REPRODUCTION

REVIEW

Focus on Fertilization The mechanism of sperm oocyte fusion in mammals

Keisuke Kaji and Akira Kudo1
Institute for Stem Cell Research, The University of Edinburgh, Roger Land Building, The Kings Building, West Mains Road, Edinburgh, EH9 3JQ, UK and 1 Department of Biological Information, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan
Correspondence should be addressed to Akira Kudo; Email: akudo@bio.titech.ac.jp

Abstract
Sperm oocyte fusion is one of the most impressive events in sexual reproduction, and the elucidation of its molecular mechanism has fascinated researchers for a long time. Because of the limitation of materials and difculties in analyzing membrane protein protein interactions, many attempts have failed to reach this goal. Recent studies involving gene targeting have clearly demonstrated the various molecules that are involved in sperm oocyte binding and fusion. Sperm ADAMs (family of proteins with a disintegrin and metalloprotease domain), including fertilin a, fertilin b and cyritestin, have been investigated and found to be important for binding rather than for fusion and painstaking studies have raised suspicions that their putative receptors, oocyte integrins, are necessary for the sperm oocyte interaction. Recently, several studies have focused the spotlight on CD9 and glycosylphosphatidylinositol (GPI)-anchored proteins on oocytes, and epididymal protein DE on sperm, as candidate molecules involved in sperm oocyte fusion. Lack of, or interference with the function of, these proteins can disrupt the sperm oocyte fusion without changing the binding. In this review we highlight the candidate molecules involved in the sperm oocyte interaction suggested from the recent progress in this research eld.
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Introduction
Fertilization is a complicated process involving many molecules. In mammals, ovulated oocytes are surrounded by two substantial layers; the cumulus cell barrier and the zona pellucida (Fig. 1). Normally, only one sperm penetrates these two layers, and reaches and interacts with the oocyte plasma membrane, resulting in fusion and subsequent formation of a zygote. This process of penetrating these layers is important for sperm not only to reach the oolemma, but also to acquire fusibility. During this process, sperm undergo cellular exocytosis, called the acrosome reaction (Fig. 1). The acrosome is a large, Golgi-derived lysosome-like organelle that overlies the nucleus in the apical region of the sperm head. This organelle secretes enzymes such as the serine protease, acrosin, which helps sperm penetration into the zona pellucida (Abou-Haila & Tulsiani 2000). In addition to the secretion of enzymes, the acrosome reaction brings exposure of the inner acrosomal membrane to the outside by breaking the organelle. This process is necessary for the sperm to acquire fusibility with the oocyte plasma membrane. Spermoocyte fusion begins from the equatorial segment located between the inner acrosomal membrane and the plasma membrane overlying the nucleus in the
q 2004 Society for Reproduction and Fertility ISSN 14701626 (paper) 17417899 (online)

posterior region of the sperm head (Yanagimachi & Noda 1970, Bedford et al. 1979). Oocytes have a unique structure on their surface (Longo & Chen 1984). The surface of oocytes is covered with microvilli with the exception of the region overlying the meiotic spindle. Oocyte microvilli surround the sperm head preceding the spermoocyte fusion. It has been observed that sperm rarely fuse with oocytes at the region lacking microvilli. Therefore, oocyte microvilli, together with the sperm equatorial segment, are thought to be rich in molecules involved in spermoocyte fusion. For a long time, many efforts have been made to nd molecules involved in the process of sperm oocyte fusion. Pioneering studies using anti-sperm mAbs that inhibited fertilization suggested candidate molecules involved in the spermoocyte fusion process. Following such antibody studies came gene targeting, which has proved to be a powerful tool to reveal the function of candidate molecules involved in spermoocyte binding and fusion (Table 1). Although the detailed molecular mechanisms of this process have not yet been claried, recent studies have elucidated certain molecules involved in the fusion or binding reaction (Cuasnicu et al. 2001, Evans 2002). In this review we highlight molecules participating
DOI: 10.1530/rep.1.00163 Online version via www.reproduction-online.org

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Figure 1 Overview of fertilization. Sperm, which has passed the cumlus cell layer, interacts with the zona pellucida (a), and penetrates it (b) with the help of a protease-like enzyme, acrosin, released during the acrosome reaction. Sperm then successfully enter the perivitelline space and make contact with oocyte microvilli. Fusion begins from the sperm equatorial segment (d), and the sperm nucleus is then released into the oocyte cytoplasm (e).

