PAPER 3

Question 1

Question

1 (a)

Score

Explanation

Able to record all 5 time taken for the colour of iodine to

remain yellow, correctly
Test Tube
A
B
C
D
E

1 (b) (i)

Time taken for colour of iodine to

remain yellow
8.0 minutes
5.0 minutes
3.0 minutes
2.0 minutes
1.8 minutes

Able to state the correct observation based on following

criteria:
K1 Volume of saliva
K2 The time taken for the colour of iodine to remain
yellow
K3 Values
3

Able to state the observation based on all three criteria

correctly
Sample Answers
1. When 0.5 ml of saliva is used,the time taken for the
colour of iodine to remain yellow is 8 minutes.
2. Test tube E with 3.0 ml of saliva takes 1.8 minutes for
the colour of iodine to remain yellow
Able to state the observation based on any two criteria
correctly
When 0.5 ml of saliva is used, the time taken for colour of
iodine to remain yellow is longer

Remarks

5
3-4
1-2

- 3m
- 2m
- 1m

Question

Score

Explanation

Remarks

Able to state the observations based on any one criteria

correctly
Volume of saliva affect the time taken for colour of iodine to
remain yellow

1 (b) (ii)

No response or wrong response

Able to state two reasonable inferences for the

observations
Sample Answers
1. The less concentrated the enzyme used, the longer
the time taken to hydrolyse starch
2. The more concentrated the enzyme used, the
shorter the time taken to hydrolyse starch

Able to state two inferences but incomplete

1.The more concentrated enzyme used, the faster the
reaction of enzyme

Able to state any one inference or idea only

Sample Answers
1.The enzyme concentration affects the hydrolysis of starch

No response or wrong response

It has to
correspond
with the
observation

Question

Score

1 (c)

Explanation

Able to state all the variable correctly

Sample Answers
Suggested answer

How to operate variables

Manipulated
variable:

Method of changing the manipulated

variable:
Use differentvolume of saliva by using

Enzyme
concentration //
Volume of saliva
Responding
variable:

measuring cylinder
Determine what to observe in the
responding variable:
Measure and recordthe time taken for
iodine colour to remain yellow using a
stopwatch //

Time taken for

colour of iodine to
remain yellow//Rate
of enzyme reaction Calculate the rate of enzyme reaction
by using the formula : 1/ time taken
for iodine colour to remain yellow
Constant variable :

Volume of starch /
substrate // Time
interval for
observation

1 (d)

Method to fix the constant variable:

Fix/Maintain the volume of starch at 4

ml using the measuring cylinder

// Fix the time interval at 10 second
for observation by using a stopwatch

Able to state the hypothesis correctly based on the

following criteria:
K1- state the manipulated variable
K2- state the responding variable
K3- state the relationship between K1 and K2 (----er, higher)

Remarks

6
4-5
2-3

- 3m
- 2m
- 1m

Question

Score

Explanation

Remarks

Sample Answers
1) The higher the enzyme concentration , the higher the rate of
hydrolysis of starch //
2) The higher the volume of saliva, the shorter the time taken
for iodine colour to remain yellow
2

Able to state the hypothesis but with only 2 criteria.

Sample Answers
1) Enzymes concentration affects the rate of hydrolysis //
2) Volume of saliva affects the time taken for iodine colour to
remain yellow

Able to state the idea of the hypothesis

Sample Answers
1) Enzyme affects the rate of hydrolysis of starch
2) Saliva affects the time taken for iodine colour to remain
yellow

No response or wrong response

Any K1 or
K2 or K3

Question

Score

1 (e) (i)

Explanation

Able to construct a table and record the results the

experiment with following criteria:
K1- state all three aspects with units correctly
K2- transfer all data for enzyme concentration and
time taken for iodine colour to remain yellow
K3- states all the values for rate of reaction correctly.

3
Test
Tube

Enzyme
concentratio
n (%)

Time taken for

iodine colour to
remain yellow
(min)

16.70
A

8.0

0.20
5.0

50.00
C

0.33
3.0

66.70
D

Rate of
reaction
(min-1)
0.13

33.30

Remarks

0.50
2.0

Question

Score

Explanation

100.00
E

1 (e)(ii)

Remarks

0.56
1.8

Able to construct a table and record any two criteria

Able to construct a table and record any one criteria

No response or wrong response

Able to draw a graph of rate of reaction against the

amylase concentration which satisfies the following
criteria:
Axes (P) both axes are labelled and uniform scales,
manipulative variable on horizontal axis with units.
Points (T) all points correctly plotted
Shape (B) all points are connected smoothly

Question

1 (f)

Score

Explanation

Graph which satisfies any two criteria

Graph which satisfies any one criteria

No response or wrong response

Able to explain the relationship between the concentration

of enzymes and the rate of the hydrolysis of starch / rate of
reaction correctly
Sample Answers
K1 when the concentration of enzyme is high,
the rate of hydrolysis of starch is also high

Remarks

Has to
include all
the three K

K2 more active sites present (due to higher

concentration of enzyme)
K3 more starch is hydrolysed / broken down
2

Able to explain briefly the relationship between the

concentration of enzymes and the rate of the hydrolysis of
starch / rate of reaction correctly
Sample Answers
When the concentration of enzyme is high, more starch is
hydrolysed / broken down

Any 2K

K1 and K3

Question

Score

Explanation

Remarks

Able to explain the idea of the relationship between the

concentration of enzyme and the rate of hydrolysis of
starch / rate of reaction
Sample Answers
The higher the concentration of enzyme ,the higher the rate of Only K1 or
hydrolysis of starch.
K2 or K3

