7/16/2014 Extraction of Trigonella foenum-graecum L.

by supercritical fluid CO2 and its contact toxicity to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichid
http://link.springer.com/article/10.1007/s10340-007-0167-8 1/6
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Journal of Pest Science
August 2007, Volume 80, Issue 3, pp 151-157
Extraction of Trigonella foenum-graecum L. by supercritical
fluid CO
2
and its contact toxicity to Rhyzopertha dominica
(Fabricius) (Coleoptera: Bostrichidae)
Abstract
Rhyzopertha dominica (Fabricius) has developed extensive pesticide resistance in the last several decades. We
have developed a supercritical fluid extraction method for Trigonella foenum-graecum L. (TFG) and studied
the contact toxicities of the extracts to R. dominica. The extraction method was designed with orthogonal
experiments to preserve and collect all the possible active components. Contact toxicity and efficiency of
extraction were used as standard values to optimize extraction conditions, which were achieved at 55C under
25 Mpa of pressure. The extraction efficiency for 200 g of dry sample reached 6.21% with 30 ml of 95%
alcohol. Extracts loaded on filter paper showed dose and time dependent toxicities to adult R. dominica with a
LC
50
value of 65.02 g/cm
2
after 3 days post treatment. Our extensive in vivo studies indicated the extracts from
Trigonella foenum-graecum seeds have high efficacy against pesticide resistant R. dominica. The active
ingredient(s) from the extract shows promise as a novel pesticide candidate.
1 Citation
Within this Article
1. Introduction
2. Materials and methods
3. Results
7/16/2014 Extraction of Trigonella foenum-graecum L. by supercritical fluid CO2 and its contact toxicity to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichid
http://link.springer.com/article/10.1007/s10340-007-0167-8 2/6
4. Discussion and conclusion
5. References
6. References
7/16/2014 Extraction of Trigonella foenum-graecum L. by supercritical fluid CO2 and its contact toxicity to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichid
http://link.springer.com/article/10.1007/s10340-007-0167-8 3/6
Related Content
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7/16/2014 Extraction of Trigonella foenum-graecum L. by supercritical fluid CO2 and its contact toxicity to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichid
http://link.springer.com/article/10.1007/s10340-007-0167-8 4/6
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About this Article
Title
7/16/2014 Extraction of Trigonella foenum-graecum L. by supercritical fluid CO2 and its contact toxicity to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichid
http://link.springer.com/article/10.1007/s10340-007-0167-8 5/6
Extraction of Trigonella foenum-graecum L. by supercritical fluid CO
2
and its contact toxicity to
Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae)
Journal
Journal of Pest Science
Volume 80, Issue 3 , pp 151-157
Cover Date
2007-08-01
DOI
10.1007/s10340-007-0167-8
Print ISSN
1612-4758
Online ISSN
1612-4766
Publisher
Springer-Verlag
Additional Links
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About This Journal
Manuscript Submission
Topics
Forestry
Ecology
Plant Sciences
Plant Pathology
Agriculture
Keywords
Trigonella foenum-graecum L.
Supercritical extraction of CO2
Orthogonal experiment
Rhyzopertha dominica (Fabricius)
Contact toxicity
7/16/2014 Extraction of Trigonella foenum-graecum L. by supercritical fluid CO2 and its contact toxicity to Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichid
http://link.springer.com/article/10.1007/s10340-007-0167-8 6/6
Industry Sectors
Chemical Manufacturing
Authors
G. W. Tang
(1)
C. J. Yang
(1)
L. D. Xie
(2)
Author Affiliations
1. College of Plant Science and Technology, Huazhong Agricultural University, 430070,
Wuhan, Hubei, Peoples Republic of China
2. College of Food Science and Engineering, Wuhan Polytechnic University, 430023,
Wuhan, Hubei, Peoples Republic of China
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