In lymphedema, the microcirculation in the affected area of the body is disrupted. Lymphatics were mentioned more than 2000 years ago by Aristotle. The discovery of the lymphatics is attributed to the Italian anatomist Gasparo Aselli.
In lymphedema, the microcirculation in the affected area of the body is disrupted. Lymphatics were mentioned more than 2000 years ago by Aristotle. The discovery of the lymphatics is attributed to the Italian anatomist Gasparo Aselli.
In lymphedema, the microcirculation in the affected area of the body is disrupted. Lymphatics were mentioned more than 2000 years ago by Aristotle. The discovery of the lymphatics is attributed to the Italian anatomist Gasparo Aselli.
Failure of the lymphatic system to transport lymph from the
interstitial space back to the bloodstream results in lymphatic stasis. If the collateral lymphatic circulation is insufcient and all compensatory mechanisms are exhausted, the protein-rich interstitial uid accumulates and lymphedema develops. In lymphedema, caused by either congenital or acquired dys- function of the lymphatic system, the microcirculation in the affected area of the body is disrupted. The transport of the excess tissue uid containing lymphocytes, different plasma proteins, immunoglobulins, and cytokines is impaired and chronic inammatory changes in the subcutaneous tis- sue and skin develop. Progress in ultrastructural, cytochemi- cal, and imaging studies and improvement in conservative and surgical treatment of lymphedema have stimulated substantial interest in lymphatic disease. Historical background Lymph vessels were mentioned more than 2000 years ago by Aristotle, who described nerves which contain colorless liquids and later by members of the Alexandrian School of Medicine, who recognized arteries in the mesentery full of milk. This knowledge, however, was lost during the Middle Ages, and it was only in the Renaissance that attention was fo- cused again on the lymphatic system. The thoracic duct was observed in 1563 by Eustachius, who called it vena alba thoracis. He failed, however, to recognize the function of the thoracic duct and its relation to the lymphatic system. The discovery of the lymphatics is attributed to the Italian anatomist Gasparo Aselli, who in 1622 observed the mesen- teric lymphatics in a well-fed dog. He also recognized the function of the lacteals, although he suggested mistakenly that the chyle absorbed from the intestine by the mesenteric lym- phatics was transported to the liver. In 1651, Pecquet described the thoracic duct and recognized the correct route of lym- phatic transport from the mesenteric lymphatics through the receptaculum chyli and the thoracic duct to the subclavian vein. Further details on the anatomy of the lymphatic system were published in the 17th century by Bartholin and Rudbeck, and by the great anatomists of the 18th century, Mascagni and Cruikshank. It was most likely William Hunter who recog- nized the lymphatics as a separate system responsible for absorption. Although Hunter suggested that the lymphatics were closed tubes, one of his students, Hewson, recognized that they had physiologic orices, which, like capillary tubes sucked up tissue uid. It was not until the turn of the 20th century, however, that Starling conrmed the relationship between the oncotic pressure of the plasma proteins and the hydrostatic pressure in the capillaries. 1,2 Starling suggested that lymph formed by ltration of the blood through the capil- lary walls. Drinker, 3 and later Rusznyk and colleagues, 4 de- serve the credit for clarifying the details of protein absorption from the intercellular space via the lymphatic system. Interest in lymphatic diseases was greatly enhanced by Kinmonth, who described a clinically usable technique of direct contrast lymphangiography in 1952. 5 Improvement in other imaging techniques, such as lymphoscintigraphy, 6,7 indirect lymphan- giography, 8,9 and magnetic resonance imaging, 1012 furthered the understanding of the structure and function of the lym- phatic system in different lymphatic disorders. Progress in conservative management 13,14 and development of micro- surgical operations on the lymph vessels 1517 also have stimu- lated experimental and clinical research in lymphatic diseases. Development of the lymphatic system The lymphatic system is rst apparent in the human fetus at 6 weeks of gestation, and it consists of paired jugular, iliac, and retroperitoneal lymph sacs (Fig. 18.1). 