Scribd Logo
Upload

Welcome to Scribd!

  • II.2 Metode Counting Chamber

    Uploaded by

    anggun dwi apriliani
    24 views1 page
    This document describes the results of counting yeast cells using a counting chamber method at different dilutions: 1. A dilution of 10,000x found an average of 50.73 cells per square and a total of 1.2683 x 1011 yeast cells per ml in the original sample. 2. A dilution of 100,000x found an average of 5 cells per square and a total of 1.25 x 1011 yeast cells per ml in the original sample. 3. A dilution of 1,000,000x was also tested but the results were not shown.

    Original Description:

    perhitungan turbidity

    Original Title

    turbidity 2

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This document describes the results of counting yeast cells using a counting chamber method at different dilutions: 1. A dilution of 10,000x found an average of 50.73 cells per square and a total of 1.2683 x 1011 yeast cells per ml in the original sample. 2. A dilution of 100,000x found an average of 5 cells per square and a total of 1.25 x 1011 yeast cells per ml in the original sample. 3. A dilution of 1,000,000x was also tested but the results were not shown.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    24 views1 page

    II.2 Metode Counting Chamber

    Uploaded by

    anggun dwi apriliani
    This document describes the results of counting yeast cells using a counting chamber method at different dilutions: 1. A dilution of 10,000x found an average of 50.73 cells per square and a total of 1.2683 x 1011 yeast cells per ml in the original sample. 2. A dilution of 100,000x found an average of 5 cells per square and a total of 1.25 x 1011 yeast cells per ml in the original sample. 3. A dilution of 1,000,000x was also tested but the results were not shown.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    II.

    2 Metode Counting Chamber


    Massa Ragi = 2 gram
    Tabel II.2.1 Hasil Pengamatan Metode Counting Chamber pengenceran 10.000 x

    A
    44

    KOTAK
    B
    C
    D
    51
    45 52

    E
    65

    46

    45

    48

    50

    42

    55

    40

    55

    RUN

    TOTAL

    JUMLAH
    SEL/KOTAK

    257

    51,4

    58

    247

    49,4

    65

    257

    51,4
    =152,2

    Jumlah sel ragi rata-rata

    = 152,2:3 = 50,73 sel/ kotak

    Jumlah sel ragi

    = 50,73 x 25 = 1268,3 sel/ mm2


    = 1268,3 x 10 = 12683 sel/mm3

    Jumlah sel ragi pada pengenceran 10.000x = 12683 x 103 = 1,2683 x 107 sel/ ml
    Jadi jumlah sel ragi pada larutan awal adalah = 1,2683 x 107 x 104
    = 1,2683 x 1011 sel/ ml sampel
    Tabel II.2.2 Hasil Pengamatan Metode Counting Chamber pengenceran 100.000 x

    A
    8

    KOTAK
    B
    C
    D
    4
    2
    5

    E
    7

    RUN

    TOTAL

    JUMLAH
    SEL/KOTAK

    26

    5,2

    24

    4,8

    25

    5
    =15

    Jumlah sel ragi rata-rata

    = 15:3 = 5 sel/ kotak

    Jumlah sel ragi

    = 5 x 25 = 125 sel/ mm2


    = 125 x 10 = 1250 sel/ mm3

    Jumlah sel ragi pada pengenceran 100.000x = 1250 x 103 =1,25 x 106 sel/ml
    Jadi jumlah sel ragi pada larutan awal adalah = 1,25 x 106 x 105
    = 1,25 x 1011 sel/ ml sampel
    Tabel II.2.3 Hasil Pengamatan Metode Counting Chamber pengenceran 1000.000 x

    You might also like