Professional Documents
Culture Documents
1.1
Classification of Microorganisms
Microorganis
ms
bacteria
fungi
protozoa
Viruses
algae
Bacteria
1. Are very simple unicellular organisms which are most abundant
Structure
Glycogen
granule
Nucleic
material
Flagellum
Cell membrane
Slimy capsule
Cell wall
Cytoplasm
Characteristics
Function/characteristics
To store food
Cause gonorrhoea
Bacillus
Spirillu
m
Parasitism
Obtain nutrients from
another living organisms
(host)
Vibrio
Examples:
Tuberculosis bacteria
obtain food from patients
lung tissues
Cause vomiting and diarrhea
NOTE:
saprophytism are heterotrophs
parasitism and
(e) Respiration:
Aerobic respiration
Need oxygen to respire
Anaerobic respiration
Bacteria that do not need oxygen to
respire
(f) Reproduction:
Asexually (binary fission): at favourable environment
Asexually (Sporation): at unfavourable environment
Sexually (Conjugation): at unfavourable environment
binary fission
Conjugation
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Asexual reproduction
Sexual reproduction
At favourable environment
to another
Sporation
Unfavourable environment: extreme temperature, drought & lack of food:
Bacterial cell wall forms spores
Cell wall becomes thick and hibernate
When condition becomes favourable again, the bacterial cell wall splits open
and the bacteria grow and reproduce through binary fission
Fungi
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Characteristics:
(a) Location: dark and damp place such as decaying organic matter or in the
living organisms
(b) Are heterotrophs (obtain food through saprophytism and parasitism)
Saprophytism
Live on dead hosts Absorb nutrients
from decomposed tissues of the host with
the help of hyphae
Example:
Mucor
(c) Reproduction:
Parasitism
Parasites which live on another living
hosts and absorbs nutrients from the host
Examples:
Bracket fungi from tree bark
Phytophora species from potato plants
Asexual reproduction
Spore formation (cell division)
- At the end of the vertical hyphae
- Cell division occurs in the sporangium to produce
many spores
- When the sporangium is ripe, it bursts open and
disperse the spores and lands in a suitable place, it
germinates to produce a new mycelium
Sexual reproduction
Conjugation
Takes place when
surrounding condition is
not suitable
Fusion takes place
between hyphae to form a
spore which can withstand
unfavourable condition
Budding
- A bud that is formed on the parental cells and it grows
into a new cell.
- Occurs when there is a good supply of food
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SPM F5 SC Chapter 1
Protozoa
1. Biggest unicellular microorganisms
Characteristics:
(a) Has animal characteristics
Shapes
Location
Nutrition
Reproductio
n
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SPM F5 SC Chapter 1
Viruses
1. Smallest microorganisms
seen from electron microscope
2. Live inside the hosts (Parasitism)
3. Different from bacteria because they:
(a) Do not have nucleus, cytoplasm, cell membranes and cell organelles
(b) Do not carry out respiration, nutrition, response to stimuli, growth and
excretion and reproduction
(c) Can be crystallized
4. Structure:
(a) Consists of genetic material that made up of either DNA or RNA and
has protein coat that surrounds it
(b) Shapes: rod, spherical, tadpole-shaped and cubic
5. Causes disease in human, animals and plants
Eg: AIDS, influenza
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SPM F5 SC Chapter 1
Algae
1. Simple plant which do not have real root, stems and leaves
2. May be unicellular (Chlamydomonas) and multicellular (Spirogyra)
3. Location: bright, damp or moist (eg: soil and tree barks, soil, fresh water or
sea water)
4. Structure:
(a) A nucleus
(b) Cytoplasm
(c) Cellulose cell wall
(d) Chloroplast
(e) Flagella
5. Nutrition: autotrophs
6. Reproduction
Asexual reproduction: binary fission, spore formation and
fragmentation
Sexual reproduction: conjugation
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Bacterium
Fungus
Shape
0.2-10
Sphere, rod,
spiral, comma
10-100
Various
shapes
Unicellular
Unicellular/multicel
lular
Reproduction
method
Binary fission
Conjugation
Nutrition
Habitat
Protozoan
Virus
Alga
5-250
Various shape
Can change
shapes
Unicellular
0.02-0.4
Rod, shape,
cuboid, vibrio
spore formation
budding
Conjugation
Binary fission
Conjugation
Multiply inside of
a host
spore formation
budding
Conjugation
Binary fission
Fragmentation
Parasitism
Saprophytism
Autotrophism
Parasitism
Saprophytism
Parasitism
Saprophytism
Autotrophism
Parasitism
Autotrophism
Damp places
Damp places
Water/soil/in
living organisms
In living cells
Damp/wet places
Non-cellular
1-10 000
Oval, round,
filamentous ,
network
Unicellular
Multicellular
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SPM F5 SC Chapter 1
1.2
Temperature
Microorganism are in active at
low temperature
Nutrients
Nutrients
To provide energy
for growth
37 C is the optimum
temperature for most
microorganism growth and
reproduction
Temperatu
re
Humidity
Factors
affecting
micrrorgani
sm growth
pH Value
Types:
(a) Saprophytes
(b) Parasites
(c) Autotrophs
Light
Microorganisms ae killed at
high temperature but spores
are only killed at 120 C
pH Value
LIGHTS
pH 7 = most suitable pH
value for microorganisms
growth
Except Lactobacillus =
acidic condition
Microorganism needs
water for biochemical
Humidity processes
When
thebiochemical
environment is
Water
= for
dry, the microorganism
processes
will change into spores
Optimum
humidity =
25-he
and hibernate
until
40%
environment is
favourable
When environment is
dry: sporation occurs
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SPM F5 SC Chapter 1