18 The origin of the lym- phatic system is controversial, but it is most likely a derivative from the venous system. Another possible theory is that it develops independently of the veins from the mesenchymal tissue. The lymph vessels grow from the paired primitive lymphatic sacs and coalesce along the major veins to form the afferent vessels, nodes, and efferent lymphatic ducts. The Physiologic changes in lymphatic dysfunction Peter Gloviczki 18 Vascular Surgery: Basic Science and Clinical Correlations, Second Edition Edited by Rodney A. White, Larry H. Hollier Copyright 2005 Blackwell Publishing cisterna chyli develops from one of the large retroperitoneal lymph sacs, whereas the other forms the mesenteric lymphat- ic system. There are paired thoracic ducts in the embryo, and the mature thoracic duct develops from fusion of the upper portion of the left and the lower portion of the right thoracic duct. The right cervical lymphatic duct is formed by the right jugular lymphatic sac. This receives lymph from the right face, neck, and the right upper extremity, and from the upper part of the right thorax and mediastinum. Abnormalities in the devel- opment of the lymphatic system include agenesis, hypoplasia, or hyperplasia of the lymphatics with valvular incompetence. They may result in lymphedema or in abnormalities in the circulation of the chyle, such as chylous ascites, chylothorax, reux of chyle to the pelvis or lower extremities, or protein- losing enteropathy. Persistence of some of the embryonic sacs may cause the development of lymphatic cysts, which may or may not communicate with the lymphatic system. Anatomy of the lymphatic system The adult lymphatic system consists of peripheral lymph ves- sel, lymph nodes, and major lymphatic trunks. The peripheral lymph vessels collect lymph from the lymphatic capillaries, which absorb a portion of the interstitial uid from the inter- stitial space. Afferent lymph channels transport lymph to the lymph-conducting elements of the lymph nodes, which lter and further conduct the lymph uid to efferent lymphatic channels. Signicant communications between the lymphatic and venous system in lymph nodes normally do not exist. Eighty percent of the lower extremity lymph is carried by the supercial lymphatic system. Although there is a lateral supercial bundle located around the lesser saphenous vein, most of the lower extremity lymph is transported by lymph channels of the supercial medial bundle (Fig. 18.2). There is a deep lymphatic network that runs in close proximity to the tibial and peroneal vessels and transports lymph through the popliteal lymph nodes into the deep femoral lymphatics. The supercial and the deep lymphatics join in the inguinal lymph nodes and drain lymph toward the aortoiliac lym- phatic system. The cisterna chyli is located between the aorta and the inferior vena cava, usually at the level of L1 to L2. Mesenteric lymphatics join the lower extremity and pelvic lymphatics at this level and drain through the thoracic duct to the left subclavian vein (Fig. 18.3). A very small amount of mesenteric lymph is drained toward the liver around the he- patic vein and the diaphragm to the mediastinal lymphatics. The upper extremity lymphatics run along the major veins of the arm. Although the medial arm bundle is the most signif- icant route of lymph drainage in normal patients, after axillary node dissection lymph is drained primarily through the lateral lymphatic bundle to the deltoideopectoral and supra- clavicular nodes (Fig. 18.4). Asingle layer of endothelial cells forms the inner layer of the lymphatic capillaries. Basal membranes similar to blood capil- laries are not present. The lymphatic capillaries contain bicuspid lymphatic valves, which play a crucial role in the initial lymphatic transport and are responsible for the unidi- rectional lymphatic ow. The capillaries are anchored by small microbrils that expand the endothelial cells and increase the lumen of the capillaries if the tissue pressure is elevated. 19,20 Although smaller molecules may traverse the lymphatic PART I Vascular pathology and physiology 208 A B C Figure 18.1 Development of the lymphatic system. (A) Seven-week embryo with paired iliac, retroperitoneal, and jugular lymph sacs. (B) At 9 weeks of gestation, paired thoracic ducts are present with numerous connections across the midline. (C) Portions of both primitive thoracic ducts persist to form the thoracic duct in the adult. The right lymphatic duct is formed from the primitive right jugular lymphatic sac. (From Cambria RA, Gloviczki P. Lymphedema: pathophysiology and management. In: Callow AD, ed. Vascular Surgery. Norwalk, CT: Appleton & Lange, 1995:1593.) CHAPTER 18 Physiologic changes in lymphatic dysfunction 209 endothelial cells with active phagocytosis, large molecules enter through the gaps between the endothelial cells of the lymphatic capillary. Lymphatic physiology According to Starlings law, hydrostatic and osmotic pressures in the capillaries and in the interstitial space determine the amount of interstitial uid that is ultraltered from the blood plasma. Additional factors responsible for interstitial uid exchange include capillary permeability, the number of active capillaries, the ratio of precapillary arteriolar to postcapillary venular resistance, and the total extracellular uid volume. The amount of uid that moves across the capillary wall is tremendous, considering that the cardiac output is about 8000 l during a 24-h period. It is likely that an amount equal to the total plasma volume enters the interstitial place and leaves through the venous end of the capillaries and the lymphatics every minute. 21 The lymphatic system is responsible for the transport of 24 l of interstitial uid daily. During the same time, approximately 100g of plasma protein is carried back to the circulation by the lymphatics. 