The Beckman/Coulter LH750 is an in vitro analytic instrument that provides complete blood cell

counts (CBC), white cell differential counts, and Reticulocyte Counts on whole blood.
DETERMINATION OF CELL BLOOD COUNT
Principle:
CBC Analysis The Coulter

method accurately counts and sizes cells by detecting and

measuring changes in electrical resistance when a particle (such as a cell) in a conductive liquid
passes through a small aperture. Each cell suspended in a conductive liquid (diluent) acts as an
insulator. As each cell goes through the aperture, it momentarily increases the resistance of the
electrical path between the submerged electrodes on either side of the aperture. This causes a
measurable electronic pulse. For counting, the vacuum used to pull the diluted suspension of
cells through the aperture must be at a regulated volume. The number of pulses correlates to the
number of particles. The height of the electrical pulse is proportional to the cell volume.
Differential Analysis- As the sample, prepared for differential analysis, streams through the flow
cell these three measurements occur simultaneously on each individual white cell to classify it:
-Low-frequency current measures volume. -High-frequency current senses cellular internal
content through measuring changes in conductivity. -Light from the laser bouncing off the
individual WBC cells characterizes cellular surface, shape, and reflectivity.
Materials required:Blood sample collected in a vacutainer, barcode scanner, wooden stick , Beckman/Coulter
LH750 and rack.
Reagents used:1. Coulter LH Series 700 Diluent

2. Coulter LH Series PAK Reagent Kit contains Erythrolyse II and StabiLyse

3. Coulter CBC Lyse S III diff Lytic Reagent
4. Coulter Clenz Cleaning Agent
5. Coulter LH Series Retic PAK Reagent Kit
6. Latron Primer
7. Latron Control
Procedure:1. Check for the presence of blood clot in blood collected in vacutainer. It is done by
swirling the blood with the help of wooden stick.
2. Load the reagents into Beckman coutler 700.
3.

If no blood clot is present in blood samples then scan the barcode of the sample by using
barcode scanner. Load samples into the rack with the barcode label facing up. The
operator places specimen tubes, which can be identified by bar-code labels, into cassettes.
Each cassette and the tube positions in the cassette are identified by bar-code labels.

4. You can load up to 12 standard or Hemogard cassettes with 144 samples into the loading
bay at one time.
5. Place the rack securely into the loading bay on the right side of the instrument. The
instrument will automatically start cycling. The system automatically transports, mixes,
aspirates and processes specimens.
6. Review patient results. The instrument has been programmed with Review by
Exception.

A. Samples that pass through these filters will be Autovalidated and sent to CLICS
Instrument Review Release.
B. Samples that did not pass through the review criteria will be in the Review List
folder.
7. To review patient results, select the Patient Result icon on the Command Center.
8. Click on Patient File Folder icon located at the top of the screen. The patient results are
placed in folders based on status and Run Type.
9. Select the Review List folder. The patient results that did not pass through the review
criteria will be in this folder. Results are listed with the most recent at the top.
10. Highlight the patient results to be reviewed and then select the Results and Graphics
icon on the right side of the screen. Patient results will be displayed. The CBC Data
including histograms, and the Diff Data including cytograms, are available by selecting
the appropriate tabs on the patient results screen.
11. To release results to CLICS, select the Validate icon on the top of the screen.
12. Close out the patient result screen to return to the Review List folder.

1. EVALUATION OF CELL BLOOD COUNT (CBC)

TABLE: RESULT OF CBC OF THE BLOOD SAMPLE

Parameters

Parameters name

Value

unit

obtained

Reference

range

(approximate)

WBC

White blood cell count

11.2

N x 103 cells/L

9 to 11 x 103 cells/L

NE%

Neutrophil percent

84.6

50 to 60%

LY%

Lymphocyte percent

5.8

25 to 35%

MO%

Monocyte percent

7.3

5 to 10%

BA%

Basophil percent

0.2

0.5 to 1.5%

EO%

Eosinophil percent

2.1

%
Nx 103 cells/L

2 to 5%

NE#

Neutrophil number

9.4

1.8 - 7.7 x 103 cells/L

Nx 103 cells/L

LY#

Lymphocyte number

24 - 44 x 103 cells/L

0.7
Nx 103 cells/L

MO#

Monocyte number

3 - 6 x 103 cells/L

0.8
Nx 103 cells/L

BA#

Basophil number

0 to 1 x 103 cells/L

0.0
Nx 103 cells/L

EO#

Eosinophil number

0.2

0-3

x 103 cells/L

Nx 103 cells/L
RBC

Red Blood Cell count

5.27

4.5 to 5.5 x
106cells/L

Hemoglobin
HGB

11.4

g/dL

14 to 16 g/dL

36.9

28 to 55%

70.1

fL

80 to 90 fL

21.7

pg

26-34 pg

30.9

g/dL

32-37 g/d

16.4

12 to 14%

896

N*103 cells/L

280 to 320 x 103

Hematocrit
HCT
MCV
MCH
MCHC
RDW

Mean corpuscular
volume
Mean corpuscular
hemoglobin
Mean corpuscular
hemoglobin
concentration
Red cell distribution
width
Platelet count

PLT

cells/L
Mean platelet volume
MPV

8.1

fL

8 to 10 fL

*Reference value may vary according to sex, age and some other factors. Reference values listed
in above table are approximate values.

DATA HISTOGRAM
Following are the data histogram of red blood cells, white blood cells and platelets:-

Picture:- normal histogram of RBC

The normal red cell distribution curve is Gaussian (bell-shaped) and the peak of the curve should
fall within the normal MCV range of 80.0 - 100.0 fL. This is shown in Figure 1. The red cell
distribution curve will get wider as the red cells vary more in size, as seen in Figure 2. Thus, a
narrow distribution curve indicates a homogenous population of red cells;