Professional Documents
Culture Documents
No. 3
1859 -
YY x YY
100% YY
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100% Yy
Yy yellow
YY x Yy
Y y
50% YY 50%Yy
yy green
yy x yy
100% yy ,
Yy x yy
Yy x Yy
Y y
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50%Yy 50%yy,
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1869 1882 -
1903 -
1920s
1928 -
R-Strain w/o
Rough edged
colonies
HEAT KILLED
VIRULENT
HEAT KILLED
NON-VIRULENT to mice
NON-VIRULENT to mice
+
Heat killed virulent R
strain
=
Live R nonvirulent strain
Live S virulent
strains obtained
1944 - The answer to the question on transformation was obtained from the results
of the experiments of Oswald Avery, Colin MacLeod and Maclyn McCarty.
a. Partially purified DNA from the virulent S strain extract was mixed with
live R cells and introduced in mice, resulting in killed mice.
b. When polysaccharide from S strain was added to live R strain and then
introduced to mice, no mice death was observed.
c. The next question : Was the DNA extract used pure and were there no
contaminating proteins or RNA?
d. When the extract was treated with RNAse that destroy RNA, or with a
proteolytic enzyme that destroy proteins, transformation into S virulent
still occurred in both cases.
e. When extract was treated with DNAse that depolymerize DNA, no
transformation was observed.
Therefore, these suggest that the transforming principle was DNA or the
nucleic acid.
1945 The term molecular biology was used by WILLIAM ASTBURY when he
was referring to the study of chemical and physical structure of
macromolecules
1952 - The Blender Experiment of Alfred Hershey and Martha Chase
The experiment made use of a kitchen blender and showed that the DNA
was introduced into the bacterial cell and DNA was later multiplied and
packaged into new viral particles.
32
P labeled DNA
bacteriophage
bacteria
6. 19 6.
lysis
Labeled
with 32P
blending
incubation
1950 1953 Rosalind Franklin obtained improved X ray diffraction data using
purified DNA samples. Data suggest a 3.4 nm periodicity and a helical
structure
1953
Linus Pauling and Robert Corey used data of Rosalind Franklin to
suggest a triple helix DNA structure with phosphates at the center
1953
James Watson and Francis Crick used the data of R. Franklin and
Chargaff and proposed the double helix structure of the DNA
1955
The first complete sequence of the peptide hormone insulin was
obtained.
1957
Matthew Messelson & Frank Stahl demonstrated how DNA is
replicated in cells.
1960
The existence of the messenger RNA was confirmed.
The first complete sequence of an enzyme, ribonuclease was
reported.
1962
Nobel Prize for the DNA double helix shared by James Watson,
Francis Crick, Rosalind Franklin, and Maurice Wilkins.
1966
The triplet code of all living organisms was completed.
1960 - 1970s
DNA sequencing was already being developed.
1967
DNA ligase, the enzyme that catalyzes the formation of a
phospodiester bond between two DNA chains, or joining of DNA
fragments was first discovered.
1968
Meselson and Yuan first discovered restriction enzymes from E.coli
but the cleavage sites are not clear
1970s
The discovery of restriction enzymes, the enzymes that cut DNA and
ligase, enzymes used to connect double stranded DNA and the
discovery of these enzymes paved the way for the advances in
genetic engineering. Now there are about 900 restriction enzymes
from about 230 different bacterial species.
1971
The Ca Cl2 or heat shock protocol for transformation was developed.
1972
Stanford University with Berg, Jackson, Symons, Mertz, and Davis
described the joining or ligation of DNA molecules from different
sources.
Use of gel electrophoresis, EcoRI restriction enzyme, and ethidium
bromide for staining gels with DNA were developed.
1973
Boyer, Helling, Cohen, and Chang published their work showing the
DNA fragment could be ligated or introduced into a plasmid vector.
Genes or DNA from frog was transferred to E. coli while bacteria could
be transformed with mouse DNA.
1977
Two DNA sequencing methods emerged, the chain termination and
chemical degradation
1980
Patent on methods of cloning and transformation was awarded to
Herbert Boye and and Boye later became the founder of the
biotechnology company Genentech.
Bioinformatics or computer applications in biological science was born.
1982
BIOTECH UPDATES
(provided in NBECT 2012 souvenir program)
1.
http://www.sciencedaily.com/releases/2012/08/120821114746.htm
Stem Cells Can Become Anything, but Not Without This Protein
How do stem cells preserve their ability to become any type of cell in the
body? And how do they "decide" to give up that magical state and start
specializing?
University of Michigan Medical School team led by Yali Dou, Ph.D., show the
crucial role of a protein called Mof in preserving the 'stem-ness' of stem cells, and
priming them to become specialized cells in mice. Without Mof, embryonic stem
cells lost their self-renewal capability and started to differentiate."
Mof appears to control the process that actually allows cells to determine
which genes it wants to read -- a crucial function for stem-ness. Mof marks the
areas that need to stay open and maintains the potential to become anything," Dou
explains. Its crucial role in many species is hinted at by the fact that the gene to
make Mof has the same sequence in fruit flies and mice.