Table 1 Phenotype of oocyte and sperm from knockout mice in sperm oocyte interaction. Molecule Oocyte CD9 GPI-anchor protein (PIG-A) integrin a3 a6 b1 b3 b5 fertilin b cyritestin Binding/fusion normal/reduced , 60%/reduced normal/normal normal/normal normal/normal normal* normal* reduced/, 50% reduced/normal Reference Miller et al. 2000; Miyado et al. 2000; Kaji et al. 2000, 2002 Aleri et al. 2003 He et al. 2003 Miller et al. 2000 He et al. 2003 Hodivala-Dilke et al. 1999 Huang et al. 2000 Cho et al. 1998 Nishimura et al. 2001

Sperm

reduced, more than 80% reduction; %, of control; *, normal number of pups in natural mating.

in the spermoocyte interaction, acting at either the binding or the fusion stage (Fig. 2).

Candidate molecules involved in the sperm oocyte interaction

CD9 on oocytes
CD9 is a member of the ubiquitous tetraspanin family of proteins (also known as tetraspans or transmembrane four superfamily proteins), whose members are characterized by four transmembrane regions and two extracellular loops (Boucheix et al. 1991). The second loop is larger than the rst and has highly conserved amino acid residues (Seigneuret et al. 2001). Although the tetraspanins have no known functional domains common to other protein families, as well as interacting with other tetraspanins they reportedly interact with other proteins including integrins, immunoglobulins, proteoglycans, complement regulatory proteins and growth factor receptors. For this reason, tetraspanins are thought to form multi-molecular complexes with these associated molecules on the plasma membrane, and thus have been implicated in cell adhesion, motility, proliferation, and differentiation (Boucheix & Rubinstein 2001). CD9 has been reported to be composed of a variety of multi-protein complexes involved in various cellular and
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physiological processes, including viral infection in canine and feline cells, muscle cell fusion, cell adhesion and motility (Lofer et al. 1997, de Parseval et al. 1997, Tachibana & Hemler 1999). The rst indication that CD9 is functional in fertilization was obtained from experiments using an antibody against CD9 (Chen et al. 1999). In this study, Chen and colleagues found that the anti-CD9 antibody inhibited the sperm oocyte binding and fusion dose dependently in vitro. Consistent with this nding, CD9 is distributed over the entire oocyte surface except the region overlying the mitotic spindle, which is the only area lacking microvilli on the oocyte surface and where sperm inefciently fuse with oocytes (Kaji et al. 2000). This observed distribution pattern of CD9 corresponds with that found for a6 integrin. This integrin can be a receptor for sperm fertilin, an ADAM (a disintegrin and a metalloprotease domain) family member, and also interacts with CD9 on the egg surface (Miyado et al. 2000, discussed below). We and two other groups reported that CD9-decient female mice were sterile due to a lack of fusibility of their CD9-decient oocytes (Kaji et al. 2000, Le Naour et al. 2000, Miyado et al. 2000). Sperm could bind to oocytes from CD9-decient mice in a similar manner as they could to those from wild-type mice. However, these CD9-decient oocytes rarely fused with sperm either
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Figure 2 Candidate molecules involved in the spermoocyte fusion. Sperm lacking ADAMs shows a severe reduction in their ability to bind to oocytes. Integrins could be a receptor for sperm ADAMs, though no integrin-decient oocytes examined have a defect in either spermoocyte binding or fusion. Lack of GPI-anchored proteins caused by gene targeting of phosphatidylinositol glycan class-A (PIG-A) or by treatment of oocytes with phosphatidylinositolspecic phospholipase C (PI-PLC) causes a reduced sperm binding activity, resulting in severely decreased fusibility. Antibodies against DE become localized at the sperm surface and inhibit the spermoocyte fusion without affecting spermoocyte binding. Oocytes from CD9-decient mice have normal binding ability but severely impaired fusibility with sperm. Because CD9 has the ability to form a multi-molecular complex on the plasma membrane, some other unknown proteins may be involved in the spermoocyte fusion.

