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884.

TWEEN 80-AGAR

Solution A:
Peptone
NaCl
CaCl2 x 2 H2O
Agar
Destilled water

10.0
5.0
0.1
15.0
900.0

g
g
g
g
ml

Solution B:
Tween 80
Destilled water

10.0
100.0

g
ml

Autoclave solutions A and B separately and combine after cooling to approximately 50C.

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