This document contains 18 multiple choice and short answer questions about fungal morphology, culture, and identification. It tests knowledge of microscopic fungal structures, growth characteristics, staining techniques, and specimen collection for mycology testing. The questions would be used to assess understanding of basic mycology concepts for microbiology trainees.
This document contains 18 multiple choice and short answer questions about fungal morphology, culture, and identification. It tests knowledge of microscopic fungal structures, growth characteristics, staining techniques, and specimen collection for mycology testing. The questions would be used to assess understanding of basic mycology concepts for microbiology trainees.
This document contains 18 multiple choice and short answer questions about fungal morphology, culture, and identification. It tests knowledge of microscopic fungal structures, growth characteristics, staining techniques, and specimen collection for mycology testing. The questions would be used to assess understanding of basic mycology concepts for microbiology trainees.
Rotation II Basics of Mycology Case Studies & Study Questions
Basics of Mycology Study Questions
1. True or False. Fungi resemble plants in that both contain chlorophyll.
2. True or False: Arthroconidia reproduce by fragmentation.
3. The two growth forms of fungi include: a. Yeast and hyphae b. Yeast and mould c. Blastoconidia and mould d. Conidia and hyphae
4. A mass of branching intertwined hyphae forming a matt of growth is known as: a. Hyphae b. Conidia c. Mycelium d. Favic chandeliers
5. Blastoconidia that have elongated are known as: a. Hyphae b. Pseudohyphae c. Arthroconidia d. Racquet hyphae
6. The India ink stain is used to detect what part of a yeast cell? a. Chlamydoconidia b. Pseudohyphae c. Blastoconidia d. Capsule
7. A fungus that has a yeast phase that grows at 35C and a mold phase that grows at 30C is referred to as: a. Dimorphic b. Polymorphic c. Bimorphic d. Anamorphic
8. The purpose for using a KOH preparation when doing a direct examination for fungi is to: a. Dissolve the keratin to unmask the fungus elements b. Stain the hyphae and conidia of the fungi c. Reveal capsules that may be found around yeast cells d. Kill any bacteria that may be present in the specimen
9. In the Lactophenol aniline blue stain, what reagent stains the chitin in the fungal cell wall? a. Aniline blue b. Phenol c. Lactic acid d. Glycerol
10. What is the atmospheric requirement for fungi to grow? a. Anaerobic b. Ambient air c. 5-10% CO 2
d. >10% CO 2
CLS419 Clinical Microbiology II Mycology Page 20 Rotation II Basics of Mycology Case Studies & Study Questions 11. Of the following techniques, which one is the best for maintaining the juxtaposition of the fungal elements and can be kept in the laboratory permanently? a. Tease preparation b. Scotch tape preparation c. Slide culture
12. Which of the following approaches is NOT used in the identification of fungi? a. Growth rate b. Colony morphology c. Microscopic appearance d. Methylene blue stain
13. What is/are the benefits to performing a direct mount examination on every specimen submitted for fungal culture? a. Allows you to send out an immediate preliminary report to the primary care provider so they can initiate treatment or look for other diagnoses b. Enables you to know if special media needs to be inoculated c. Allows you to observe the yeast phase of dimorphic organisms d. May provide a clue as to the identity of the causative agent without having to wait for the fungus to incubate e. All of the above
14. Match the media with its general use (answers can be used more than once and items can have more than one answer):
_____ Mycosel agar a. Nonselective, primary isolation media b. Demonstrate phenol-oxidase enzyme _____ Sabourauds Dextrose agar c. Promotes blastoconidia, chlamydospores and hyphal formation _____ Brain Heart Infusion agar d. Selective media e. Stimulates spore formation and pigmentation _____ Cornmeal Tween 80 agar f. Used to demonstrate yeast phase of dimorphic fungi _____ Inhibitory Mold agar
_____ Niger Seed agar
_____ Potato Dextrose agar
15. Match the image with its descriptive term.
_____ Mycelium
_____ Nodular organ
_____ Spiral hyphae
_____ Racquet hyphae
_____ Favic chandelier a.
b.
c. d.
e.
CLS419 Clinical Microbiology II Mycology Page 21 Rotation II Basics of Mycology Case Studies & Study Questions 16. Match the picture of the asexual structure with its descriptive term.
_____ Blastoconidia
_____ Arthroconidia
_____ Macroconidia
_____ Microconidia
_____ Phialoconidia
_____ Sporangiospores
a.
b.
c.
d.
e.
17. List three general rules for good collection of fungal specimens.
18. A nurse calls you and asks how to collect a toenail sample for a fungal culture. Give complete instructions that you would give to the nurse. Then describe a basic outline of what process you as a clinical laboratory scientist will follow relating to direct examination, specimen processing and fungal culture setup when you receive the specimen in the lab.