22 The protein content of the lymph is somewhat less than that of the plasma, and lymph vessels from various parts of the body contain different amounts of protein (Table 18.1). The lymphatic capillaries are able to transport large molecules, even those with a molecular weight over 1kDa. 23 Figure 18.2 Anatomy of major lymph vessels and lymph nodes of the lower extremity. (From Gloviczki P. Microsurgical treatment for chronic lymphedema: an unfullled promise? In: Bergan JJ, Yao JST, eds. Venous Disorders. Philadelphia: WB Saunders, 1990:344.) Figure 18.3 Anatomy of the thoracic duct. (From Gloviczki P, Noel AA. Lymphatic reconstructions. In: Rutherford RB, ed. Vascular Surgery, 5th edn. Philadelphia: WB Saunders, 2000:2159, with permission from Elsevier.) The single most important determinant of lymph ow through the lymphatic capillaries and the collecting lymph vessels is the intrinsic contractility of the lymph vessels. In addition, lymph ow in inuenced by increased interstitial pressure, muscular activity, arterial pulsation, respiratory pressure, and gravity. Increase in interstitial volume and inter- stitial pressure results in opening of the gaps between the endothelial cells of the terminal lymphatics and an increase in lymphatic transport. Because the endothelial cells contain actin and are able to contract actively, contraction of terminal lymphatics with the help of competent valves enables rapid lymphatic transport. Intrinsic contractility of the smooth muscle in larger collecting vessels allows further propulsion of the lymph. Strength and frequency of the contractions are greatly inuenced by changes in intraluminal pressure. 24 Adrenergic stimulation 25 and endothelin 26 also have been shown to result in contraction of the lymph vessels. Patent blue dye injected into the subcutaneous tissue is transported centrally in the lymph vessels at the rate of 45mm/s, even without any muscular exercise. Intrinsic contractions of the lymph vessel wall with competent valves are able to propel lymph intermittently against a pressure as high as 50mmHg. The major difference that distinguishes the lymphatic sys- tem from the venous system is that the veins are lled with a continuous liquid column. The lymphatic system, however, is not fully primed, and only if there is longstanding stasis does the lymph column ll the lymphatic channels complete- ly. 23 It is only in these conditions that muscular contraction or external massage play an important role in forward propul- sion of the lymph and facilitate lymphatic transport. Pathophysiology of lymphedema Lymphedema develops when the lymphatic load exceeds the transport capacity of the lymphatic system. In patients with lymphatic obstruction, numerous compensatory mechanisms develop. These include collateral lymphatic circulation, de- velopment of spontaneous lymphovenous anastomoses, and increased activity of tissue macrophages to split macro- molecules in the interstitial space, enabling them to be reab- sorbed through the venous end of the capillaries (Fig. 18.5). If the lymphatic transport is impaired due to injury or ob- struction to the lymph vessels and lymph nodes, the different compensatory mechanisms can function effectively for a period of time. This explains why chronic lymphedema of the limbs may develop several months or even years after an edema-free state after inguinal or axillary node dissection or irradiation. Lymphedema is a high-protein edema that, except very early in the course of the disease, is nonpitting in nature (Fig. 18.6). Without treatment, the high-protein edema uid in the subcutaneous tissue will be replaced by brous material, in- ammatory cells accumulate, and progressive brosis of the subcutaneous tissue and skin develops. Fibrosis of the lymph vessels leads to loss of permeability and loss of intrinsic con- tractility. Dilation of the lymph vessels causes valvular incom- petence, and the inammatory and brotic changes destroy the valve leaets, further decreasing the transport capacity of the lymphatic system. Microsurgical reconstruction in this late stage of lymphedema, using brotic and incompetent lymphatics, cannot restore normal lymphatic transport. Progression of lymphedema results in brotic obstruction of PART I Vascular pathology and physiology 210 Table 18.1 Approxiamte protein content of lymph in humans* Lymph origin Protein content (g/dl) Ankle 0.5 Limbs 2 Intestine 4 Liver 6 Thoracic duct 4 *Data based on various studies in humans and animals. (From Ganong WF. Review of Medical Physiology, 10th edn. Los Altos, CA: Lange Medical Publications, 1981: 452.) Figure 18.4 Anatomy of major lymph vessels and lymph nodes of the upper extremity. (From Gloviczki P. Microsurgical treatment for chronic lymphedema: an unfullled promise? In: Bergan JJ, Yao JST, eds. Venous Disorders. Philadelphia: WB Saunders, 1990:344.) Figure 18.5 Stages in development of postsurgical lymphedema. (Modied from Gloviczki P, Schirger A. Lymphedema. In: Spittell JA, ed. Clinical Medicine. Philadelphia: Harper & Row, 1985:1.) Figure 18.6 Chronic secondary lymphedema of the left lower extremity