2.
http://www.sciencedaily.com/releases/2012/08/120821094034.htm
Alzheimer Protein Seems to Slow Down Neurotransmitter Production
How abnormal protein deposits in the brains of Alzheimer's patients disrupt
the signalling between nerve cells has now been reported by researchers in Bochum
and Munich, led by Dr. Thorsten Mller from the Medizinisches Proteom-Center of
the Ruhr-Universitt, in the journal Molecular and Cellular Proteomics. They
varied the amount of APP protein and related proteins associated with Alzheimer's
disease in cell cultures, and then analysed how this manipulation affected other
proteins in the cell. The result: the amount of APP present was related to the
amount of an enzyme that is essential for the production of neurotransmitters and
therefore for communication amongst nerve cells.
3. http://news.yahoo.com/teen-doing-well-2-years-stem-cell-windpipe231123124.html
Teen Doing Well 2 Years After Stem Cell Windpipe Transplant
The windpipe was stripped of the donor's cells down to the inert structure of
collagen. Tissue from the lining of Finn-Lynch's windpipe was implanted in the
new windpipe to kick-start the growth of a lining in the new windpipe. Two years
after he became the first child to receive a stem cell-supported trachea (windpipe)
transplant, a 13-year-old boy is able to breathe normally, has grown about four
inches taller, does not require any anti-rejection drugs and has returned to school.
4. http://news.yahoo.com/european-regulators-back-first-gene-therapydrug-123941621--sector.html
European regulators back first gene therapy drug
Use of gene therapy suffered a major setback when an Arizona teenager died
in a gene therapy experiment in 1999 and two French boys with SCID
developed leukemia in 2002. This time, European regulators have
recommended approval of the Western world's first gene therapy drug, after
rejecting it on three previous occasions. The disorder to be treated is
estimated to affect no more than one or two people per million, can cause
acute pancreatitis and death.
5. http://www.biotechdaily.com/genomics_proteomics/articles/294742175/
mirnas_show_promise_for_treatment_of_ovarian_cancer.html
miRNAs Show Promise for Treatment of Ovarian Cancer
Investigators at the Georgia Institute of Technology (Atlanta, GA, USA)
worked with two well-defined mRNA species, MiR-7 and MiR-128. They
conducted a series of controlled experiments in which these two miRNAs-previously implicated in ovarian cancer onset/progression--were individually
transfected into a well-defined ovarian cancer (HEY) cell line, and the
consequence on global patterns of gene expression was monitored using
microarray technology.
such as catheters, reducing the need for harsh disinfectants and antibiotics, which
helps slow the development of resistance in the bacteria that colonize those surfaces.
Under a microscope, the structure of the polymer coating resembles a sponge.
Positive charges on the surface attract bacteria like a magnetic because their surface
has a negative charge. The pores in the polymer then pull the bacteria in, rupturing
the organism's cellular walls and killing them.
8. http://news.discovery.com/tech/gm-mosquitoes-released-in-malaysiato-reduce-dengue-spread.html
GM Mosquitoes Released in Malaysia to Reduce Dengue Spread
In late December, the Institute for Medical Research in
Malaysia released about 6,000 genetically modified (GM)
mosquitoes into the country's Eastern forests. These are
male mosquitoes that carried a lethal gene that kills
offspring early on; any survivors are not fit for
reproduction. This was one way of greatly reducing the number of offspring
and hence the number of dengue-carriers in the next generation. Some
groups, including Greenpeace, worry that reducing the population of just one
species will only make it easier for other species to proliferate in the wild -and Aedes aegypti is not the only dengue carrier.
9. http://news.discovery.com/tech/big-stink-technology-farms-120105.html
Bacteria Tech Halts Big Stink
Sulfur compounds produced industrially are what we
find most nauseating. These compounds can wrinkle human
noses with their cabbage-like stench at less than a part per
billion.A bacteria-filled filter is being perfected to prevent
bad industrial smells from spreading over a whole town.
Bacteria cover corrugated cellulose pads on the inside of an
odor biofilter used by pig farming operations in Denmark.
The filter contains corrugated cardboard filled with bacteria that are placed
inside machines. A large amount of air from the industrial site is then
pumped through the machines hourly, and a water rinse provides optimal
moist conditions. As the air flows through the machine, the bacteria process
the smelliest -- and most toxic -- compounds. With better efficiency and
improved design, the filters are getting close to costing less than a dollar per
pig to remove most odors it produces.
10. http://news.discovery.com/tech/poo-powered-bacteria-power-housebio-light-philips-111128.html
Poo-Powered Glowing Bacteria Light Up the House
Bio-light, a greener lighting system that's part of their
Microbial Home (MH) system. It isn't powered by electricity or
sunlight, but by glowing bioluminescent bacteria that thrive on
waste generated in the average home. The bioluminescent