in vitro or in vivo. When sperm were injected into oocytes from CD9-decient mice by an intracellular sperm injection (ICSI) technique, the fertilized eggs developed to term, revealing that CD9-decient oocytes could apparently produce zygotes that developed normally (Miyado et al. 2000). Although rare, CD9-decient females bore normal offspring. In the case of in vitro fertilization, the longer incubation time of CD9-decient oocytes with sperm seemed to increase the number of successful fusions. These observations, as well as the fact that tetraspanins can form multi-protein complexes in the plasma membrane to modulate the function of the partners, suggest that CD9 may cooperate with some other molecule(s) to facilitate the sperm oocyte fusion. In connection with the idea of a multi-protein complex, it is also possible that CD9 acts as a linker between the molecules that are functional in the sperm binding and the molecules facilitating fusion. The effects of anti-CD9 antibodies on the spermoocyte binding are not entirely clear. Some reports demonstrated that the antibody inhibited only the spermoocyte fusion (Miller et al. 2000, Miyado et al. 2000, Kaji et al. 2002). In constrast, CD9 was reported to play a role in spermoocyte binding. Zona pellucida-free oocytes treated with anti-CD9 antibodies showed reduced levels of binding to the ligands; fertilin a, fertilin b and cyritestin on sperm when the antibodies were immobilized on small beads (Chen et al. 1999, Takahashi et al. 2001, Wong et al. 2001, Zhu & Evans 2002). Although the functional difference between the lack of CD9 and treatment with anti-CD9 antibodies is not clear, the antibodies may disturb the strength of adhesions mediated by a molecule such as a6 integrin involved in the multi-protein complex together with CD9. In addition to treatment with antibodies, treatment of oocytes with a recombinant form of the large extracellular
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loop (LEL) of CD9 led to a reduction in the sperm oocyte fusion when mouse oocytes were inseminated, though treatment of sperm with this had no effect (Zhu et al. 2002). This nding suggests that interaction between the LEL portion of intact CD9 and some molecule(s) on the oocyte surface was essential for the spermoocyte fusion and that this interaction became unstable due to competition between the recombinant LEL and the endogenous CD9 for interaction with this/these molecule(s). Interestingly, in mice, LEL from mouse CD9 inhibited only fusion, but LEL from human CD9 inhibited spermoocyte binding as well as fusion (Higginbottom et al. 2003). Although it is difcult to explain this difference, it may be possible to identify the regions responsible for the different effects by generating chimeric proteins composed of human LEL and mouse LEL. Oocytes from CD9-decient mice could be rescued by injection with CD9 mRNA (Kaji et al. 2002, Zhu et al. 2002). Using this method, Zhu et al. (2002) determined the functional domains of mouse CD9 acting in the process of sperm oocyte fusion. One point mutation of the amino acid residue 174 (F to A) and triple mutations of the amino acid residues 173175 (SFQ to AAA), in the second loop of CD9, severely reduced the ability of the mutated mRNA to rescue the fusibility of CD9-decient oocytes. The experimental idea that these particular amino acids are important for CD9 function originated from the results of a study showing that the equivalent region of human CD81, also a tetraspanin, was crucial for CD81 binding to hepatitis C virus (HCV, Pileri et al. 1998). When human CD81 functions as an HCV receptor, a phenylalanine at the position of 186 is essential for HCV binding. CD81, and other tetraspanins, of other species lacking phenylalanine at this position also lose this function (Higginbottom et al. 2000). For fertilization,
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human CD9 could restore the ability of CD9-decient mouse oocytes to fuse with sperm and mouse CD81 was able to partially rescue the fusibility, even though the corresponding amino acids of SFQ in mouse CD9 are TFT for human CD9, and TPL for mouse CD81 (Kaji et al. 2002). Because this region in the second loop of the tetraspanins is thought to face to the outside structurally, it is possible that CD9 interacts with other molecules at this region on the oocyte plasma membrane and that this interaction may be essential for efcient fusion (Seigneuret et al. 2001). CD9 has been implicated in other fusion events in addition to fertilization. The addition of anti-CD9 and anti-CD81 mAbs inhibited the conversion of cells of the mouse myoblast cell line (C2C12 cells) to elongated myotubes despite normal expression of muscle-specic proteins. In contrast, ectopic expression of CD9 increased myotube formation (Tachibana & Hamler 1999). CD9/CD81 double-null mice spontaneously developed multinucleate giant cells in their lungs, and showed enhanced osteoclastogenesis in their bones (Takeda et al. 2003). Anti-CD9 antibodies selectively blocked canine distemper virus-induced cell cell fusion (syncytium formation; Schmid et al. 2000). The mechanism(s) of these cellcell fusion events and function of CD9 in such fusion are not known. The role of CD9 in these fusion events is probably not the same as in the sperm oocyte fusion, because the fusion efciency of osteoclasts or multinucleate giant cells in the lungs is increased in CD9-decient mice. These contrasting effects on cellcell fusion may also be explained by the character of tetraspanins, which form unique multi-molecular complexes on individual types of cells.

