, MRI contributed in all parts. 3. J. Nagao, Y. Morinaga, M. R. Islam, S. M. Asaduzzaman, Y. Aso, J. Nakayama, K. Sonomoto. Mapping and identification of the region and secondary structure required for the maturation of the nukacin ISK-1 prepeptide. Peptides 30, 1412-1420 (2009) (IF 2.43) In this article, MRIs contribution was to construction of chimera peptide and their characterization. 4. M. R. Islam, K. Shioya, J. Nagao, M. Nishie, H. Jikuya, T. Zendo, J. Nakayama, K. Sonomoto. Evaluation of essential and variable residues of nukacin ISK-1 by NNK scanning. Molecular Microbiology l72, 14381447 (2009) (IF 5.01) Most of the work in this article was done by MRI. 5. S. Banu, M. K. M. Uddin, M. R. Islam, K. Zaman, T. Ahmed, A. H. Talukder, M. T. Rahman, Z. Rahim, N. Akter, N. Khatun, R. Brosch, H. P. Endtz. Molecular epidemiology of tuberculosis in rural matlab, Bangladesh. International Journal of Tuberculosis and Lung Disease 16, 319-326 (2012) (IF 2.73) In this article MIRU-VNTR and spoligotyping experiments was done by MRI. 6. T. V. Puramattathu, M. R. Islam, M. Nishie, S. Yanagihara, J. Nagao, K. Okuda, T. Zendo, J. Nakayama, K. Sonomoto. Enhanced production of nukacin D13E in Lactococcus lactis NZ9000 by the additional expression of immunity genes. Applied Microbiology and Biotechnology 93, 671-678 (2012) (IF 3.42) In this article the D13E mutant was constructed by MRI and overall supervision of this work was also done by him. 7. M. R. Islam, M. Nishie, J. Nagao, T. Zendo, S. Keller, J. Nakayama, D. Kohda, H-G. Sahl, K. Sonomoto. Ring A of Nukacin ISK-1: A Lipid II-Binding Motif for Type-A(II) Lantibiotic. Journal of American Chemical Society 134, 3687-90 (2012) (IF 9.9) In this article, MRI contributed in most of the parts, as generation of ring A variants, peptidoglycan precursor accumulation experiment and interaction analysis.

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Abstract Indicate below the abstract that is submitted by you for presentation at the YSP and the FAOBMB Congress in Bangkok (include all authors, affiliation(s) and the text of the abstract) Title: Ring A of nukacin ISK-1: a lipid II-binding motif for type-A(II) lantibiotic Mohammad R. Islam1, Mami Nishie1, Jun-ichi Nagao2, Takeshi Zendo1, Jiro Nakayama1, Daisuke Kohda3, Hans-Georg Sahl4 and Kenji Sonomoto1
1

Laboratory of Microbial Technology, Faculty of Agriculture, Kyushu University, Japan; 2Section of Infection Biology, Fukuoka Dental College, Japan; 3Division of Structural Biology, Medical Institute of Bio-regulation, Kyushu University, Japan; 4 Institute of Medical Microbiology, University of Bonn, Germany E-mail: riazul_i@yahoo.com Nukacin ISK-1 is a type-A(II) lantibiotic consisting of 27 amino acids with a linear Nterminal and globular C-terminal region (1). The ring A of nukacin ISK-1, which is also present in different type-A(II) lantibiotics, resembles a lipid II-binding motif (TxS/TxD/EC, x denotes undefined residues) similar to that present in mersacidin (type-B lantibiotics), which suggests that nukacin ISK-1 binds to lipid II as a docking molecule. Results from our experiments on peptidoglycan precursor (UDP-MurNAcpp) accumulation and peptide antagonism assays clearly indicated that nukacin ISK-1 inhibits cell wall biosynthesis accumulating lipid II precursor inside the cell and the peptide activity can be repressed by lipid I and lipid II. Interaction analysis of nukacin ISK-1 and different ring A variants with lipid II revealed that nukacin ISK-1 and nukacin D13E (a more active variant) have a high affinity (KD, 0.17 M and 0.19 M respectively) for lipid II, whereas, nukacin D13A (a less active variant) showed a lower affinity and nukacin C14S (a negative variant lacking the ring A structure) exhibited no interaction. Therefore on the basis of the structural similarity and positional significance of the amino acids in this region, we concluded that nukacin ISK-1 binds lipid II via its ring A region, and may lead to the inhibition of cell-wall biosynthesis (2). Keywords: lantibiotics, nukacin ISK-1, lipid II-binding motif References 1. Asaduzzaman, S. M., Sonomoto, K. (2009) Lantibiotics: diverse activities and unique modes of action. J. Biosci. Bioeng. 107, 475-487 2. Islam, M. R., Nishie, M., Nagao, J., Zendo, T., Keller, S., Nakayama, J., Kohda, D., Sahl, H-G., Sonomoto, K. (2012) Ring A of nukacin ISK-1: a lipid II-binding motif for type-A(II) lantibiotic. J. Am. Chem. Soc. 134, 3687-3690

