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TWEEN 80-AGAR: Solution A
TWEEN 80-AGAR: Solution A
TWEEN 80-AGAR
Solution A:
Peptone
NaCl
CaCl2 x 2 H2O
Agar
Destilled water
10.0
5.0
0.1
15.0
900.0
g
g
g
g
ml
Solution B:
Tween 80
Destilled water
10.0
100.0
g
ml
Autoclave solutions A and B separately and combine after cooling to approximately 50C.