fertilized and developed normally into two-cell stage embryos, whereas 83% of those from wild-type females were fertilized, and developed normally. Multiple sperm were detected in the perivitelline space of several PIG-A (2 /2 ) oocytes (Aleri et al. 2003), as seen in the mating of CD9-decient females (Miyado et al. 2000). In an in vitro fertilization assay in which zona-free oocytes were used, PIG-A (2 /2 ) oocytes showed a low fertilization rate (, 10%) and lower sperm binding (, 60%) compared with wild-type oocytes. This result suggests that GPI-anchored proteins are more important for the spermoocyte fusion than for the binding. One of the GPI-anchored proteins on oocytes, released by PI-PLC treatment, is CD55 (Aleri et al. 2003), probably detected as a , 70 kDa protein by Coonrod et al. (1999). CD55-decient mice showed normal fertility (Sun et al. 1999). Thus, identication and characterization of the , 35 45 kDa protein, also detected by Coonrod et al. (1999), or still undetected GPI-anchored proteins in lower abundance are needed to elucidate the phenotype of PIGA-decient oocytes, along with further examination of the interaction between this/these protein(s) and CD9.

DE on sperm
One candidate molecule expressed on sperm, which participates in fusion, is epididymal protein DE/cysteine-rich secretory protein 1 (CRISP1). DE was originally identied as a molecule synthesized in rats in an androgen-dependent manner by the proximal segment of the epididymis, and was reported to become localized on the surface of sperm during epididymal transit (Cameo & Blaquier 1976, Kohane et al. 1980a,b). Subsequently, many results indicating its particpation in spermoocyte fusion in rats have been reported. Before the acrosome reaction occurs, DE is localized on the dorsal region of the rat sperm acrosome and subsequently migrates to the equatorial segments, where sperm begins to fuse with an oocyte (Rochwerger & Cuasnicu 1992). Polyclonal anti-DE antibodies signicantly prevented sperm from fusing with zona-free oocytes without changing either the mobility or binding ability of the sperm (Cuasnicu et al. 1984). A puried DE protein could bind to the entire surface of oocytes except the region over the meiotic spindle, where sperm rarely fuse (Rochwerger et al. 1992), suggesting that there is a receptor for DE on the surface of oocytes for promoting the sperm oocyte fusion. The mouse epididymal protein, AEG-1/CRISP-1, possesses 70% homology to rat DE. Proteins recognized by anti-rat DE antibodies are localized on the dorsal region of the sperm acrosome, although it is not clear whether they are AEG-1 or not and mouse oocytes also have rat DE-binding sites similar to those of rat oocytes (Mizuki & Kasahara 1992, Cohen et al. 2000). Furthermore, anti-rat DE antibodies reduced the penetrability of mouse sperm without reducing their ability to bind to mouse oocytes (Cohen et al. 2000). The human epididymal protein
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Glycosylphosphatidylinositol (GPI)-anchored proteins on oocytes