1 (g)

No response or wrong response

Able to predict correctly and explain the prediction based

on the following criteria:
K1- more concentrated enzyme is used
K2- more chance to hydrolyse substrate because has more
active sites
K3 the rate would be higher than 0.56 min-1 // Any suitable
value

Able to predict based on any two criteria

Able to predict based on any one criteria

Question

1 (h)

Score

Explanation

No response or wrong response

Able to state the definition of enzyme operationally,

complete and correct based on the following criteria:
K1 - A substance in saliva
K2 Responding variable
K3 - Manipulated variable
A substance in saliva that affect the time taken for iodine
colour to remain yellow and it depends on the concentration
of enzyme

Able to state the definition of enzyme based on any two

criteria

Able to state the definition of enzyme is based on any one

criteria or by theory

No response or wrong response

Remarks

Question

1 (i)

Score

Explanation

Able to list all the materials and apparatus correctly

Material

Apparatus

Water Bath
1% of starch solution

Test tubes
Iodine solution

Syringe,
Saliva solution

Dropper,

White tile with grooves

10

Remarks

Question

Questio
n

11

Score

Explanation

Able to record 3 materials and any 3 apparatus correctly

Able to record 2 materials and 2 apparatus correctly

No response or wrong response

Score

Explanation

Remarks

Remarks

Pernyataa
n Masalah

Boleh menyatakan pernyataan masalah dengan betul:

MV : Kepekatan larutan sukrosa
RV : Perubahan panjang//Perubahan berat keratan
kentang
H : Tanda tanya
Contoh jawapan:
1. Berapakah kepekatan sukrosa yang isotonik terhadap
sap sel kentang?
2. Apakah kesan larutan sukrosa yang berbeza ke atas
jisim/berat keratan kentang?
Boleh menyatakan pernyataan masalah tetapi kurang tepat:

Contoh jawapan:
1. Bagaimanakah larutan sukrosa mempengaruhi
perubahan jisim/panjang kentang.
Menyatakan idea sahaja

Question

Contoh jawapan:
1. Adakah larutan sukrosa memberi kesan terhadap
jisim/panjang kentang?

Tiada jawapan atau jawapan salah

Score

Explanation

Hipotesi
s

Boleh menyatakan hipotesis dengan betul :

MV= Kepekatan larutan sukrosa
RV = Perubahan Jisim//panjang keratan kentang
H = Perkaitan.

12

Contoh jawapan :
1. 8% (atau mana-mana angka) kepekatan sukrosa
adalah isotonik terhadap kepekatan sap sel kentang.

Remarks

Boleh menyatakan hipotesis tetapi kurang tepat.

2

Contoh jawapan:
1. Kepekatan larutan sukrosa yang berbeza memberi
kesan yang berbeza terhadap jisim/panjang kentang.
2. Semakin tinggi kepekatan sukrosa, semakin rendah
jisim/pendek keratan kentang.
Boleh menyatakan idea

Question

Contoh jawapan:
1. Kepekatan sukrosa memberi kesan terhadap
perubahan panjang/jisim kentang.

Tiada jawapan atau jawapan salah

Score

Explanation

Pembole
h ubah
3

Pembolehubah di manispulasi
= Kepekatan larutan sukrosa
Pembolehubah bergerak balas
= Perubahan jisim//Perubahan berat keratan kentang
Pembolehubah dimalarkan
= Jisim awal//Panjang awal keratan kentang// Tempoh masa

13

Remarks

Questio
n

Boleh menyatakan mana-mana dua pemboleh ubah.

Boleh menyatakan mana-mana satu pemboleh ubah.

Tiada jawapan atau jawapan salah

Score

Explanation

Senarai Alatan dan Bahan

3
Bahan:
Kentang, larutan sukrosa, Air suling

14

Alatan:
Penebuk gabus, piring petri, pisau, jam randik, kertas turas,
neraca elektronik/pembaris plastic.
3B + Alatan tidak lengkap

2B + Alatan tidak lengkap

Remarks

Questio
n

0B

Score

Kaedah
K1: Langkap menyediakan bahan dan alatan
menjalankan K2: Langkah menguruskan PU dimalarkan
eksperimen K3: Langkah menguruslkan PU dimanipulasikan
K4: Langkah menguruskan PU bergerakbalas
K5: Langkah mengikut urutan yang betul/sesuai
3

15

Explanation

1. Tebuk kentang menggunakan penebuk gabus.

2. Potong keratan kentang sepanjang 5 cm.
(Timbang berat awal kentang).
3. Masukkan 10 cm3 larutan sukrosa 5% ke
dalam piring petri.
4. Masukkan keratan kentang dan mulakan jam
randik.
5. Selepas 30 minit, ukur panjang akhir keratan
kentang. (Timbang berat akhir keratan
kentang).
6. Ulangi langkah 1 hingga 5 menggunakan
larutan sukrosa 0%, 10%, 20% dan 30 %.
7. Catatkan ukuran awal dan akhir dalam jadual.
8. Kira perbezaan bacaan awal dan akhir.
9. Bina graf garisan perbezaan panjang melawan
kepekatsan sukrosa.
10. Berdasarkan graf tentukan titik kepekatan
isotonik.
Mana-mana 3-4K

Remarks

Question

Mana-mana 2K

1K atau jawapan salah

Score

Explanation

Pemerhatian2
Kepekatan
larutan
sukrosa
(%)
0

10

16

Remarks

Bacaan 1 markah untuk lajur

awal MV
(cm/g) 1 markah untuk baris
RV

20

30

17