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Personal Statement Indicate briefly here your Research Interests and Career Goals, why you are interested to participate in the YSP Program (including what you will bring to the YSP and what you hope to gain from it): (no more than one page) After completion of my post-doctoral fellowship from Japan in June 2012, I started working in the Dept. of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh, as an Assistant Professor. In Japan, during my PhD and post-doctoral fellowship, I carried out research on antimicrobial peptide bioengineering and elucidation of their mode of action. I used a model peptide nukacin ISK-1, a ribosomally synthesized and lanthionine containing antimicrobial peptide produced by Gram-positive bacteria and having 27 amino acid residues, for bioengineering to create higher potential peptide variants and to identify the docking molecule for its antimicrobial mechanism. I used site saturation systematic mutagenesis for lantibiotic bioengineering and obtained two high potential peptide variants, that has been published in Molecular Microbiology journal in 2009. I also identified the docking molecule of nukacin ISK-1 as lipid II, during its antimicrobial mechanism and the binding motif of nukacin ISK-1 required for lipid II interaction. We also published this work in Journal of American Chemical Society (JACS) in 2012. Now in Bangladesh, I would like to focus the following things and would like to continue my research in the same field. Screening of potential antimicrobial peptide from the natural sources: Due to the emergence of drug resistant pathogens, it is now inevitable to find out alternative therapeutic agents apart from the conventional antibiotics. In that case, antimicrobial peptides are the promising candidates because of their higher potency and suitable to change the structure by mutagenesis. Therefore, we are planning to search for potential antimicrobial candidate from the nature such as soil, water, plants etc. We will use the conventional procedure for the initial screening. Generation of high potential peptide variants by bioengineering: Using molecular biology technique, we will identify the biosynthetic mechanism of identified peptide. To generate high potential peptide variants, we will perform structure-function study in depth. Elucidation of their antimicrobial mechanism: To identify the antimicrobial mechanism we will investigate the membrane interaction, searching for docking molecule and identification of the binding motif of peptide. Investigation of molecular mechanism for As/Pb metabolism by soil isolated bacteria for As/Pb removal: Arsenic (As) problem in Bangladesh is very critical. This contamination is no longer been in drinking water only; rather it is now speeding in the food chain. We need to find out alternative methods to mitigate this As contamination such as, using microorganisms. Microbes are mostly convenient as they can be easily manipulated. We identified few strains from the As contaminated area and found their higher efficiency for As removal. We will now investigate their mechanism of As metabolism and their use for As removal. Lead (Pb) contamination is also a vital problem in Bangladesh. Most of the industrial areas are heavily contaminated by Pb. Therefore to remove Pb from surface water and soil, we are planning to use microbes that can degrade Pb. We will screen such microbes form the natural sources and will try to investigate the mechanism for Pb degradation. Through joining this congress I can make a good communication with the leading scientists and would like to make some collaboration for my research. I strongly believe that this congress can be a good platform to discuss my current and future research proposals.

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Attachments: Recommendation letter 1

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Recommendation letter 2

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