In addition to CD9-decient oocytes, oocyte-specic phosphatidylinositol glycan class-A (PIG-A)-decient mice also showed severely reduced fusibility of oocytes with sperm (Aleri et al. 2003). The PIG-A gene encodes a subunit of N-acetyl glucosaminyl transferase, which is the enzyme involved in the rst step of the biosynthesis of the GPI-anchor (Tiede et al. 2000). Participation of oocytespecic GPI-anchored proteins in the sperm oocyte interaction was suggested earlier in a study in which oocytes were treated with phosphatidylinositol-specic phospholipase C (PI-PLC; Coonrod et al. 1999). This caused release of two GPI-anchored proteins (, 35 45 kDa and 70 kDa), and a reduction in the sperm oocyte binding and strong blockage of the spermoocyte fusion. Complete deciency of PIG-A resulted in embryonic lethality (Kawagoe et al. 1996), so oocyte-specic PIG-A-decient mice were generated by use of the Cre-loxP system, in which Cre-recombinase was expressed under control of the oocyte-specic promoter ZP3. After natural mating, only 1.3% of the recovered healthy cells were successfully
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ARP/hCRISP-1, showing 40% homology to rat DE, is also found on human sperm and exposure of human sperm to anti-ARP antibodies signicantly reduced the penetrability of the sperm into hamster oocytes without changing sperm viability, motility or binding, thus making this antibody useful for evaluating the ability of human sperm to fuse with oocytes (Yanagimachi et al. 1976, Cohen et al. 2001).