in a 47-year-old man after iliac node dissection, followed by irradiation. the lymph nodes and the major lymph vessels. Even the larger lymphatic collaterals, which functioned effectively in the ini- tial period after lymphatic obstruction, may occlude with time. In this stage, dilated dermal lymphatics provide the only lymphatic drainage of the extremity. Using noninvasive func- tional tests, such as radionuclide lymphoscintigraphy per- formed with technetium-labeled antimony sulfur colloid, it is possible to repeat the studies in the same patient and docu- ment progression of the disease (Fig. 18.7). Lymphatic stasis also results in deciency of important im- munoglobulins, cytokines, and plasma proteins. Because of chronic inammatory changes in the subcutaneous tissue and the skin, there frequently is increased vascularity in the lym- phedematous limb, and inammatory cells accumulate. The affected limb has an increased sensitivity to fungal and bacter- ial infections. Obstructive lymphangitis further destroys the lymphatic system and results in progression of the lymphede- ma. In long-standing, neglected lymphedema, irreversible sclerosis of the subcutaneous tissue and skin develops. Lym- phangiosarcoma, which is a severe late complication of secondary lymphedema, fortunately is rare. Pathophysiology of chylous disorders Disorders in the circulation of chyle usually are caused by lym- phangiectasia or megalymphatics, with or without obstruc- tion of the thoracic duct (Fig. 18.8). 27,28 Because of valvular incompetence, chyle in these patients may reux to the pelvis or lower extremities, causing chylorrhea from small vesicles in the skin of the limb, scrotum, or labia (Fig. 18.9). Reux to the kidney may lead to chyluria, whereas transudation through or rupture of abdominal lymphatics results in chylous ascites. Rupture of the lymphatics into the lumen of the gut causes protein-losing enteropathy, and chylothorax develops if the PART I Vascular pathology and physiology 212 A B Figure 18.7 Lymphoscintigram in a 44-year-old woman with secondary lymphedema of the right lower extremity. (A) Note absence of right iliac nodes and the presence of right inguinal nodes and collaterals. (B) Note deterioration of lymphatic drainage 10 months later. There is no lling of the right inguinal nodes or collaterals. The patient had a recent episode of lymphangitis. Figure 18.8 Contrast lymphangiogram in an 18-year-old man with lymphangiectasia, protein-losing enteropathy, and chylous ascites demonstrates dilated and tortuous thoracic duct. thoracic duct or mediastinal, intercostal, or diaphragmatic lymphatics rupture. Secondary chyloperitoneum or chylothorax is caused most frequently by malignant tumors, primarily lymphoma, or by injury to the thoracic duct. The latter usually is iatrogenic, oc- curring during operations on the thoracoabdominal aorta 2931 or, rarely, after a high translumbar aortography. 32 Chyle is a sterile alkaline uid, odorless, and milky in ap- pearance. Its protein content is around 4g/dl and the fat con- tent ranges from 0.4 to 4g/dl. The fat stains with Sudan stain and this test conrms the diagnosis of chyle in the peritoneal or thoracic aspirate. The specic gravity of chylous uid is greater than 1012g/dl. Loss of chyle into the body cavities or through chylocuta- neous stulas has important physiologic consequences. If not treated, it leads to malnutrition, hypoproteinemia, hypo- cholesterolemia, hypocalcemia, immunodeciency, and se- vere metabolic disturbances. 27,28 Lymphopenia and anemia contribute to the poor immune function in these patients. Chylous effusion in a patient with malignancy usually CHAPTER 18 Physiologic changes in lymphatic dysfunction 213 A B Figure 18.9 (A) Chyle draining through small vesicles of the skin at the left groin of a 16-year- old girl with lymphangiectasia and severe reux of the chyle. (B) Intraoperative photograph of dilated, incompetent iliac lymphatics containing chyle. carries an ominous prognosis. The outcome of patients with primary chylous disorders and reux of the chyle depends on the effectiveness of medical treatment. To compensate for the physiologic changes caused by the loss of chyle, treatment is directed at decreasing production of the chyle with a medium- chain triglyceride diet, or by parenteral nutrition. In addition to adequate calorie and protein replacement, calcium, lost in chyle, also should be replaced. Reux can be controlled effec- tively with radical excision and ligation of the retroperitoneal lymphatics in most cases. In patients with chylous effusion, the site of lymphatic rupture should be oversewn if medical treatment, paracentesis, or thoracentesis are ineffective. In some patients with protein-losing enteropathy, the most dis- eased segment of the small bowel may have to be resected to decrease loss of chyle into the gastrointestinal tract. 27,28 Trans- plantation of small bowel for severe mesenteric lymphangiec- tasia remains a task of the future, and it requires, as do many other aspects of lymphatic disorders, further clinical research. References 1. Starling EH. 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