Integrins on oocytes and ADAMs on sperm

Before the discovery of a functional role for CD9 in the spermoocyte fusion, most attention had been given to integrins on oocytes and their ligands, fertilin a, fertilin b and cyritestin, on sperm. Fertilin a, fertilin b and cyritestin are members of the ADAM family, being ADAM1, ADAM2, and ADAM3, respectively, each of which has a disintegrin and a metalloprotease domain. Originally fertilin b was identied as the antigen recognized by the mAb, PH-30, an anti-sperm antibody that inhibits fertilization of guinea pig oocytes. and was found to form a heterodimer with fertilin a (Blobel et al. 1992), whereas cyritestin plays a role as a single polypeptide (Nishimura et al. 2001). Fertilin a has an amino acid sequence called the fusion peptide in addition to a disintegrin domain that can bind to integrins on ooctytes. The fusion peptide fullls the following criteria for such a peptide: (a) location in a membrane-anchored subunit, (b) relatively strong hydrophobicity, and (c) ability to be modeled as a sided a-helix with most of the bulky hydrophobic residues on one face and charged amino acids on the other face. These are the same characteristics of viral fusion peptides involved in the penetration of the target cell membrane preceding fusion events between cells and virus particles (de Parseval et al. 1997). Therefore, it is likely that sperm ADAMs play a key role in the spermoocyte binding and fusion. ADAM functions have been examined by use of in vitro fertilization in combination with antibodies, peptides and isolated proteins. Recombinant forms of fertilin a, fertilin b and cyritestin were shown to bind to the mouse oocyte plasma membrane and inhibit the spermoocyte binding, resulting in a reduced incidence of fertilization (Evans et al. 1997b, Takahashi et al. 2001). In addition to the data obtained in vitro, knockout mice specic for these genes have provided evidence for the participation of the sperm ADAMs in the spermoocyte interaction. For example, sperm from fertilin b-decient mice were severely inhibited (, 80%) in their oolemma-binding ability, though some of the few sperm that could bind were able to fuse with the oocyte (Cho et al. 1998). Cyritestinnull sperm also demonstrated a great reduction in their ability to bind to oocytes in spite of a having normal spermoocyte fusion (Nishimura et al. 2001). Interestingly, fertilin b-decient sperm lacked fertilin a, and showed a greatly reduced amount of cyritestin, whereas cyritestin-decient ones lacked fertilin a and showed
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reduced fertilin b expression, , 50% compared with the wild-type sperm (Nishimura et al. 2001). Although fertilin a-decient mice, and their associated levels of fertilin b and cyritestin expression, have not been reported yet, these 3 ADAMs appear to play important roles in the sperm oocyte binding but not in the fusion. Besides sperm ADAMs, their putative receptors on oocytes, i.e. integrins, have also been well studied as candidate genes being responsible for the sperm oocyte interaction. The rst member of the integrin family shown to be active in this regard was a6b1. GoH3, a functionblocking mAb against the a6 integrin subunit, inhibited both sperm oocyte binding and fusion, and peptides derived from a sequence of a part or all of the fertilin b disintegrin domain bound to a6b1 integrin (Almeida et al. 1995, Chen & Sampson 1999, Takahashi et al. 2000). However, the contribution of a6b1 integrin to the sperm oocyte interaction remains questionable because some inhibition studies using GoH3 mAb under different conditions did not show blocking of the interaction (Evans et al. 1997a, Miller et al. 2000). Moreover, oocytes from a6 integrin-decient mice showed normal binding and fusibility with sperm, demonstrating that the a6 integrin is not essential for the gamete binding and fusion (Miller et al. 2000). This result evoked the possibility that the function of a6 integrin is redundant, because some other members of the integrin family were also expressed on oocytes. Integrins expressed on the surface of mouse oocytes can be divided into 2 groups: b1 integrins (a2b1, a3b1, a5b1, a6b1 and a9b1) and av integrins (avb1, avb3, avb5; He et al. 2003). In addition to a6 integrindecient mice, a3-, b3- and b5-decient mice were also reported to show normal fertility (Hodivala-Dilke et al. 1999, Huang et al. 2000, He et al. 2003). Because these results could not exclude the possibility of the occurrence of functional redundancy among various integrins, He et al. (2003) performed some elaborate experiments. As b1 integrin-decient mice were preimplantation lethal, they established oocyte-specic b1 integrin-decient mice by use of the Cre-loxP system. The b1 conditionally decient females showed the normal pregnancy rate, and their oocytes demonstrated normal binding and fusion with sperm in an in vitro fertilization assay. Moreover, in the presence of anti-b3 or av integrin antibodies for functional blocking, oocytes showed normal sperm binding and fusibility. Thus, integrins on mouse oocytes are unlikely to be redundant with each other in the sperm oocyte binding and fusion.

Conclusions and perspectives

Because of the limitation of materials and difculties in analyzing membrane proteinprotein interactions, the molecular mechanism of sperm oocyte fusion remains to be elucidated, even though it is one of the most important events as it triggers the beginning of development. Advanced studies on virus infection and exocytosis
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K Kaji & A Kudo Cameo MS & Blaquier JA 1976 Androgen-controlled specic proteins in rat epididymis. Journal of Endocrinology 69 47 55. Chen H & Sampson NS 1999 Mediation of sperm-egg fusion: evidence that mouse egg alpha6beta1 integrin is the receptor for sperm fertilinbeta. Chemistry and Biology 6 1 10. Chen MS, Tung KS, Coonrod SA, Takahashi Y, Bigler D, Chang A, Yamashita Y, Kincade PW, Herr JC & White JM 1999 Role of the integrin-associated protein CD9 in binding between sperm ADAM 2 and the egg integrin alpha6beta1: implications for murine fertilization. PNAS 96 1183011835. Cho C, Bunch DO, Faure JE, Goulding EH, Eddy EM, Primakoff P & Myles DG 1998 Fertilization defects in sperm from mice lacking fertilin beta. Science 281 18571859. Cohen DJ, Ellerman DA & Cuasnicu PS 2000 Mammalian sperm-egg fusion: evidence that epididymal protein DE plays a role in mouse gamete fusion. Biology of Reproduction 63 462468. Cohen DJ, Ellerman DA, Busso D, Morgenfeld MM, Piazza AD, Hayashi M, Young ET, Kasahara M & Cuasnicu PS 2001 Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg. Biology of Reproduction 65 10001005. Coonrod SA, Naaby-Hansen S, Shetty J, Shibahara H, Chen M, White JM & Herr JC 1999 Treatment of mouse oocytes with PIPLC releases 70-kDa (pI 5) and 35- to 45-kDa (pI 5.5) protein clusters from the egg surface and inhibits sperm-oolemma binding and fusion. Developmental Biology 207 334349. Cuasnicu PS, Gonzalez Echeverria F, Piazza AD, Cameo MS & Blaquier JA 1984 Antibodies against epididymal glycoproteins block fertilizing ability in rat. Journal of Reproduction and Fertility 72 467 471. Cuasnicu PS, Ellerman DA, Cohen DJ, Busso D, Morgenfeld MM & Da Ros VG 2001 Molecular mechanisms involved in mammalian gamete fusion. Archives of Medical Research 32 614 618. de Parseval A, Lerner DL, Borrow P, Willett BJ & Elder JH 1997 Blocking of feline immunodeciency virus infection by a monoclonal antibody to CD9 is via inhibition of virus release rather than interference with receptor binding. Journal of Virology 71 57425749. Evans JP 2002 The molecular basis of sperm-oocyte membrane interactions during mammalian fertilization. Human Reproduction Update 8 297 311. Evans JP, Kopf GS & Schultz RM 1997a Characterization of the binding of recombinant mouse sperm fertilin beta subunit to mouse eggs: evidence for adhesive activity via an egg beta1 integrinmediated interaction. Developmental Biology 187 7993. Evans JP, Schultz RM & Kopf GS 1997b Characterization of the binding of recombinant mouse sperm fertilin alpha subunit to mouse eggs: evidence for function as a cell adhesion molecule in spermegg binding. Developmental Biology 187 94106. He ZY, Brakebusch C, Fassler R, Kreidberg JA, Primakoff P & Myles DG 2003 None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion. Developmental Biology 254 226237. Higginbottom A, Quinn ER, Kuo CC, Flint M, Wilson LH, Bianchi E, Nicosia A, Monk PN, McKeating JA & Levy S 2000 Identication of amino acid residues in CD81 critical for interaction with hepatitis C virus envelope glycoprotein E2. Journal of Virology 74 36423649. Higginbottom A, Takahashi Y, Bolling L, Coonrod SA, White JM, Partridge LJ & Monk PN 2003 Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion. Biochemical and Biophysysical Research Communications 311 208 214. Hodivala-Dilke KM, McHugh KP, Tsakiris DA, Rayburn H, Crowley D, Ullman-Cullere M, Ross FP, Coller BS, Teitelbaum S & Hynes RO 1999 Beta3-integrin-decient mice are a model for Glanzmann thrombasthenia showing placental defects and reduced survival. Journal of Clinical Investigation 103 229238.
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demonstrate that molecules having a helix-hemagglutinin (HA) in the viral membrane and SNAREs in intracellular vesicles, etc. play a key role to bring the two plasma membranes into extremely close proximity preceding fusion (Blumenthal et al. 2003). However, the specic docking molecules used to achieve the stable association between the two membranes have not been identied in any cell cell fusion events, such as myotube formation, osteoclastogenesis, giant cell formation, as well as fertilization. The phenotype of CD9-decient oocytes shows a quite severe abnormality in the fusion process with sperm. However, it is not likely that CD9 is the docking molecule by itself, because all cells expressing CD9 in various tissues, except oocytes, cannot fuse with sperm. Moreover, even if oocytes lack CD9, in rare cases some of them can still fuse with sperm. Thus, identication of oocytespecic CD9-associating molecules might help to clarify the mechanism of sperm oocyte fusion. Furthermore, prior to dening such associating molecules, identication and characterization of the GPIanchored , 35 45 kDa protein, a receptor for epididymal protein DE on oocytes, is needed. Further studies including the generation of knockout mice specic for these genes and analysis of the interaction between these molecules should help us in clarifying the molecular mechanisms of both binding and fusion aspects of the spermoocyte interaction.

Acknowledgements
We thank Dr Brian Hendrich for critical reading of and comments on the manuscript.

References
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